HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Milton, N. G. N. Articles by Latchman, D. S. Search for Related Content PubMed PubMed Citation Articles by Milton, N. G. N. Articles by Latchman, D. S. Social Bookmarking                      What's this?

Volume 270, Number 25, Issue of June 23, pp. 15143-15147, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.

---

The Neuronal Nicotinic Acetylcholine Receptor 2 Subunit Gene Promoter Is Activated by the Brn-3b POU Family Transcription Factor and Not by Brn-3a or Brn-3c

Nathaniel G. N. Milton , Alain Bessis , Jean-Pierre Changeux , David S. Latchman

The regulatory region of the neuronal nicotinic acetylcholine receptor 2 subunit gene, which contains six copies of the octamer-related sequence CCCCATGCAAT, is activated by the Brn-3b POU family transcription factor but not by the closely related factors Brn-3a and Brn-3c. This effect is in contrast to the previously documented inhibitory effect of Brn-3b on octamer-containing promoters that are activated by Brn-3a and Brn-3c. Activation of the 2 gene by Brn-3b requires that both the POU domain and other N-terminal sequences are derived from Brn-3b and is dependent on the intactness of the 2 gene regulatory region, being lost in truncated derivatives containing one, two, or four copies of the octamer-related sequence. Surprisingly, however, these truncated derivatives are activated by Brn-3c. These effects are discussed in terms of both the influence of the target sequence and its context in the promoter on activation by the various forms of Brn-3 as well as of the processes that restrict expression of the 2 subunit gene to a few cells in the nervous system.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J. Y. Sze and G. Ruvkun Activity of the Caenorhabditis elegans UNC-86 POU transcription factor modulates olfactory sensitivity PNAS, August 5, 2003; 100(16): 9560 - 9565. [Abstract] [Full Text] [PDF]

				C. Carrasco-Serrano, A. Campos-Caro, S. Viniegra, J. J. Ballesta, and M. Criado GC- and E-box Motifs as Regulatory Elements in the Proximal Promoter Region of the Neuronal Nicotinic Receptor alpha 7 Subunit Gene J. Biol. Chem., August 7, 1998; 273(32): 20021 - 20028. [Abstract] [Full Text] [PDF]

				Q. Du, I. N. Melnikova, and P. D. Gardner Differential Effects of Heterogeneous Nuclear Ribonucleoprotein K on Sp1- and Sp3-mediated Transcriptional Activation of a Neuronal Nicotinic Acetylcholine Receptor Promoter J. Biol. Chem., July 31, 1998; 273(31): 19877 - 19883. [Abstract] [Full Text] [PDF]

				C. B. Bigger, I. N. Melnikova, and P. D. Gardner Sp1 and Sp3 Regulate Expression of the Neuronal Nicotinic Acetylcholine Receptor beta 4 Subunit Gene J. Biol. Chem., October 10, 1997; 272(41): 25976 - 25982. [Abstract] [Full Text] [PDF]

				M. D. Smith, P. J. Morris, S. J. Dawson, M. L. Schwartz, W. W. Schlaepfer, and D. S. Latchman Coordinate Induction of the Three Neurofilament Genes by the Brn-3a Transcription Factor J. Biol. Chem., August 22, 1997; 272(34): 21325 - 21333. [Abstract] [Full Text] [PDF]

				Q. Du, A. E. Tomkinson, and P. D. Gardner Transcriptional Regulation of Neuronal Nicotinic Acetylcholine Receptor Genes. A POSSIBLE ROLE FOR THE DNA-BINDING PROTEIN Puralpha J. Biol. Chem., June 6, 1997; 272(23): 14990 - 14995. [Abstract] [Full Text] [PDF]

				D. Fornasari, E. Battaglioli, A. Flora, S. Terzano, and F. Clementi Structural and Functional Characterization of the Human alpha 3 Nicotinic Subunit Gene Promoter Mol. Pharmacol., February 1, 1997; 51(2): 250 - 261. [Abstract] [Full Text] [PDF]

				M. D. Smith, S. J. Dawson, and D. S. Latchman Inhibition of Neuronal Process Outgrowth and Neuronal Specific Gene Activation by the Brn-3b Transcription Factor J. Biol. Chem., January 10, 1997; 272(2): 1382 - 1388. [Abstract] [Full Text] [PDF]

				C. B. Bigger, E. A. Casanova, and P. D. Gardner Transcriptional Regulation of Neuronal Nicotinic Acetylcholine Receptor Genes. FUNCTIONAL INTERACTIONS BETWEEN Sp1 AND THE RAT beta 4 SUBUNIT GENE PROMOTER J. Biol. Chem., December 20, 1996; 271(51): 32842 - 32848. [Abstract] [Full Text] [PDF]