|
|
|
|||||||
|
|||||||||
Cell migration is a fundamental process of wound repair in
biological systems. In an attempt to identify plasma membrane
glycoconjugates which mediate cell migration, migrating and
nonmigrating rabbit corneal epithelia were analyzed for reactivity with
monoclonal antibodies (mAbs) specific for unsubstituted
N-acetyllactosamine (mAb 1B2), Le
Volume 270,
Number 23,
Issue of June 9, pp. 14015-14023, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
(mAbs 7A and
MMA), and sialyl Le (mAb CSLEX1) carbohydrate chains of
neolactoglycoconjugates. Immunohistochemical analyses indicated that
regardless of whether the epithelia analyzed were from corneas of
animals in vivo, corneas in organ culture, or cells in tissue
culture, migrating cells stained intensely with mAb 1B2, whereas
nonmigrating cells either did not stain or stained only weakly. mAbs
MMA and 7A stained migrating epithelium as well as basal and middle
cell layers of normal, nonmigrating epithelium. mAb CSLEX1 did not
stain wounded corneas but stained the superficial cell layer of normal
corneal epithelium. Biochemical analyses by TLC immunostaining revealed
the presence of three mAb 1B2-reactive glycosphingolipids (GSL),
neolactotetraosyl-(nLc
, paragloboside), neolactohexaosyl-
(nLc
), and neolacto-octaosylceramide (nLc
) in
migrating epithelia. In contrast, nonmigrating epithelia contained only
trace amounts of these glycolipids. Exogenous addition of
nLc, but not various other GSLs including a
Le-GSL (SSEA-1), stimulated re-epithelialization of wounds
in an experimental model of corneal epithelial wound healing. Moreover,
re-epithelialization of wounds was significantly inhibited by mAb 1B2
but not by mAb MMA. The data suggest that neolacto-GSLs of corneal
epithelium may be among the molecules which mediate healing of corneal
epithelial wounds by influencing cell migration.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. H. Levin and A. S. Verkman Aquaporin-3-Dependent Cell Migration and Proliferation during Corneal Re-epithelialization. Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4365 - 4372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Cao, N. Said, H. K. Wu, I. Kuwabara, F.-T. Liu, and N. Panjwani Galectin-7 as a Potential Mediator of Corneal Epithelial Cell Migration Arch Ophthalmol, January 1, 2003; 121(1): 82 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Cao, N. Said, S. Amin, H. K. Wu, A. Bruce, M. Garate, D. K. Hsu, I. Kuwabara, F.-T. Liu, and N. Panjwani Galectins-3 and -7, but not Galectin-1, Play a Role in Re-epithelialization of Wounds J. Biol. Chem., October 25, 2002; 277(44): 42299 - 42305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Cao, H. K. Wu, A. Bruce, K. Wollenberg, and N. Panjwani Detection of Differentially Expressed Genes in Healing Mouse Corneas, Using cDNA Microarrays Invest. Ophthalmol. Vis. Sci., September 1, 2002; 43(9): 2897 - 2904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Dorscheid, K. R. Wojcik, K. Yule, and S. R. White Role of cell surface glycosylation in mediating repair of human airway epithelial cell monolayers Am J Physiol Lung Cell Mol Physiol, October 1, 2001; 281(4): L982 - L992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Cao, D. M. Jefferson, and N. Panjwani Role of Carbohydrate-mediated Adherence in Cytopathogenic Mechanisms of Acanthamoeba J. Biol. Chem., June 19, 1998; 273(25): 15838 - 15845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Cao, Z. Zhao, R. Mohan, J. Alroy, P. Stanley, and N. Panjwani Role of the Lewisx Glycan Determinant in Corneal Epithelial Cell Adhesion and Differentiation J. Biol. Chem., June 8, 2001; 276(24): 21714 - 21723. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |