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cDNAs for two distinct Type III cGMP-inhibited (cGI) cyclic
nucleotide phosphodiesterases (PDE), designated cGIP1 and cGIP2, were
previously cloned from rat adipose and human cardiac cDNA libraries,
respectively. In this study, another cDNA (
Volume 270,
Number 24,
Issue of June 16, pp. 14305-14312, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
4.0 kilobase (kb))
encoding a cGI-PDE of 74 kDa (658 amino acids) was isolated from a
human placental cDNA library. The nucleotide sequence of its open
reading frame was virtually identical to a corresponding region in the
3` portion of the cardiac cGIP2 cDNA (
7.6 kb) which encoded a
125-kDa cGI-PDE (1141 amino acid). Northern blots and RNase
protection assays revealed a prominent 4.4-kb transcript and a 7.6-kb
transcript in human placenta. The transcription start site of the
4.4-kb transcript was assigned to cardiac cDNA nucleotide 1292, the
putative beginning of exon 3 of the human cGIP2 gene, with a potential
translation initiation site 183 bases downstream, as determined by
RNase protection assay. The 5`-flanking region of the 4.4-kb transcript
exhibited promoter activity in HeLa cells which expressed the 4.4-kb
transcript, and contained a TATAA sequence 35 base pairs upstream from
the tentative transcription start site. Recombinant cGI-PDEs, expressed
in Sf9 cells from the 7.6- and 4.0-kb cDNA, exhibited differences in
their subcellular localization and K
for
cGMP. Thus, in human tissues, alternative transcription may contribute
to generating at least two cGIP2 isoforms, cytosolic and
membrane-associated cGI-PDEs with different K
values for cGMP.
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