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Volume 270, Number 23, Issue of June 9, pp. 13593-13595, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.

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DNA Enzymology above 100 C
TOPOISOMERASE V UNLINKS CIRCULAR DNA AT 80-122 °C

Sergei A. Kozyavkin , Alexander V. Pushkin , Frederick A. Eiserling , Karl O. Stetter , James A. Lake , Alexei I. Slesarev

The widespread application of polymerase chain reaction and related techniques in biology and medicine has led to a heightened interest in thermophilic enzymes of DNA metabolism. Some of these enzymes are stable for hours at 100 °C, but no enzymatic activity on duplex DNA at temperatures above 100 °C has so far been demonstrated. Recently, we isolated topoisomerase V from the hyperthermophile Methanopyrus kandleri, which grows up to 110 °C. This novel enzyme is similar to eukaryotic topoisomerase I and acts on duplex DNA regions. We now show that topoisomerase V catalyzes the unlinking of double-stranded circular DNA at temperatures up to 122 °C. In this in vitro system, maximal DNA unlinking occurs at 108 °C and corresponds to complementary strands being linked at most once. These results further imply that in the presence of sufficient positive supercoiling DNA can exist as a double helix even at 122 °C.

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