A 24-kDa protein isolated by preparative gel electrophoresis from rat testes was reported by us as an active immunogen in rats. Anti-24-kDa antibodies inhibited murine sperm-oocyte binding in vitro. Here, we show similarity at the NH terminus shared by this protein purified on Sephadex G-75 followed by anion exchange high performance liquid chromatography with glutathione S-transferase (GST)-µ subunits. This protein purified by glutathione affinity chromatography also demonstrated similarity to GST-µ NH terminus in a 30-amino-acid overlap. Both proteins showed activity toward the GST substrate 1-chloro-2,4-dinitrobenzene (K of 33 µM and 50 µM) which was inhibited by 17-estradiol 3-sulfate. Antisera against both proteins recognized liver GST-µ on Western blots and sperm acrosome of multiple species immunocytochemically. Both antisera significantly inhibited in vitro fertilization of goat oocytes by sperm preincubated with them while anti-liver GST sera did not. GST activity was localized on rat sperm, seminiferous tubular fluid, and Sertoli cells. Seminiferous tubular fluid 24-kDa protein shared similarity to the NH terminus of GST-µ subunits in a 20-amino-acid overlap. Time-dependent accumulation of GST was detected in the spent culture medium of seminiferous tubules from rats of different ages suggesting secretion.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				T. R. Kumar and Muralidhara Induction of Oxidative Stress by Organic Hydroperoxides in Testis and Epididymal Sperm of Rats In Vivo J Androl, January 1, 2007; 28(1): 77 - 85. [Abstract] [Full Text] [PDF]

				D. P. Mishra and C. Shaha Estrogen-induced Spermatogenic Cell Apoptosis Occurs via the Mitochondrial Pathway: ROLE OF SUPEROXIDE AND NITRIC OXIDE J. Biol. Chem., February 18, 2005; 280(7): 6181 - 6196. [Abstract] [Full Text] [PDF]

				L. Benbrahim-Tallaa, E. Tabone, G. Tosser-Klopp, F. Hatey, and M. Benahmed Glutathione S-Transferase Alpha Expressed in Porcine Sertoli Cells Is under the Control of Follicle-Stimulating Hormone and Testosterone Biol Reprod, June 1, 2002; 66(6): 1734 - 1742. [Abstract] [Full Text] [PDF]

				T. Hemachand, B. Gopalakrishnan, D. M. Salunke, S. M. Totey, and C. Shaha Sperm plasma-membrane-associated glutathione S-transferases as gamete recognition molecules J. Cell Sci., May 15, 2002; 115(10): 2053 - 2065. [Abstract] [Full Text] [PDF]

				F. Remoue, J.-C. Mani, M. Pugniere, A.-M. Schacht, A. Capron, and G. Riveau Functional Specific Binding of Testosterone to Schistosoma haematobium 28-Kilodalton Glutathione S-Transferase Infect. Immun., February 1, 2002; 70(2): 601 - 605. [Abstract] [Full Text] [PDF]

				M. Das, S. B. Mukherjee, and C. Shaha Hydrogen peroxide induces apoptosis-like death in Leishmania donovani promastigotes J. Cell Sci., January 7, 2001; 114(13): 2461 - 2469. [Abstract] [Full Text] [PDF]