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Previous studies suggested that transcription enhancer factor-1
(TEF-1) was involved in mediating the human chorionic somatomammotropin
(hCS) gene enhancer (CSEn) function (Jiang, S.-W., and
Eberhardt, N. L.(1994) J. Biol. Chem. 269, 10384-10392).
We now show that an unrelated protein (CSEF-1) found in BeWo and COS-1
cells binds to the GT-IIC enhanson in CSEn and is correlated with CSEn
activity in these cells. TEF-1 and CSEF-1 were distinguished by
differential migration as GT-IIC complexes, thermal stability,
molecular mass, and cross-reactivity with chicken TEF-1 antibodies.
TEF-1 and CSEF-1 bound to the GT-IIC and Sph-I/Sph-II enhansons with
identical binding properties, and in vitro generated TEF-1
competed with CSEF-1 binding to the GT-IIC motif, suggesting that their
actions might be mutually exclusive. Up- and down-regulation of TEF-1
levels by expression systems and antisense oligonucleotides
demonstrated that TEF-1 inhibited the hCS promoter in a manner
independent of the enhancer or a known TEF-1 DNA binding site. The data
suggest that TEF-1 may provide a counter-regulatory stimulus to the
actions of CSEF-1, which may be involved in mediating enhancer
stimulatory activity.
Volume 270,
Number 23,
Issue of June 9, pp. 13906-13915, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
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