| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The voltage-dependent anion-selective channel (VDAC) in
mitochondrial outer membranes is formed by a polypeptide (M
Volume 270,
Number 28,
Issue of July 14, pp. 16694-16700, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
31,000) coded by a nuclear gene whose cDNA
sequence is known for several organisms. Antibodies have been raised
against synthetic peptides corresponding to four different regions in
the predicted sequence of the VDAC polypeptide of the fungus Neurospora crassa (residues 1-20, amino terminus;
195-210, 251-268, and 272-283, carboxyl terminus).
Specificity of the antibodies has been characterized in terms of
binding to peptides or fungal mitochondria on microtiter plates and
binding to mitochondrial proteins of several species in Western blots.
Reactivity of three of the four antibodies with fungal mitochondria in
suspension increases with lysis of outer membranes, indicating that the
respective epitopes (including those near the amino and carboxyl
termini) are exposed on the surface of the outer membrane that faces
inside the mitochondrion. Preincubation of mitochondria with a
polyanion that modulates VDAC voltage dependence strongly inhibits
binding of the antibody against residues 251-268, whose epitopes
are on the outer mitochondrial surface.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Ujwal, D. Cascio, J.-P. Colletier, S. Faham, J. Zhang, L. Toro, P. Ping, and J. Abramson The crystal structure of mouse VDAC1 at 2.3 A resolution reveals mechanistic insights into metabolite gating PNAS, November 18, 2008; 105(46): 17742 - 17747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hiller, R. G. Garces, T. J. Malia, V. Y. Orekhov, M. Colombini, and G. Wagner Solution Structure of the Integral Human Membrane Protein VDAC-1 in Detergent Micelles Science, August 29, 2008; 321(5893): 1206 - 1210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Runke, E. Maier, W. A. T. Summers, D. C. Bay, R. Benz, and D. A. Court Deletion Variants of Neurospora Mitochondrial Porin: Electrophysiological and Spectroscopic Analysis Biophys. J., May 1, 2006; 90(9): 3155 - 3164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nakamura, H. Suzuki, M. Sakaguchi, and K. Mihara Targeting and Assembly of Rat Mitochondrial Translocase of Outer Membrane 22 (TOM22) into the TOM Complex J. Biol. Chem., May 14, 2004; 279(20): 21223 - 21232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Horie, H. Suzuki, M. Sakaguchi, and K. Mihara Characterization of Signal That Directs C-Tail-anchored Proteins to Mammalian Mitochondrial Outer Membrane Mol. Biol. Cell, May 1, 2002; 13(5): 1615 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Jacotot, K. F. Ferri, C. El Hamel, C. Brenner, S. Druillennec, J. Hoebeke, P. Rustin, D. Metivier, C. Lenoir, M. Geuskens, et al. Control of Mitochondrial Membrane Permeabilization by Adenine Nucleotide Translocator Interacting with HIV-1 Viral Protein R and Bcl-2 J. Exp. Med., February 20, 2001; 193(4): 509 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kanaji, J. Iwahashi, Y. Kida, M. Sakaguchi, and K. Mihara Characterization of the Signal that Directs Tom20 to the Mitochondrial Outer Membrane J. Cell Biol., October 18, 2000; 151(2): 277 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Schleiff, J. R. Silvius, and G. C. Shore Direct Membrane Insertion of Voltage-dependent Anion-selective Channel Protein Catalyzed by Mitochondrial Tom20 J. Cell Biol., May 31, 1999; 145(5): 973 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Song, C. Midson, E. Blachly-Dyson, M. Forte, and M. Colombini The Topology of VDAC as Probed by Biotin Modification J. Biol. Chem., September 18, 1998; 273(38): 24406 - 24413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Koppel, K. W. Kinnally, P. Masters, M. Forte, E. Blachly-Dyson, and C. A. Mannella Bacterial Expression and Characterization of the Mitochondrial Outer Membrane Channel. EFFECTS OF N-TERMINAL MODIFICATIONS J. Biol. Chem., May 29, 1998; 273(22): 13794 - 13800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Joseph-Liauzun, P. Delmas, D. Shire, and P. Ferrara Topological Analysis of the Peripheral Benzodiazepine Receptor in Yeast Mitochondrial Membranes Supports a Five-transmembrane Structure J. Biol. Chem., January 23, 1998; 273(4): 2146 - 2152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Popp, D. A. Court, R. Benz, W. Neupert, and R. Lill The Role of the N and C Termini of Recombinant Neurospora Mitochondrial Porin in Channel Formation and Voltage-dependent Gating J. Biol. Chem., June 7, 1996; 271(23): 13593 - 13599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. V. Pavlov, M. Priault, D. Pietkiewicz, E. H.-Y. Cheng, B. Antonsson, S. Manon, S. J. Korsmeyer, C. A. Mannella, and K. W. Kinnally A novel, high conductance channel of mitochondria linked to apoptosis in mammalian cells and Bax expression in yeast J. Cell Biol., November 26, 2001; 155(5): 725 - 732. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
