| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Mutations in the norpA gene of Drosophila
melanogaster severely affect the light-evoked photoreceptor
potential with strong mutations rendering the fly blind. The norpA gene has been proposed to encode phosphatidylinositol-specific
phospholipase C (PLC), which enzymes play a pivotal role in one of the
largest classes of signaling pathways known. A chimeric norpA minigene was constructed by placing the norpA cDNA behind
an R1-6 photoreceptor cell-specific rhodopsin promoter. This
minigene was transferred into norpA
Volume 270,
Number 22,
Issue of June 2, pp. 13271-13276, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
mutant by
P-element-mediated germline transformation to determine whether it
could rescue the phototransduction defect concomitant with restoring
PLC activity. Western blots of head homogenates stained with norpA
antiserum show that norpA protein is restored in heads of transformed
mutants. Moreover, transformants exhibit a large amount of measurable
PLC activity in heads, whereas heads of norpA
mutant
exhibit very little to none. Immunohistochemical staining of tissue
sections using norpA antiserum confirm that expression of norpA protein
in transformants localizes in the retina, more specifically in
rhabdomeres of R1-6 photoreceptor cells, but not R7 or R8
photoreceptor cells. Furthermore, electrophysiological analyses reveal
that transformants exhibit a restoration of light-evoked photoreceptor
responses in R1-6 photoreceptor cells, but not in R7 or R8
photoreceptor cells. This is the strongest evidence thus far supporting
the hypothesis that the norpA gene encodes phospholipase C
that is utilized in phototransduction.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. R. Spasic, P. Callaerts, and K. K. Norga Drosophila alicorn Is a Neuronal Maintenance Factor Protecting against Activity-Induced Retinal Degeneration J. Neurosci., June 18, 2008; 28(25): 6419 - 6429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Liu, R. Gnanasambandam, J. Benjamin, G. Kaur, P. B. Getman, A. J. Siegel, R. D. Shortridge, and S. Singh Mutations in Cytochrome c Oxidase Subunit VIa Cause Neurodegeneration and Motor Dysfunction in Drosophila Genetics, June 1, 2007; 176(2): 937 - 946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-F. Chen, J. Majercak, and I. Edery Clock-gated photic stimulation of timeless expression at cold temperatures and seasonal adaptation in Drosophila. J Biol Rhythms, August 1, 2006; 21(4): 256 - 271. [Abstract] [PDF]
|
|
|
|
 |
|
 M. del Pilar Gomez and E. Nasi A Direct Signaling Role for Phosphatidylinositol 4,5-Bisphosphate (PIP2) in the Visual Excitation Process of Microvillar Receptors J. Biol. Chem., April 29, 2005; 280(17): 16784 - 16789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H. Collins, E. Rosato, and C. P. Kyriacou Seasonal behavior in Drosophila melanogaster requires the photoreceptors, the circadian clock, and phospholipase C PNAS, February 17, 2004; 101(7): 1945 - 1950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Malpel, A. Klarsfeld, and F. Rouyer Larval optic nerve and adult extra-retinal photoreceptors sequentially associate with clock neurons during Drosophila brain development Development, March 5, 2003; 129(6): 1443 - 1453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bahner, P. Sander, R. Paulsen, and A. Huber The Visual G Protein of Fly Photoreceptors Interacts with the PDZ Domain Assembled INAD Signaling Complex via Direct Binding of Activated Galpha q to Phospholipase Cbeta J. Biol. Chem., January 28, 2000; 275(4): 2901 - 2904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schulz, A. Huber, K. Schwab, and R. Paulsen A Novel Ggamma Isolated from Drosophila Constitutes a Visual G Protein gamma Subunit of the Fly Compound Eye J. Biol. Chem., December 31, 1999; 274(53): 37605 - 37610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sakakibara, H. Inoue, and T. Yoshioka Evidence for the Involvement of Inositol Trisphosphate but Not Cyclic Nucleotides in Visual Transduction in Hermissenda Eye J. Biol. Chem., August 14, 1998; 273(33): 20795 - 20801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. d. P. Gomez and E. Nasi Membrane Current Induced by Protein Kinase C Activators in Rhabdomeric Photoreceptors: Implications for Visual Excitation J. Neurosci., July 15, 1998; 18(14): 5253 - 5263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Kim, D. S. Min, S. H. Ryu, and P.-G. Suh A Cytosolic, Galpha q- and beta gamma -insensitive Splice Variant of Phospholipase C-beta 4 J. Biol. Chem., February 6, 1998; 273(6): 3618 - 3624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Hardie INDO-1 Measurements of Absolute Resting and Light-Induced Ca2+ Concentration in Drosophila Photoreceptors J. Neurosci., May 1, 1996; 16(9): 2924 - 2933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Pearn, L. L. Randall, R. D. Shortridge, M. G. Burg, and W. L. Pak Molecular, Biochemical, and Electrophysiological Characterization of Drosophila norpA Mutants J. Biol. Chem., March 1, 1996; 271(9): 4937 - 4945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Broad, F.-J. Braun, J.-P. Lievremont, G. St. J. Bird, T. Kurosaki, and J. W. Putney Jr. Role of the Phospholipase C-Inositol 1,4,5-Trisphosphate Pathway in Calcium Release-activated Calcium Current and Capacitative Calcium Entry J. Biol. Chem., May 4, 2001; 276(19): 15945 - 15952. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
