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Volume 270, Number 26, Issue of June 30, pp. 15815-15820, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
A cis-Acting Element in Rous Sarcoma Virus Long Terminal Repeat Required for Promoter Repression by HeLa Nuclear Protein p21

Chen-Hsiung Yeh , Aaron J. Shatkin

HeLa cell basic nuclear protein (p21), which represses Rous sarcoma virus long terminal repeat (RSV LTR) promoter activity, diminished v-src expression and the appearance at permissive temperature of the transformed phenotype in tsRSVLA23 Rat-1, a cell line transformed with a temperature-sensitive mutant of RSV. Nuclear run-on analyses using COS-1 cells cotransfected with p21 cDNA and chloramphenicol acetyltransferase reporter indicated that p21 inhibits transcription initiation by targeting a region in the RSV LTR promoter between positions -108 and -85 upstream of the cap site. Insertion of this 24-base pair sequence in place of one of the 72-base pair enhancers in the SV40 early promoter rendered it sensitive to p21 repression. Electrophoretic mobility shift assays using a synthetic oligomer corresponding to the 24-base pair LTR promoter element revealed that p21 altered the pattern of proteinDNA complex formation apparently without binding DNA directly. Complex formation assayed by UV cross-linking and DNA affinity chromatography indicated further that a cellular factor which can interact with this element was decreased in cells transfected with p21 expression plasmid. The results indicate that p21 repression of RSV LTR is mediated by a cis-acting element and may occur by alteration of protein complexes formed on this promoter element.



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C.-H. Yeh, W.-X. Zong, and A. J. Shatkin
The Ser[IMAGE]-Ser[IMAGE] Pair in HeLa Nuclear Protein p21/SIIR Mediates Ser/Thr Phosphorylation and Is Essential for Rous Sarcoma Virus Long Terminal Repeat Repression
J. Biol. Chem., October 27, 1995; 270(43): 25313 - 25315.
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Copyright © 1995 by the American Society for Biochemistry and Molecular Biology.