| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, March 27, 1995; and in revised form, April 6, 1995) From the Analysis of potentiometric titrations of the cytochrome b
Volume 270,
Number 29,
Issue of July 21, pp. 17075-17077, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
of the Neutrophil Superoxide-generating
System Contains Two Nonidentical Hemes
POTENTIOMETRIC STUDIES OF A MUTANT FORM OF
gp91
from a X
chronic
granulomatous disease patient with an Arg
Ser
mutation in gp91
indicates that the mutant form
of the cytochrome contains two nonidentical hemes with midpoint
potentials of E
= -220 and E
= -300 mV. In the light
of this information, reanalysis of redox titrations of wild-type
cytochrome b
implies that it probably
also contains two separate heme centers with midpoint potentials of E
= -225 and E
= -265 mV. The effect of
the Arg
Ser substitution is to reduce the midpoint
potential of one of the heme centers by approximately 35 mV and
suggests possible interaction between Arg
and a heme
propionate side chain.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  W. M. Nauseef Biological Roles for the NOX Family NADPH Oxidases J. Biol. Chem., June 20, 2008; 283(25): 16961 - 16965. [Full Text] [PDF]
|
|
|
|
 |
|
 X. J. Li, F. Fieschi, M.-H. Paclet, D. Grunwald, Y. Campion, P. Gaudin, F. Morel, and M.-J. Stasia Leu505 of Nox2 is crucial for optimal p67phox-dependent activation of the flavocytochrome b558 during phagocytic NADPH oxidase assembly J. Leukoc. Biol., January 1, 2007; 81(1): 238 - 249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhu, C. C. Marchal, A.-J. Casbon, N. Stull, K. von Lohneysen, U. G. Knaus, A. J. Jesaitis, S. McCormick, W. M. Nauseef, and M. C. Dinauer Deletion Mutagenesis of p22phox Subunit of Flavocytochrome b558: IDENTIFICATION OF REGIONS CRITICAL FOR gp91phox MATURATION AND NADPH OXIDASE ACTIVITY J. Biol. Chem., October 13, 2006; 281(41): 30336 - 30346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. Sheppard, M. R. Kelher, E. E. Moore, N. J. D. McLaughlin, A. Banerjee, and C. C. Silliman Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation J. Leukoc. Biol., November 1, 2005; 78(5): 1025 - 1042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. J. Li, D. Grunwald, J. Mathieu, F. Morel, and M.-J. Stasia Crucial Role of Two Potential Cytosolic Regions of Nox2, 191TSSTKTIRRS200 and 484DESQANHFAVHHDEEKD500, on NADPH Oxidase Activation J. Biol. Chem., April 15, 2005; 280(15): 14962 - 14973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Taylor, J. B. Burritt, D. Baniulis, T. R. Foubert, C. I. Lord, M. C. Dinauer, C. A. Parkos, and A. J. Jesaitis Site-Specific Inhibitors of NADPH Oxidase Activity and Structural Probes of Flavocytochrome b: Characterization of Six Monoclonal Antibodies to the p22phox Subunit J. Immunol., December 15, 2004; 173(12): 7349 - 7357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Quinn and K. A. Gauss Structure and regulation of the neutrophil respiratory burst oxidase: comparison with nonphagocyte oxidases J. Leukoc. Biol., October 1, 2004; 76(4): 760 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhu, K. Larade, T. A. Jackson, J. Xie, A. Ladoux, H. Acker, U. Berchner-Pfannschmidt, J. Fandrey, A. R. Cross, G. S. Lukat-Rodgers, et al. NCB5OR Is a Novel Soluble NAD(P)H Reductase Localized in the Endoplasmic Reticulum J. Biol. Chem., July 16, 2004; 279(29): 30316 - 30325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hashida, S. Yuzawa, N. N. Suzuki, Y. Fujioka, T. Takikawa, H. Sumimoto, F. Inagaki, and H. Fujii Binding of FAD to Cytochrome b558 Is Facilitated during Activation of the Phagocyte NADPH Oxidase, Leading to Superoxide Production J. Biol. Chem., June 18, 2004; 279(25): 26378 - 26386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mahadev, H. Motoshima, X. Wu, J. M. Ruddy, R. S. Arnold, G. Cheng, J. D. Lambeth, and B. J. Goldstein The NAD(P)H Oxidase Homolog Nox4 Modulates Insulin-Stimulated Generation of H2O2 and Plays an Integral Role in Insulin Signal Transduction Mol. Cell. Biol., March 1, 2004; 24(5): 1844 - 1854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Clark, M. J. Coffey, R. M. Maclean, P. W. Collins, M. J. Lewis, A. R. Cross, and V. B. O'Donnell Characterization of Nitric Oxide Consumption Pathways by Normal, Chronic Granulomatous Disease and Myeloperoxidase-Deficient Human Neutrophils J. Immunol., November 15, 2002; 169(10): 5889 - 5896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Biberstine-Kinkade, L. Yu, N. Stull, B. LeRoy, S. Bennett, A. Cross, and