HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Guo, W.-W. Articles by Perlman, P. S. Search for Related Content PubMed PubMed Citation Articles by Guo, W.-W. Articles by Perlman, P. S. Social Bookmarking                      What's this?

Volume 270, Number 26, Issue of June 30, pp. 15563-15570, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.

---

The Mobile Group I Intron 3 of the Yeast Mitochondrial COXI Gene Encodes a 35-kDa Processed Protein That Is an Endonuclease but Not a Maturase

Wei-Wen Guo , John V. Moran , Peter W. Hoffman , R. Michael Henke , Ronald A. Butow , Philip S. Perlman

Three mitochondrial mutants were characterized that block the splicing of aI3, a mobile group I intron of the COXI gene of yeast mtDNA. Mutant C1085 alters helical structures known to be important for splicing of group I introns. M44 and C1072 are point mutants in exon 3 that block correct splicing but allow some splicing at cryptic 5`-splice sites. M44 alters the P1 helix needed for 5`-splice site definition, while the mutation in C1072 is a new kind of mutation because it is located upstream of the exon sequence involved in the P1 helix. All three mutants accumulate novel proteins of 35 and 44 kDa (p35 and p44, respectively) detected both by labeling of mitochondrial translation products and by Western blotting. Partial protease digestions indicate that p44 and p35 are closely related, probably as precursor and processed protein. The level of the intron-encoded endonuclease activity, I-SceIII, is elevated 10-fold in the mutants. Partial purification of I-SceIII from the mutants showed that most, if not all, of the activity is associated with p35. Finally, because aI3 splices accurately in a petite mutant, we conclude that aI3 splicing does not depend on a mtDNA-encoded maturase.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. H. Barros, A. M. Myers, S. Van Driesche, and A. Tzagoloff COX24 Codes for a Mitochondrial Protein Required for Processing of the COX1 Transcript J. Biol. Chem., February 10, 2006; 281(6): 3743 - 3751. [Abstract] [Full Text] [PDF]

				J. Lin and V. M. Vogt I-PpoI, the Endonuclease Encoded by the Group I Intron PpLSU3, Is Expressed from an RNA Polymerase I Transcript Mol. Cell. Biol., October 1, 1998; 18(10): 5809 - 5817. [Abstract] [Full Text]