HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Babitzke, P. Articles by Yanofsky, C. Search for Related Content PubMed PubMed Citation Articles by Babitzke, P. Articles by Yanofsky, C. Social Bookmarking                      What's this?

Volume 270, Number 21, Issue of May 26, pp. 12452-12456, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Paul Babitzke , Charles Yanofsky

A filter binding assay was used to determine the structural features of L-tryptophan required for activation of TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis. We examined the ability of L-tryptophan and 26 of its analogs to activate TRAP. Our findings show that TRAP activation by L-tryptophan is highly cooperative. We also observed that TRAP activation is stereospecific; D-tryptophan did not activate. Our results further indicate that the -amino group and the carbonyl moiety of the -carboxyl group of the ligand are required for TRAP activation and that the heterocyclic amino nitrogen of L-tryptophan greatly enhances TRAP activation. We also found that changes at several positions of the indole ring of L-tryptophan resulted in reduced TRAP activation. In addition, indole and 5-methylindole were shown to be effective competitors of L-tryptophan activation of TRAP.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. P. McGraw, A. Mokdad, F. Major, P. C. Bevilacqua, and P. Babitzke Molecular basis of TRAP-5'SL RNA interaction in the Bacillus subtilis trp operon transcription attenuation mechanism RNA, January 1, 2009; 15(1): 55 - 66. [Abstract] [Full Text] [PDF]

				L. R. Cruz-Vera, M. Gong, and C. Yanofsky Physiological Effects of Anti-TRAP Protein Activity and tRNATrp Charging on trp Operon Expression in Bacillus subtilis J. Bacteriol., March 15, 2008; 190(6): 1937 - 1945. [Abstract] [Full Text] [PDF]

				M. Nie and H. Htun Different modes and potencies of translational repression by sequence-specific RNA-protein interaction at the 5'-UTR Nucleic Acids Res., November 14, 2006; 34(19): 5528 - 5540. [Abstract] [Full Text] [PDF]

				S. H. Najafi-Shoushtari, G. Mayer, and M. Famulok Sensing complex regulatory networks by conformationally controlled hairpin ribozymes Nucleic Acids Res., June 15, 2004; 32(10): 3212 - 3219. [Abstract] [Full Text] [PDF]

				R. Szigeti, M. Milescu, and P. Gollnick Regulation of the Tryptophan Biosynthetic Genes in Bacillus halodurans: Common Elements but Different Strategies than Those Used by Bacillus subtilis J. Bacteriol., February 1, 2004; 186(3): 818 - 828. [Abstract] [Full Text] [PDF]

				J. E. SCHAAK, P. BABITZKE, and P. C. BEVILACQUA Phylogenetic conservation of RNA secondary and tertiary structure in the trpEDCFBA operon leader transcript in Bacillus RNA, December 1, 2003; 9(12): 1502 - 1515. [Abstract] [Full Text] [PDF]

				A. Valbuzzi and C. Yanofsky Zinc Is Required for Assembly and Function of the Anti-trp RNA-binding Attenuation Protein, AT J. Biol. Chem., December 6, 2002; 277(50): 48574 - 48578. [Abstract] [Full Text] [PDF]

				P. T. X. Li and P. Gollnick Using Hetero-11-mers Composed of Wild Type and Mutant Subunits to Study Tryptophan Binding to TRAP and Its Role in Activating RNA Binding J. Biol. Chem., September 13, 2002; 277(38): 35567 - 35573. [Abstract] [Full Text] [PDF]

				P. T. X. Li, D. J. Scott, and P. Gollnick Creating Hetero-11-mers Composed of Wild-type and Mutant Subunits to Study RNA Binding to TRAP J. Biol. Chem., March 29, 2002; 277(14): 11838 - 11844. [Abstract] [Full Text] [PDF]

				A. Valbuzzi, P. Gollnick, P. Babitzke, and C. Yanofsky The Anti-trp RNA-binding Attenuation Protein (Anti-TRAP), AT, Recognizes the Tryptophan-activated RNA Binding Domain of the TRAP Regulatory Protein J. Biol. Chem., March 15, 2002; 277(12): 10608 - 10613. [Abstract] [Full Text] [PDF]

				P. Babitzke and P. Gollnick Posttranscription Initiation Control of Tryptophan Metabolism in Bacillus subtilis by the trp RNA-Binding Attenuation Protein (TRAP), anti-TRAP, and RNA Structure J. Bacteriol., October 15, 2001; 183(20): 5795 - 5802. [Full Text] [PDF]

				H. Yakhnin, J. E. Babiarz, A. V. Yakhnin, and P. Babitzke Expression of the Bacillus subtilis trpEDCFBA Operon Is Influenced by Translational Coupling and Rho Termination Factor J. Bacteriol., October 15, 2001; 183(20): 5918 - 5926. [Abstract] [Full Text] [PDF]

				H. Du, A. V. Yakhnin, S. Dharmaraj, and P. Babitzke trp RNA-Binding Attenuation Protein-5' Stem-Loop RNA Interaction Is Required for Proper Transcription Attenuation Control of the Bacillus subtilis trpEDCFBA Operon J. Bacteriol., April 1, 2000; 182(7): 1819 - 1827. [Abstract] [Full Text]

				A. V. Yakhnin, J. J. Trimble, C. R. Chiaro, and P. Babitzke Effects of Mutations in the L-Tryptophan Binding Pocket of the trp RNA-binding Attenuation Protein of Bacillus subtilis J. Biol. Chem., February 11, 2000; 275(6): 4519 - 4524. [Abstract] [Full Text] [PDF]

				S. Sudershana, H. Du, M. Mahalanabis, and P. Babitzke A 5' RNA Stem-Loop Participates in the Transcription Attenuation Mechanism That Controls Expression of the Bacillus subtilis trpEDCFBA Operon J. Bacteriol., September 15, 1999; 181(18): 5742 - 5749. [Abstract] [Full Text]

				C. Baumann, J. Otridge, and P. Gollnick Kinetic and Thermodynamic Analysis of the Interaction between TRAP (trp RNA-binding Attenuation Protein) of Bacillus subtilis and trp Leader RNA J. Biol. Chem., May 24, 1996; 271(21): 12269 - 12274. [Abstract] [Full Text] [PDF]