M. C. Dinauer Mutagenesis of p22phox Histidine 94. A HISTIDINE IN THIS POSITION IS NOT REQUIRED FOR FLAVOCYTOCHROME b558 FUNCTION J. Biol. Chem., August 9, 2002; 277(33): 30368 - 30374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. A. Edens, L. Sharling, G. Cheng, R. Shapira, J. M. Kinkade, T. Lee, H. A. Edens, X. Tang, C. Sullards, D. B. Flaherty, et al. Tyrosine cross-linking of extracellular matrix is catalyzed by Duox, a multidomain oxidase/peroxidase with homology to the phagocyte oxidase subunit gp91phox J. Cell Biol., August 20, 2001; 154(4): 879 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. DeLeo, J. B. Burritt, L. Yu, A. J. Jesaitis, M. C. Dinauer, and W. M. Nauseef Processing and Maturation of Flavocytochrome b558 Include Incorporation of Heme as a Prerequisite for Heterodimer Assembly J. Biol. Chem., April 28, 2000; 275(18): 13986 - 13993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Regier, K. A. Waite, R. Wallin, and L. C. McPhail A Phosphatidic Acid-activated Protein Kinase and Conventional Protein Kinase C Isoforms Phosphorylate p22phox, an NADPH Oxidase Component J. Biol. Chem., December 17, 1999; 274(51): 36601 - 36608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nisimoto, S. Motalebi, C.-H. Han, and J. D. Lambeth The p67phox Activation Domain Regulates Electron Flow from NADPH to Flavin in Flavocytochrome b558 J. Biol. Chem., August 13, 1999; 274(33): 22999 - 23005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Cross, R. W. Erickson, and J. T. Curnutte Simultaneous Presence of p47phox and Flavocytochrome b-245 Are Required for the Activation of NADPH Oxidase by Anionic Amphiphiles. EVIDENCE FOR AN INTERMEDIATE STATE OF OXIDASE ACTIVATION J. Biol. Chem., May 28, 1999; 274(22): 15519 - 15525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Babior NADPH Oxidase: An Update Blood, March 1, 1999; 93(5): 1464 - 1476. [Full Text] [PDF]
|
|
|
|
|
|
L. M. Henderson Role of Histidines Identified by Mutagenesis in the NADPH Oxidase-associated H+ Channel J. Biol. Chem., December 11, 1998; 273(50): 33216 - 33223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Yoshida, F. Saruta, K. Yoshikawa, O. Tatsuzawa, and S. Tsunawaki Mutation at Histidine 338 of gp91phox Depletes FAD and Affects Expression of Cytochrome b558 of the Human NADPH Oxidase J. Biol. Chem., October 23, 1998; 273(43): 27879 - 27886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Yu, M. T. Quinn, A. R. Cross, and M. C. Dinauer Gp91phox is the heme binding subunit of the superoxide-generating NADPH oxidase PNAS, July 7, 1998; 95(14): 7993 - 7998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhen, L. Yu, and M. C. Dinauer Probing the Role of the Carboxyl Terminus of the gp91phox Subunit of Neutrophil Flavocytochrome b558 using Site-directed Mutagenesis J. Biol. Chem., March 13, 1998; 273(11): 6575 - 6581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yu, L. Zhen, and M. C. Dinauer Biosynthesis of the Phagocyte NADPH Oxidase Cytochrome b558. ROLE OF HEME INCORPORATION AND HETERODIMER FORMATION IN MATURATION AND STABILITY OF gp91phox and p22phox SUBUNITS J. Biol. Chem., October 24, 1997; 272(43): 27288 - 27294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Finegold, K. P. Shatwell, A. W. Segal, R. D. Klausner, and A. Dancis Intramembrane Bis-Heme Motif for Transmembrane Electron Transport Conserved in a Yeast Iron Reductase and the Human NADPH Oxidase J. Biol. Chem., December 6, 1996; 271(49): 31021 - 31024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Shatwell, A. Dancis, A. R. Cross, R. D. Klausner, and A. W. Segal The FRE1 Ferric Reductase of Saccharomyces cerevisiae Is a Cytochrome b Similar to That of NADPH Oxidase J. Biol. Chem., June 14, 1996; 271(24): 14240 - 14244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Regier, D. G. Greene, S. Sergeant, A. J. Jesaitis, and L. C. McPhail Phosphorylation of p22phox Is Mediated by Phospholipase D-dependent and -independent Mechanisms. CORRELATION OF NADPH OXIDASE ACTIVITY AND p22phox PHOSPHORYLATION J. Biol. Chem., September 8, 2000; 275(37): 28406 - 28412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Biberstine-Kinkade, F. R. DeLeo, R. I. Epstein, B. A. LeRoy, W. M. Nauseef, and M. C. Dinauer Heme-ligating Histidines in Flavocytochrome b558. IDENTIFICATION OF SPECIFIC HISTIDINES IN gp91phox J. Biol. Chem., August 10, 2001; 276(33): 31105 - 31112. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
