HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ohta, T. Articles by Hirose, S. Search for Related Content PubMed PubMed Citation Articles by Ohta, T. Articles by Hirose, S. Social Bookmarking                      What's this?

Volume 270, Number 26, Issue of June 30, pp. 15571-15575, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Cloning of a cDNA for DNA Supercoiling Factor Reveals a Distinctive Ca-binding Protein

Tsutomu Ohta , Masatomo Kobayashi , Susumu Hirose

DNA supercoiling factor is a protein capable of generating negative supercoils into a relaxed DNA in conjunction with eukaryotic DNA topoisomerase II. A cDNA clone encoding the Bombyx mori supercoiling factor has been isolated and characterized. The cDNA sequence contains an open reading frame of 322 amino acids rich in acidic residues. A local region of the predicted polypeptide shares a weak homology with the central portion of bacterial DNA gyrase A subunit. The cDNA sequence also predicts two direct repeats within the factor. Each repeat unit consists of a potential EF-hand domain. The factor contains three other potential EF-hand domains and actually binds Ca. Moreover, the supercoiling reaction has been shown to be activated by Ca. These results implicate Ca in the control of the superhelical state of DNA.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				Y. Ogasawara, H. Furuhashi, and S. Hirose DNA supercoiling factor positively regulates expression of the homeotic gene Abdominal-B in Drosophila melanogaster. Genes Cells, December 1, 2007; 12(12): 1347 - 1355. [Abstract] [Full Text] [PDF]

				K. Lehmann, B. Hause, D. Altmann, and M. Kock Tomato Ribonuclease LX with the Functional Endoplasmic Reticulum Retention Motif HDEF Is Expressed during Programmed Cell Death Processes, Including Xylem Differentiation, Germination, and Senescence Plant Physiology, October 1, 2001; 127(2): 436 - 449. [Abstract] [Full Text] [PDF]

				M. Kobayashi, N. Aita, S. Hayashi, K. Okada, T. Ohta, and S. Hirose DNA Supercoiling Factor Localizes to Puffs on Polytene Chromosomes in Drosophila melanogaster Mol. Cell. Biol., November 1, 1998; 18(11): 6737 - 6744. [Abstract] [Full Text]

				D. Yabe, T. Nakamura, N. Kanazawa, K. Tashiro, and T. Honjo Calumenin, a Ca2+-binding Protein Retained in the Endoplasmic Reticulum with a Novel Carboxyl-terminal Sequence, HDEF J. Biol. Chem., July 18, 1997; 272(29): 18232 - 18239. [Abstract] [Full Text] [PDF]

				K.-i. Takemaru, F.-Q. Li, H. Ueda, and S. Hirose Multiprotein bridging factor 1 (MBF1) is an evolutionarily conserved transcriptional coactivator that connects a regulatory factor and TATA element-binding protein PNAS, July 8, 1997; 94(14): 7251 - 7256. [Abstract] [Full Text] [PDF]