The tachykinin substance P (SP) is a peptide transmitter of primary afferents. Its actions on both central and peripheral targets are mediated by a G-protein-coupled receptor of known primary structure. To identify contact sites between the undecapeptide SP and its receptor, we prepared radiolabeled photoreactive analogs of SP (H-RPKPQQFFGLM-NH) by replacing amino acids in the peptide with p-benzoyl-L-phenylalanine (BPA). SP, BPA-SP, and BPA-SP bind with high affinity (K

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