| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
cDNAs for two distinct Type III cGMP-inhibited (cGI) cyclic
nucleotide phosphodiesterases (PDE), designated cGIP1 and cGIP2, were
previously cloned from rat adipose and human cardiac cDNA libraries,
respectively. In this study, another cDNA (
Volume 270,
Number 24,
Issue of June 16, pp. 14305-14312, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
4.0 kilobase (kb))
encoding a cGI-PDE of 74 kDa (658 amino acids) was isolated from a
human placental cDNA library. The nucleotide sequence of its open
reading frame was virtually identical to a corresponding region in the
3` portion of the cardiac cGIP2 cDNA (
7.6 kb) which encoded a
125-kDa cGI-PDE (1141 amino acid). Northern blots and RNase
protection assays revealed a prominent 4.4-kb transcript and a 7.6-kb
transcript in human placenta. The transcription start site of the
4.4-kb transcript was assigned to cardiac cDNA nucleotide 1292, the
putative beginning of exon 3 of the human cGIP2 gene, with a potential
translation initiation site 183 bases downstream, as determined by
RNase protection assay. The 5`-flanking region of the 4.4-kb transcript
exhibited promoter activity in HeLa cells which expressed the 4.4-kb
transcript, and contained a TATAA sequence 35 base pairs upstream from
the tentative transcription start site. Recombinant cGI-PDEs, expressed
in Sf9 cells from the 7.6- and 4.0-kb cDNA, exhibited differences in
their subcellular localization and K
for
cGMP. Thus, in human tissues, alternative transcription may contribute
to generating at least two cGIP2 isoforms, cytosolic and
membrane-associated cGI-PDEs with different K
values for cGMP.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Omori and J. Kotera Overview of PDEs and Their Regulation Circ. Res., February 16, 2007; 100(3): 309 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wechsler, Y.-H. Choi, J. Krall, F. Ahmad, V. C. Manganiello, and M. A. Movsesian Isoforms of Cyclic Nucleotide Phosphodiesterase PDE3A in Cardiac Myocytes J. Biol. Chem., October 4, 2002; 277(41): 38072 - 38078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J. Richard, A. Tsafriri, and M. Conti Role of Phosphodiesterase Type 3A in Rat Oocyte Maturation Biol Reprod, November 1, 2001; 65(5): 1444 - 1451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shitsukawa, C.B. Andersen, F.J. Richard, A.K. Horner, A. Wiersma, M. van Duin, and M. Conti Cloning and Characterization of the Cyclic Guanosine Monophosphate-Inhibited Phosphodiesterase PDE3A Expressed in Mouse Oocyte Biol Reprod, July 1, 2001; 65(1): 188 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Smith, R. Huang, D. Sun, S. Ricketts, C. Hoegler, J.-Z. Ding, R. A. Moggio, and T. H. Hintze Development of Decompensated Dilated Cardiomyopathy Is Associated With Decreased Gene Expression and Activity of the Milrinone-Sensitive cAMP Phosphodiesterase PDE3A Circulation, November 4, 1997; 96(9): 3116 - 3123. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Shakur, K. Takeda, Y. Kenan, Z.-X. Yu, G. Rena, D. Brandt, M. D. Houslay, E. Degerman, V. J. Ferrans, and V. C. Manganiello Membrane Localization of Cyclic Nucleotide Phosphodiesterase 3 (PDE3). TWO N-TERMINAL DOMAINS ARE REQUIRED FOR THE EFFICIENT TARGETING TO, AND ASSOCIATION OF, PDE3 WITH ENDOPLASMIC RETICULUM J. Biol. Chem., December 1, 2000; 275(49): 38749 - 38761. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
