|
|
|
|||||||
|
|||||||||
Type 1 iodothyronine deiodinase (D1) is a microsomal
selenoenzyme which catalyzes deiodination of thyroxine to
3,5,3`-triiodothyronine. Immunoblotting showed that endogenous hepatic,
renal, and transiently expressed D1 remains in microsomes after pH 11.5
treatment. In vitro translation studies using pancreatic
microsomes identified a single transmembrane domain with a cytosolic
carboxyl-terminal catalytic portion. The transmembrane domain is
located between conserved basic amino acids at positions 11 and 12 and
a group of charged residues at positions 34-39. A transiently
expressed D1 protein in which residues 2-25 were deleted was
inactive and not integrated into membranes. Activity was not restored
by replacing these residues with transmembrane domains from a
cytochrome P450 or type 3 deiodinase enzyme despite their incorporation
into membranes. Elimination of the positive charges at positions 11 and
12 reduced the amount of transiently expressed protein by 70%, but the
enzyme formed was catalytically normal. Similar results were found
after conversion of the Lys-27 in the transmembrane domain to Met or
Glu. We conclude that the amino terminus of D1 contains uncleaved
signal and stop transfer sequence properties. In addition, positively
charged residues at positions 11, 12, and 27 are required for optimal
formation of the protein but not for catalysis.
Volume 270,
Number 20,
Issue of May 19, pp. 12310-12318, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. D. V. Sagar, B. Gereben, I. Callebaut, J.-P. Mornon, A. Zeold, C. Curcio-Morelli, J. W. Harney, C. Luongo, M. A. Mulcahey, P. R. Larsen, et al. The Thyroid Hormone-Inactivating Deiodinase Functions as a Homodimer Mol. Endocrinol., June 1, 2008; 22(6): 1382 - 1393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kohrle, F. Jakob, B. Contempre, and J. E. Dumont Selenium, the Thyroid, and the Endocrine System Endocr. Rev., December 1, 2005; 26(7): 944 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. H. M. Klaren, R. Haasdijk, J. R. Metz, L. M. C. Nitsch, V. M. Darras, S. Van der Geyten, and G. Flik Characterization of an Iodothyronine 5'-Deiodinase in Gilthead Seabream (Sparus auratus) that Is Inhibited by Dithiothreitol Endocrinology, December 1, 2005; 146(12): 5621 - 5630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. G J M Kuiper, W. Klootwijk, and T. J Visser Expression of recombinant membrane-bound type I iodothyronine deiodinase in yeast J. Mol. Endocrinol., June 1, 2005; 34(3): 865 - 878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. A. Gouveia, M. A. Christoffolete, C. R. Zaitune, J. M. Dora, J. W. Harney, A. L. Maia, and A. C. Bianco Type 2 Iodothyronine Selenodeiodinase Is Expressed throughout the Mouse Skeleton and in the MC3T3-E1 Mouse Osteoblastic Cell Line during Differentiation Endocrinology, January 1, 2005; 146(1): 195 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Shepherdley, W. Klootwijk, K. W. Makabe, T. J. Visser, and G. G. J. M. Kuiper An Ascidian Homolog of Vertebrate Iodothyronine Deiodinases Endocrinology, March 1, 2004; 145(3): 1255 - 1268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. G. J. M. Kuiper, F. Wassen, W. Klootwijk, H. van Toor, E. Kaptein, and T. J. Visser Molecular Basis for the Substrate Selectivity of Cat Type I Iodothyronine Deiodinase Endocrinology, December 1, 2003; 144(12): 5411 - 5421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Callebaut, C. Curcio-Morelli, J.-P. Mornon, B. Gereben, C. Buettner, S. Huang, B. Castro, T. L. Fonseca, J. W. Harney, P. R. Larsen, et al. The Iodothyronine Selenodeiodinases Are Thioredoxin-fold Family Proteins Containing a Glycoside Hydrolase Clan GH-A-like Structure J. Biol. Chem., September 19, 2003; 278(38): 36887 - 36896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. T. Nguyen, K. A. Mol, and J. J. DiStefano III Thyroid hormone production rates in rat liver and intestine in vivo: a novel graph theory and experimental solution Am J Physiol Endocrinol Metab, July 1, 2003; 285(1): E171 - E181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Curcio-Morelli, B. Gereben, A. M. Zavacki, B. W. Kim, S. Huang, J. W. Harney, P. R. Larsen, and A. C. Bianco In Vivo Dimerization of Types 1, 2, and 3 Iodothyronine Selenodeiodinases Endocrinology, March 1, 2003; 144(3): 937 - 946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Baqui, D. Botero, B. Gereben, C. Curcio, J. W. Harney, D. Salvatore, K. Sorimachi, P. R. Larsen, and A. C. Bianco Human Type 3 Iodothyronine Selenodeiodinase Is Located in the Plasma Membrane and Undergoes Rapid Internalization to Endosomes J. Biol. Chem., January 3, 2003; 278(2): 1206 - 1211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Bianco, D. Salvatore, B. Gereben, M. J. Berry, and P. R. Larsen Biochemistry, Cellular and Molecular Biology, and Physiological Roles of the Iodothyronine Selenodeiodinases Endocr. Rev., February 1, 2002; 23(1): 38 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Steinsapir, A. C. Bianco, C. Buettner, J. Harney, and P. R. Larsen Substrate-Induced Down-Regulation of Human Type 2 Deiodinase (hD2) Is Mediated through Proteasomal Degradation and Requires Interaction with the Enzyme's Active Center Endocrinology, March 1, 2000; 141(3): 1127 - 1135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Sanders, S. Van der Geyten, E. Kaptein, V. M. Darras, E. R. Kühn, J. L. Leonard, and T. J. Visser Cloning and Characterization of Type III Iodothyronine Deiodinase from the Fish Oreochromis niloticus Endocrinology, August 1, 1999; 140(8): 3666 - 3673. [Abstract] [Full Text]
|
|
|
|
|
|
B. Gereben, T. Bartha, H. M. Tu, J. W. Harney, P. Rudas, and P. R. Larsen Cloning and Expression of the Chicken Type 2 Iodothyronine 5'-Deiodinase J. Biol. Chem., May 14, 1999; 274(20): 13768 - 13776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D Prabakaran, R. Ahima, J. Harney, M. Berry, P. Larsen, and P Arvan Polarized targeting of epithelial cell proteins in thyrocytes and MDCK cells J. Cell Sci., January 4, 1999; 112(8): 1247 - 1256. [Abstract] [PDF]
|
|
|
|
|
|
J. P. Sanders, S. Van der Geyten, E. Kaptein, V. M. Darras, E. R. Kuhn, J. L. Leonard, and T. J. Visser Characterization of a Propylthiouracil-Insensitive Type I Iodothyronine Deiodinase Endocrinology, December 1, 1997; 138(12): 5153 - 5160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Toyoda, E. Kaptein, M. J. Berry, J. W. Harney, P. R. Larsen, and T. J. Visser Structure-Activity Relationships for Thyroid Hormone Deiodination by Mammalian Type I Iodothyronine Deiodinases Endocrinology, January 1, 1997; 138(1): 213 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Leonard, T. J. Visser, and D. M. Leonard Characterization of the Subunit Structure of the Catalytically Active Type I Iodothyronine Deiodinase J. Biol. Chem., January 19, 2001; 276(4): 2600 - 2607. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |