| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The nine-subunit DNA polymerase (Pol) III* coupled to its
Volume 270,
Number 22,
Issue of June 2, pp. 13366-13377, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
III. INTERFACE BETWEEN TWO POLYMERASES AND THE CLAMP LOADER
sliding clamp is a rapid and highly processive replicating machine. The
multiple subunits are needed for the complicated task of duplicating
the Escherichia coli chromosome. In this report, Pol III* was
constituted from individual pure proteins, and its structure was
studied. Constitution of the Pol III* particle requires an ordered
addition of the subunits, and the final structure contains 14
polypeptides in the ratio
`
&khgr;.
The structure can be summarized as being composed of two core
polymerases () held together by a dimer of
and
one
complex clamp loader
(
`
&khgr;)
for loading onto DNA. At the center of the structure, the related
and
subunits form a heterotetramer upon which the two core
polymerases and clamp loader proteins assemble. The single copy nature
of the
,
`, &khgr;, and
subunits confers a structural
asymmetry with respect to the two polymerases, presumably for the
different functions of replicating the leading and lagging strands.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Chen, M. M. Coman, M. Sakato, M. O'Donnell, and M. M. Hingorani Conserved residues in the {delta} subunit help the E. coli clamp loader, {gamma} complex, target primer-template DNA for clamp assembly Nucleic Acids Res., June 1, 2008; 36(10): 3274 - 3286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Kadyrov, S. F. Holmes, M. E. Arana, O. A. Lukianova, M. O'Donnell, T. A. Kunkel, and P. Modrich Saccharomyces cerevisiae MutL{alpha} Is a Mismatch Repair Endonuclease J. Biol. Chem., December 21, 2007; 282(51): 37181 - 37190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jergic, K. Ozawa, N. K. Williams, X.-C. Su, D. D. Scott, S. M. Hamdan, J. A. Crowther, G. Otting, and N. E. Dixon The unstructured C-terminus of the {tau} subunit of Escherichia coli DNA polymerase III holoenzyme is the site of interaction with the {alpha} subunit Nucleic Acids Res., May 14, 2007; 35(9): 2813 - 2824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Anderson, C. R. Williams, M. O'Donnell, and L. B. Bloom A Function for the {psi} Subunit in Loading the Escherichia coli DNA Polymerase Sliding Clamp J. Biol. Chem., March 9, 2007; 282(10): 7035 - 7045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Neuwald Hypothesis: bacterial clamp loader ATPase activation through DNA-dependent repositioning of the catalytic base and of a trans-acting catalytic threonine Nucleic Acids Res., October 6, 2006; 34(18): 5280 - 5290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. L. de Saro, M. G. Marinus, P. Modrich, and M. O'Donnell The beta Sliding Clamp Binds to Multiple Sites within MutL and MutS J. Biol. Chem., May 19, 2006; 281(20): 14340 - 14349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Lo, E. van Der Schalie, T. Werner, Y. V. Brun, and N. Din A Temperature-Sensitive Mutation in the dnaE Gene of Caulobacter crescentus That Prevents Initiation of DNA Replication but Not Ongoing Elongation of DNA J. Bacteriol., February 15, 2004; 186(4): 1205 - 1212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Williams, A. K. Snyder, P. Kuzmic, M. O'Donnell, and L. B. Bloom Mechanism of Loading the Escherichia coli DNA Polymerase III Sliding Clamp: I. TWO DISTINCT ACTIVITIES FOR INDIVIDUAL ATP SITES IN THE {gamma} COMPLEX J. Biol. Chem., February 6, 2004; 279(6): 4376 - 4385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Neuwald Evolutionary clues to DNA polymerase III {beta} clamp structural mechanisms Nucleic Acids Res., August 1, 2003; 31(15): 4503 - 4516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Blinkova, M. J. Hermandson, and J. R. Walker Suppression of Temperature-Sensitive Chromosome Replication of an Escherichia coli dnaX(Ts) Mutant by Reduction of Initiation Efficiency J. Bacteriol., June 15, 2003; 185(12): 3583 - 3595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Johnson and M. O'Donnell Ordered ATP Hydrolysis in the gamma Complex Clamp Loader AAA+ Machine J. Biol. Chem., April 11, 2003; 278(16): 14406 - 14413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ason, R. Handayani, C. R. Williams, J. G. Bertram, M. M. Hingorani, M. O'Donnell, M. F. Goodman, and L. B. Bloom Mechanism of Loading the Escherichia coli DNA Polymerase III beta Sliding Clamp on DNA. BONA FIDE PRIMER/TEMPLATES PREFERENTIALLY TRIGGER THE gamma COMPLEX TO HYDROLYZE ATP AND LOAD THE CLAMP J. Biol. Chem., March 14, 2003; 278(12): 10033 - 10040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Kadyrov and J. W. Drake Characterization of DNA synthesis catalyzed by bacteriophage T4 replication complexes reconstituted on synthetic circular substrates Nucleic Acids Res., October 15, 2002; 30(20): 4387 - 4397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Bruck, A. Yuzhakov, O. Yurieva, D. Jeruzalmi, M. Skangalis, J. Kuriyan, and M. O'Donnell Analysis of a Multicomponent Thermostable DNA Polymerase III Replicase from an Extreme Thermophile J. Biol. Chem., May 3, 2002; 277(19): 17334 - 17348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. P. Leu and M. O'Donnell Interplay of Clamp Loader Subunits in Opening the beta Sliding Clamp of Escherichia coli DNA Polymerase III Holoenzyme J. Biol. Chem., December 7, 2001; 276(50): 47185 - 47194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yao, F. P. Leu, J. Anjelkovic, J. Turner, and M. O'Donnell DNA Structure Requirements for the Escherichia coli gamma Complex Clamp Loader and DNA Polymerase III Holoenzyme J. Biol. Chem., April 6, 2000; 275(15): 11440 - 11450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. P. Glover and C. S. McHenry The DnaX-binding Subunits delta ' and psi Are Bound to gamma and Not tau in the DNA Polymerase III Holoenzyme J. Biol. Chem., February 4, 2000; 275(5): 3017 - 3020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yao, J. Hurwitz, and M. O'Donnell Dynamics of beta and Proliferating Cell Nuclear Antigen Sliding Clamps in Traversing DNA Secondary Structure J. Biol. Chem., January 14, 2000; 275(2): 1421 - 1432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Janzen, M. Y. Torgov, and M. K. Reddy In Vitro Reconstitution of the Bacteriophage T4 Clamp Loader Complex (gp44/62) J. Biol. Chem., December 10, 1999; 274(50): 35938 - 35943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. K. O. Cann and Y. Ishino Archaeal DNA Replication: Identifying the Pieces to Solve a Puzzle Genetics, August 1, 1999; 152(4): 1249 - 1267. [Abstract] [Full Text]
|
|
|
|
|
|
M. M. Hingorani and M. O'Donnell ATP Binding to the Escherichia coli Clamp Loader Powers Opening of the Ring-shaped Clamp of DNA Polymerase III Holoenzyme J. Biol. Chem., September 18, 1998; 273(38): 24550 - 24563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. P. Glover and C. S. McHenry The chi psi Subunits of DNA Polymerase III Holoenzyme Bind to Single-stranded DNA-binding Protein (SSB) and Facilitate Replication of an SSB-coated Template J. Biol. Chem., September 4, 1998; 273(36): 23476 - 23484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Marians, H. Hiasa, D. R. Kim, and C. S. McHenry Role of the Core DNA Polymerase III Subunits at the Replication Fork. alpha IS THE ONLY SUBUNIT REQUIRED FOR PROCESSIVE REPLICATION J. Biol. Chem., January 23, 1998; 273(4): 2452 - 2457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Prescott and E. H. Blackburn Functionally interacting telomerase RNAs in the yeast telomerase complex Genes & Dev., November 1, 1997; 11(21): 2790 - 2800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Yurieva, M. Skangalis, J. Kuriyan, and M. O'Donnell Thermus thermophilis dnaX Homolog Encoding gamma - and tau -like Proteins of the Chromosomal Replicase J. Biol. Chem., October 24, 1997; 272(43): 27131 - 27139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Bloom, J. Turner, Z. Kelman, J. M. Beechem, M. O'Donnell, and M. F. Goodman Dynamics of Loading the beta Sliding Clamp of DNA Polymerase III onto DNA J. Biol. Chem., November 29, 1996; 271(48): 30699 - 30708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kim, H. G. Dallmann, C. S. McHenry, and K. J. Marians tau Couples the Leading- and Lagging-strand Polymerases at the Escherichia coli DNA Replication Fork J. Biol. Chem., August 30, 1996; 271(35): 21406 - 21412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Kim and C. S. McHenry Identification of the beta -binding Domain of the alpha Subunit of Escherichia coli Polymerase III Holoenzyme J. Biol. Chem., August 23, 1996; 271(34): 20699 - 20704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Pritchard, H. G. Dallmann, and C. S. McHenry In Vivo Assembly of the [IMAGE]-Complex of the DNA Polymerase III Holoenzyme Expressed from a Five-Gene Artificial Operon J. Biol. Chem., April 26, 1996; 271(17): 10291 - 10298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. G. Dallmann, R. L. Thimmig, and C. S. McHenry DnaX Complex of Escherichia coli DNA Polymerase III Holoenzyme J. Biol. Chem., December 8, 1995; 270(49): 29555 - 29562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. G. Dallmann and C. S. McHenry DnaX Complex of Escherichia coli DNA Polymerase III Holoenzyme J. Biol. Chem., December 8, 1995; 270(49): 29563 - 29569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Onrust, J. Finkelstein, V. Naktinis, J. Turner, L. Fang, and M. O'Donnell Assembly of a Chromosomal Replication Machine: Two DNA Polymerases, a Clamp Loader, and Sliding Clamps in One Holoenzyme Particle J. Biol. Chem., June 2, 1995; 270(22): 13348 - 13357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xiao, V. Naktinis, and M. O'Donnell Assembly of a Chromosomal Replication Machine: Two DNA Polymerases, a Clamp Loader, and Sliding Clamps in One Holoenzyme Particle J. Biol. Chem., June 2, 1995; 270(22): 13378 - 13383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. T. Stukenberg and M. O'Donnell Assembly of a Chromosomal Replication Machine: Two DNA Polymerases, a Clamp Loader, and Sliding Clamps in One Holoenzyme Particle J. Biol. Chem., June 2, 1995; 270(22): 13384 - 13391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Bruck and M. O'Donnell The DNA Replication Machine of a Gram-positive Organism J. Biol. Chem., September 8, 2000; 275(37): 28971 - 28983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. P. Leu, M. M. Hingorani, J. Turner, and M. O'Donnell The delta Subunit of DNA Polymerase III Holoenzyme Serves as a Sliding Clamp Unloader in Escherichia coli J. Biol. Chem., October 27, 2000; 275(44): 34609 - 34618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li and K. J. Marians Two Distinct Triggers for Cycling of the Lagging Strand Polymerase at the Replication Fork J. Biol. Chem., October 27, 2000; 275(44): 34757 - 34765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gao and C. S. McHenry tau Binds and Organizes Escherichia coli Replication Proteins through Distinct Domains. DOMAIN III, SHARED BY gamma AND tau , BINDS delta delta ' AND chi psi J. Biol. Chem., February 2, 2001; 276(6): 4447 - 4453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Stewart, M. M. Hingorani, Z. Kelman, and M. O'Donnell Mechanism of beta Clamp Opening by the delta Subunit of Escherichia coli DNA Polymerase III Holoenzyme J. Biol. Chem., May 25, 2001; 276(22): 19182 - 19189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Kadyrov and J. W. Drake Conditional Coupling of Leading-strand and Lagging-strand DNA Synthesis at Bacteriophage T4 Replication Forks J. Biol. Chem., July 27, 2001; 276(31): 29559 - 29566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Pritchard and C. S. McHenry Assembly of DNA Polymerase III Holoenzyme. CO-ASSEMBLY OF gamma AND tau IS INHIBITED BY DnaX COMPLEX ACCESSORY PROTEINS BUT STIMULATED BY DNA POLYMERASE III CORE J. Biol. Chem., September 7, 2001; 276(37): 35217 - 35222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. P. Glover, A. E. Pritchard, and C. S. McHenry tau Binds and Organizes Escherichia coli Replication Proteins through Distinct Domains. DOMAIN III, SHARED BY gamma AND tau , OLIGOMERIZES DnaX J. Biol. Chem., September 14, 2001; 276(38): 35842 - 35846. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-S. Song, H. G. Dallmann, and C. S. McHenry Carboxyl-terminal Domain III of the delta ' Subunit of the DNA Polymerase III Holoenzyme Binds delta J. Biol. Chem., October 26, 2001; 276(44): 40668 - 40679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. G. Dallmann, S. Kim, A. E. Pritchard, K. J. Marians, and C. S. McHenry Characterization of the Unique C Terminus of the Escherichia coli tau DnaX Protein. MONOMERIC C-tau BINDS alpha AND DnaB AND CAN PARTIALLY REPLACE tau IN RECONSTITUTED REPLICATION FORKS J. Biol. Chem., May 12, 2000; 275(20): 15512 - 15519. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
