| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Cytochrome b of human neutrophils is the central
component of the microbicidal NADPH-oxidase system. However, the
folding topology of this integral membrane protein remains
undetermined. Two random-sequence bacteriophage peptide libraries were
used to map structural features of cytochrome b by determining
the epitopes of monoclonal antibodies (mAbs) 44.1 and 54.1, specific
for the p22
Volume 270,
Number 28,
Issue of July 14, pp. 16974-16980, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
and gp91
cytochrome b chains, respectively. The unique
peptides of phage selected by mAb affinity purification were deduced
from the phage DNA sequences. Phage selected by mAb 44.1 displayed the
consensus peptide sequence GGPQVXPI, which is nearly identical
to
GGPQVNPI
of p22
.
Phage selected by mAb 54.1 displayed the consensus sequence
PKXAVDGP, which resembles
PKIAVDGP
of gp91
. Western blotting demonstrated
specific binding of each mAb to the respective cytochrome b subunit and selected phage peptides. In flow cytometric analysis,
mAb 44.1 bound only permeabilized neutrophils, while 54.1 did not bind
intact or permeabilized cells. However, mAb 54.1 immunosedimented
detergent-solubilized cytochrome b in sucrose gradients. These
results suggest the
GGPQVNPI
segment of
p22
is accessible on its intracellular surface,
but the
PKIAVDGP
region on
gp91
is not accessible to antibody, and
probably not on the protein surface.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. A. Gauss, L. K. Nelson-Overton, D. W. Siemsen, Y. Gao, F. R. DeLeo, and M. T. Quinn Role of NF-{kappa}B in transcriptional regulation of the phagocyte NADPH oxidase by tumor necrosis factor-{alpha} J. Leukoc. Biol., September 1, 2007; 82(3): 729 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Riesselman, H. M. Miettinen, J. M. Gripentrog, C. I. Lord, B. Mumey, E. A. Dratz, J. Stie, R. M. Taylor, and A. J. Jesaitis C-Terminal Tail Phosphorylation of N-Formyl Peptide Receptor: Differential Recognition of Two Neutrophil Chemoattractant Receptors by Monoclonal Antibodies NFPR1 and NFPR2 J. Immunol., August 15, 2007; 179(4): 2520 - 2531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Stie, A. V. Jesaitis, C. I. Lord, J. M. Gripentrog, R. M. Taylor, J. B. Burritt, and A. J. Jesaitis Localization of hCAP-18 on the surface of chemoattractant-stimulated human granulocytes: analysis using two novel hCAP-18-specific monoclonal antibodies J. Leukoc. Biol., July 1, 2007; 82(1): 161 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. H. Schulman, M.-S. Zhou, E. A. Jaimes, and L. Raij Dissociation between metabolic and vascular insulin resistance in aging Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H853 - H859. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Mayrose, T. Shlomi, N. D. Rubinstein, J. M. Gershoni, E. Ruppin, R. Sharan, and T. Pupko Epitope mapping using combinatorial phage-display libraries: a graph-based algorithm Nucleic Acids Res., January 12, 2007; 35(1): 69 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. McCaffrey and L.-A. H. Allen Francisella tularensis LVS evades killing by human neutrophils via inhibition of the respiratory burst and phagosome escape J. Leukoc. Biol., December 1, 2006; 80(6): 1224 - 1230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhu, C. C. Marchal, A.-J. Casbon, N. Stull, K. von Lohneysen, U. G. Knaus, A. J. Jesaitis, S. McCormick, W. M. Nauseef, and M. C. Dinauer Deletion Mutagenesis of p22phox Subunit of Flavocytochrome b558: IDENTIFICATION OF REGIONS CRITICAL FOR gp91phox MATURATION AND NADPH OXIDASE ACTIVITY J. Biol. Chem., October 13, 2006; 281(41): 30336 - 30346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hidalgo, G. Sanchez, G. Barrientos, and P. Aracena-Parks A Transverse Tubule NADPH Oxidase Activity Stimulates Calcium Release from Isolated Triads via Ryanodine Receptor Type 1 S -Glutathionylation J. Biol. Chem., September 8, 2006; 281(36): 26473 - 26482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Moreland, A. P. Davis, G. Bailey, W. M. Nauseef, and Fred. S. Lamb Anion Channels, Including ClC-3, Are Required for Normal Neutrophil Oxidative Function, Phagocytosis, and Transendothelial Migration J. Biol. Chem., May 5, 2006; 281(18): 12277 - 12288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Moreau, C. Granier, S. Villard, D. Laune, and F. Molina Discontinuous epitope prediction based on mimotope analysis Bioinformatics, May 1, 2006; 22(9): 1088 - 1095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Burlak, A. R. Whitney, D. J. Mead, T. Hackstadt, and F. R. DeLeo Maturation of Human Neutrophil Phagosomes Includes Incorporation of Molecular Chaperones and Endoplasmic Reticulum Quality Control Machinery Mol. Cell. Proteomics, April 1, 2006; 5(4): 620 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kondo, M. Shimizu, M. Urushihara, K. Tsuchiya, M. Yoshizumi, T. Tamaki, A. Nishiyama, H. Kawachi, F. Shimizu, M. T. Quinn, et al. Addition of the Antioxidant Probucol to Angiotensin II Type I Receptor Antagonist Arrests Progressive Mesangioproliferative Glomerulonephritis in the Rat J. Am. Soc. Nephrol., March 1, 2006; 17(3): 783 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Li, M. M. Harraz, W. Zhou, L. N. Zhang, W. Ding, Y. Zhang, T. Eggleston, C. Yeaman, B. Banfi, and J. F. Engelhardt Nox2 and Rac1 Regulate H2O2-Dependent Recruitment of TRAF6 to Endosomal Interleukin-1 Receptor Complexes Mol. Cell. Biol., January 1, 2006; 26(1): 140 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Yang, L. D. Asico, P. Yu, Z. Wang, J. E. Jones, C. S. Escano, X. Wang, M. T. Quinn, D. R. Sibley, G. G. Romero, et al. D5 dopamine receptor regulation of reactive oxygen species production, NADPH oxidase, and blood pressure Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R96 - R104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N.-K. Pham, J. Mouriz, and P. E. Kima Leishmania pifanoi Amastigotes Avoid Macrophage Production of Superoxide by Inducing Heme Degradation Infect. Immun., December 1, 2005; 73(12): 8322 - 8333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Martyn, M.-J. Kim, M. T. Quinn, M. C. Dinauer, and U. G. Knaus p21-activated kinase (Pak) regulates NADPH oxidase activation in human neutrophils Blood, December 1, 2005; 106(12): 3962 - 3969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. Sheppard, M. R. Kelher, E. E. Moore, N. J. D. McLaughlin, A. Banerjee, and C. C. Silliman Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation J. Leukoc. Biol., November 1, 2005; 78(5): 1025 - 1042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Beckmann, M. Brittnacher, R. Ernst, N. Mayer-Hamblett, S. I. Miller, and J. L. Burns Use of Phage Display To Identify Potential Pseudomonas aeruginosa Gene Products Relevant to Early Cystic Fibrosis Airway Infections Infect. Immun., January 1, 2005; 73(1): 444 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Taylor, J. B. Burritt, D. Baniulis, T. R. Foubert, C. I. Lord, M. C. Dinauer, C. A. Parkos, and A. J. Jesaitis Site-Specific Inhibitors of NADPH Oxidase Activity and Structural Probes of Flavocytochrome b: Characterization of Six Monoclonal Antibodies to the p22phox Subunit J. Immunol., December 15, 2004; 173(12): 7349 - 7357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. IJdo and A. C. Mueller Neutrophil NADPH Oxidase Is Reduced at the Anaplasma phagocytophilum Phagosome Infect. Immun., September 1, 2004; 72(9): 5392 - 5401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maddaloni, C. Cooke, R. Wilkinson, A. V. Stout, L. Eng, and S. H. Pincus Immunological Characteristics Associated with the Protective Efficacy of Antibodies to Ricin J. Immunol., May 15, 2004; 172(10): 6221 - 6228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Mandell, I. C. McCall, and C. A. Parkos Involvement of the Junctional Adhesion Molecule-1 (JAM1) Homodimer Interface in Regulation of Epithelial Barrier Function J. Biol. Chem., April 16, 2004; 279(16): 16254 - 16262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, M. B. O'Connor, K. J. Mandell, K. Zen, A. Ullrich, H.-J. Buhring, and C. A. Parkos Peptide-Mediated Inhibition of Neutrophil Transmigration by Blocking CD47 Interactions with Signal Regulatory Protein {alpha} J. Immunol., February 15, 2004; 172(4): 2578 - 2585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. W. Bailey, B. Mumey, P. A. Hargrave, A. Arendt, O. P. Ernst, K. P. Hofmann, P. R. Callis, J. B. Burritt, A. J. Jesaitis, and E. A. Dratz Constraints on the conformation of the cytoplasmic face of dark-adapted and light-excited rhodopsin inferred from antirhodopsin antibody imprints Protein Sci., November 1, 2003; 12(11): 2453 - 2475. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Brar, Z. Corbin, T. P. Kennedy, R. Hemendinger, L. Thornton, B. Bommarius, R. S. Arnold, A. R. Whorton, A. B. Sturrock, T. P. Huecksteadt, et al. NOX5 NAD(P)H oxidase regulates growth and apoptosis in DU 145 prostate cancer cells Am J Physiol Cell Physiol, August 1, 2003; 285(2): C353 - C369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Villard, S. Lacroix-Desmazes, T. Kieber-Emmons, D. Piquer, S. Grailly, A. Benhida, S. V. Kaveri, J.-M. Saint-Remy, and C. Granier Peptide decoys selected by phage display block in vitro and in vivo activity of a human anti-FVIII inhibitor Blood, August 1, 2003; 102(3): 949 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. C. Gerling, Y. Sun, R. A. Ahokas, L. A. Wodi, S. K. Bhattacharya, K. J. Warrington, A. E. Postlethwaite, and K. T. Weber Aldosteronism: an immunostimulatory state precedes proinflammatory/fibrogenic cardiac phenotype Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H813 - H821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Burritt, T. R. Foubert, D. Baniulis, C. I. Lord, R. M. Taylor, J. S. Mills, T. D. Baughan, D. Roos, C. A. Parkos, and A. J. Jesaitis Functional Epitope on Human Neutrophil Flavocytochrome b558 J. Immunol., June 15, 2003; 170(12): 6082 - 6089. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.M. Touyz, G. Yao, and E.L. Schiffrin c-Src Induces Phosphorylation and Translocation of p47phox: Role in Superoxide Generation by Angiotensin II in Human Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., June 1, 2003; 23(6): 981 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Jaye, F. S. Nolte, L. Mazzucchelli, C. Geigerman, A. Akyildiz, and C. A. Parkos Use of Real-Time Polymerase Chain Reaction to Identify Cell- and Tissue-Type-Selective Peptides by Phage Display Am. J. Pathol., May 1, 2003; 162(5): 1419 - 1429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ushio-Fukai, Y. Tang, T. Fukai, S. I. Dikalov, Y. Ma, M. Fujimoto, M. T. Quinn, P. J. Pagano, C. Johnson, and R. W. Alexander Novel Role of gp91phox-Containing NAD(P)H Oxidase in Vascular Endothelial Growth Factor-Induced Signaling and Angiogenesis Circ. Res., December 13, 2002; 91(12): 1160 - 1167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kalinina, A. Agrotis, E. Tararak, Y. Antropova, P. Kanellakis, O. Ilyinskaya, M. T. Quinn, V. Smirnov, and A. Bobik Cytochrome b558-Dependent NAD(P)H Oxidase-Phox Units in Smooth Muscle and Macrophages of Atherosclerotic Lesions Arterioscler. Thromb. Vasc. Biol., December 1, 2002; 22(12): 2037 - 2043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sun, J. Zhang, L. Lu, S. S. Chen, M. T. Quinn, and K. T. Weber Aldosterone-Induced Inflammation in the Rat Heart : Role of Oxidative Stress Am. J. Pathol., November 1, 2002; 161(5): 1773 - 1781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Touyz, X. Chen, F. Tabet, G. Yao, G. He, M. T. Quinn, P. J. Pagano, and E. L. Schiffrin Expression of a Functionally Active gp91phox-Containing Neutrophil-Type NAD(P)H Oxidase in Smooth Muscle Cells From Human Resistance Arteries: Regulation by Angiotensin II Circ. Res., June 14, 2002; 90(11): 1205 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M.-C. Dang, A. R. Cross, M. T. Quinn, and B. M. Babior Assembly of the neutrophil respiratory burst oxidase: A direct interaction between p67PHOX and cytochrome b558 II PNAS, April 2, 2002; 99(7): 4262 - 4265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mott, Y. Rikihisa, and S. Tsunawaki Effects of Anaplasma phagocytophila on NADPH Oxidase Components in Human Neutrophils and HL-60 Cells Infect. Immun., March 1, 2002; 70(3): 1359 - 1366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Dahan, I. Issaeva, Y. Gorzalczany, N. Sigal, M. Hirshberg, and E. Pick Mapping of Functional Domains in the p22phox Subunit of Flavocytochrome b559 Participating in the Assembly of the NADPH Oxidase Complex by "Peptide Walking" J. Biol. Chem., March 1, 2002; 277(10): 8421 - 8432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Gauss, P. L. Mascolo, D. W. Siemsen, L. K. Nelson, P. L. Bunger, P. J. Pagano, and M. T. Quinn Cloning and sequencing of rabbit leukocyte NADPH oxidase genes reveals a unique p67phox homolog J. Leukoc. Biol., February 1, 2002; 71(2): 319 - 328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Chabrashvili, A. Tojo, M. L. Onozato, C. Kitiyakara, M. T. Quinn, T. Fujita, W. J. Welch, and C. S. Wilcox Expression and Cellular Localization of Classic NADPH Oxidase Subunits in the Spontaneously Hypertensive Rat Kidney Hypertension, February 1, 2002; 39(2): 269 - 274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Foubert, J. B. Bleazard, J. B. Burritt, J. M. Gripentrog, D. Baniulis, R. M. Taylor, and A. J. Jesaitis Identification of a Spectrally Stable Proteolytic Fragment of Human Neutrophil Flavocytochrome b Composed of the NH2-terminal Regions of gp91phox and p22phox J. Biol. Chem., October 12, 2001; 276(42): 38852 - 38861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. WAGNER, M. R. SCHROETER, and M. HECKER 17{beta}-Estradiol inhibition of NADPH oxidase expression in human endothelial cells FASEB J, October 1, 2001; 15(12): 2121 - 2130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Jaye, H. A. Edens, L. Mazzucchelli, and C. A. Parkos Novel G Protein-Coupled Responses in Leukocytes Elicited by a Chemotactic Bacteriophage Displaying a Cell Type-Selective Binding Peptide J. Immunol., June 15, 2001; 166(12): 7250 - 7259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gallois, J. R. Klein, L.-A. H. Allen, B. D. Jones, and W. M. Nauseef Salmonella Pathogenicity Island 2-Encoded Type III Secretion System Mediates Exclusion of NADPH Oxidase Assembly from the Phagosomal Membrane J. Immunol., May 1, 2001; 166(9): 5741 - 5748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Xia, B. Anderson, Q. Mao, and B. L. Davidson Recombinant Human Adenovirus: Targeting to the Human Transferrin Receptor Improves Gene Transfer to Brain Microcapillary Endothelium J. Virol., December 1, 2000; 74(23): 11359 - 11366. [Abstract] [Full Text]
|
|
|
|
|
|
M. E. Cifuentes, F. E. Rey, O. A. Carretero, and P. J. Pagano Upregulation of p67phox and gp91phox in aortas from angiotensin II-infused mice Am J Physiol Heart Circ Physiol, November 1, 2000; 279(5): H2234 - H2240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. DeLeo, J. B. Burritt, L. Yu, A. J. Jesaitis, M. C. Dinauer, and W. M. Nauseef Processing and Maturation of Flavocytochrome b558 Include Incorporation of Heme as a Prerequisite for Heterodimer Assembly J. Biol. Chem., April 28, 2000; 275(18): 13986 - 13993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Regier, K. A. Waite, R. Wallin, and L. C. McPhail A Phosphatidic Acid-activated Protein Kinase and Conventional Protein Kinase C Isoforms Phosphorylate p22phox, an NADPH Oxidase Component J. Biol. Chem., December 17, 1999; 274(51): 36601 - 36608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Glee, S. H. Pincus, D. K. McNamer, M. J. Smith, J. B. Burritt, and J. E. Cutler Peptide Ligands That Bind IgM Antibodies and Block Interaction with Antigen J. Immunol., July 15, 1999; 163(2): 826 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-A. H. Allen, F. R. DeLeo, A. Gallois, S. Toyoshima, K. Suzuki, and W. M. Nauseef Transient Association of the Nicotinamide Adenine Dinucleotide Phosphate Oxidase Subunits p47phox and p67phox With Phagosomes in Neutrophils From Patients With X-Linked Chronic Granulomatous Disease Blood, May 15, 1999; 93(10): 3521 - 3530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Wang, S. Hope, Y. Du, M. T. Quinn, A. Cayatte, P. J. Pagano, and R. A. Cohen Paracrine Role of Adventitial Superoxide Anion in Mediating Spontaneous Tone of the Isolated Rat Aorta in Angiotensin II-Induced Hypertension Hypertension, May 1, 1999; 33(5): 1225 - 1232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Mazzucchelli, J. B. Burritt, A. J. Jesaitis, A. Nusrat, T. W. Liang, A. T. Gewirtz, F. J. Schnell, and C. A. Parkos Cell-Specific Peptide Binding by Human Neutrophils Blood, March 1, 1999; 93(5): 1738 - 1748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Han, R. P. Morrison, and J. E. Cutler A Vaccine and Monoclonal Antibodies That Enhance Mouse Resistance to Candida albicans Vaginal Infection Infect. Immun., December 1, 1998; 66(12): 5771 - 5776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Burritt, S. C. Busse, D. Gizachew, D. W. Siemsen, M. T. Quinn, C. W. Bond, E. A. Dratz, and A. J. Jesaitis Antibody Imprint of a Membrane Protein Surface. PHAGOCYTE FLAVOCYTOCHROME b J. Biol. Chem., September 18, 1998; 273(38): 24847 - 24852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Di Wang, P. J. Pagano, Y. Du, A. J. Cayatte, M. T. Quinn, P. Brecher, and R. A. Cohen Superoxide Anion From the Adventitia of the Rat Thoracic Aorta Inactivates Nitric Oxide Circ. Res., April 20, 1998; 82(7): 810 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Pincus, M. J. Smith, H. J. Jennings, J. B. Burritt, and P. M. Glee Peptides That Mimic the Group B Streptococcal Type III Capsular Polysaccharide Antigen J. Immunol., January 1, 1998; 160(1): 293 - 298. [Abstract] |
