Volume 270,
Number 20,
Issue of May 19, pp. 11903-11911, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
The
3`-Untranslated Region of Membrane Exon 2 from the 
2a
Immunoglobulin Gene Contributes to Efficient Transcription Termination
John A.
Flaspohler
,
David
Boczkowski
,
Brenda L.
Hall
,
Christine
Milcarek
Elements of the mouse Immunoglobulin 
2a gene, near the
membrane-specific poly(A) addition site, were inserted into a
heterologous location in either a synthetic mouse 2b gene or a
gpt/SV40 chimeric gene and then assayed for their ability to
terminate RNA polymerase II transcription in isolated nuclei of
transfected myeloma cells. The intact 2a membrane-specific
3`-untranslated region, with its potential stem loop forming sequences
and poly(A) site, is able to efficiently terminate transcription in the
absence of the downstream region in which transcription normally
terminates (term). Termination efficiency in the presence of the
termination fragment decreases either when sequences specifying a
potential stem/loop, upstream of the poly(A) region, are interrupted or
when the stronger membrane poly(A) site is substituted with a weaker,
secretory-specific poly(A) site. We therefore conclude that the 2a
membrane-specific untranslated region plays a major role in specifying
downstream termination. We further conclude that the immunoglobulin
2a, membrane-specific, 3`-untranslated region can function in the
context of the gpt gene, driven by an SV40 promoter, to
terminate transcription in a poly(A) site dependent fashion.
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