Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. Multimerin is composed of varying sized, disulfide-linked multimers, the smallest of which is a homotrimer. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. The cDNA for human multimerin was isolated from gt11 endothelial cell libraries using antibodies, and the isolated cDNA clones were used to obtain the full sequence. The full-length multimerin cDNA was 4.2 kilobase pairs. Northern analyses identified a 4.7-kilobase transcript in cultured endothelial cells, a megakaryocytic cell line, platelets, and highly vascular tissues. The multimerin cDNA can encode a protein of 1228 amino acids with the probable signal peptide cleavage site between amino acids 19 and 20. The protein is predicted to be hydrophilic and to contain 23 N-glycosylation sites. The adhesive motif RGDS (Arg-Gly-Asp-Ser) and an epidermal growth factor-like domain were identified. Sequence searches indicated that multimerin is a unique protein. Analyses identified probable coiled-coil structures in the central portion of the multimerin sequence. Additionally, the carboxyl-terminal region of multimerin resembles the globular, non-collagen-like, carboxylterminal domains of several other trimeric proteins, including complement C1q and collagens type VIII and X.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. B. Jeimy, R. A. Woram, N. Fuller, M. A. Quinn-Allen, G. A. F. Nicolaes, B. Dahlback, W. H. Kane, and C. P. M. Hayward Identification of the MMRN1 Binding Region within the C2 Domain of Human Factor V J. Biol. Chem., December 3, 2004; 279(49): 51466 - 51471. [Abstract] [Full Text] [PDF]

				M. Zanetti, P. Braghetta, P. Sabatelli, I. Mura, R. Doliana, A. Colombatti, D. Volpin, P. Bonaldo, and G. M. Bressan EMILIN-1 Deficiency Induces Elastogenesis and Vascular Cell Defects Mol. Cell. Biol., January 15, 2004; 24(2): 638 - 650. [Abstract] [Full Text] [PDF]

				W. W. Raymond, S. W. Ruggles, C. S. Craik, and G. H. Caughey Albumin Is a Substrate of Human Chymase: PREDICTION BY COMBINATORIAL PEPTIDE SCREENING AND DEVELOPMENT OF A SELECTIVE INHIBITOR BASED ON THE ALBUMIN CLEAVAGE SITE J. Biol. Chem., September 5, 2003; 278(36): 34517 - 34524. [Abstract] [Full Text] [PDF]

				U. Kishore, S. K. Gupta, M. V. Perdikoulis, M. S. Kojouharova, B. C. Urban, and K. B. M. Reid Modular Organization of the Carboxyl-Terminal, Globular Head Region of Human C1q A, B, and C Chains J. Immunol., July 15, 2003; 171(2): 812 - 820. [Abstract] [Full Text] [PDF]

				P. Spessotto, M. Cervi, M. T. Mucignat, G. Mungiguerra, I. Sartoretto, R. Doliana, and A. Colombatti beta 1 Integrin-dependent Cell Adhesion to EMILIN-1 Is Mediated by the gC1q Domain J. Biol. Chem., February 14, 2003; 278(8): 6160 - 6167. [Abstract] [Full Text] [PDF]

				K. G. Mann and M. Kalafatis Factor V: a combination of Dr Jekyll and Mr Hyde Blood, January 1, 2003; 101(1): 20 - 30. [Full Text] [PDF]

				S. Christian, H. Ahorn, M. Novatchkova, P. Garin-Chesa, J. E. Park, G. Weber, F. Eisenhaber, W. J. Rettig, and M. C. Lenter Molecular Cloning and Characterization of EndoGlyx-1, an EMILIN-like Multisubunit Glycoprotein of Vascular Endothelium J. Biol. Chem., December 14, 2001; 276(51): 48588 - 48595. [Abstract] [Full Text] [PDF]

				W. H. A. Kahr, S. Zheng, P. M. Sheth, M. Pai, A. Cowie, M. Bouchard, T. J. Podor, G. E. Rivard, and C. P. M. Hayward Platelets from patients with the Quebec platelet disorder contain and secrete abnormal amounts of urokinase-type plasminogen activator Blood, July 15, 2001; 98(2): 257 - 265. [Abstract] [Full Text] [PDF]

				U. Kishore, P. Strong, M. V. Perdikoulis, and K. B. M. Reid A Recombinant Homotrimer, Composed of the {{alpha}} Helical Neck Region of Human Surfactant Protein D and C1q B Chain Globular Domain, Is an Inhibitor of the Classical Complement Pathway J. Immunol., January 1, 2001; 166(1): 559 - 565. [Abstract] [Full Text] [PDF]

				R. Doliana, A. Canton, F. Bucciotti, M. Mongiat, P. Bonaldo, and A. Colombatti Structure, Chromosomal Localization, and Promoter Analysis of the Human Elastin Microfibril Interfase Located proteIN (EMILIN) Gene J. Biol. Chem., January 14, 2000; 275(2): 785 - 792. [Abstract] [Full Text] [PDF]

				C. P.M. Hayward, Z. Song, S. Zheng, R. Fung, M. Pai, J.-M. Masse, and E. M. Cramer Multimerin Processing by Cells With and Without Pathways for Regulated Protein Secretion Blood, August 15, 1999; 94(4): 1337 - 1347. [Abstract] [Full Text] [PDF]

				Y. Zhang and Q. Chen The Noncollagenous Domain 1 of Type X Collagen. A NOVEL MOTIF FOR TRIMER AND HIGHER ORDER MULTIMER FORMATION WITHOUT A TRIPLE HELIX J. Biol. Chem., August 6, 1999; 274(32): 22409 - 22413. [Abstract] [Full Text] [PDF]

				B. Dublet, T. Vernet, and M. van der Rest Schmid's Metaphyseal Chondrodysplasia Mutations Interfere with Folding of the C-terminal Domain of Human Collagen X Expressed in Escherichia coli J. Biol. Chem., July 2, 1999; 274(27): 18909 - 18915. [Abstract] [Full Text] [PDF]

				R. Doliana, M. Mongiat, F. Bucciotti, E. Giacomello, R. Deutzmann, D. Volpin, G. M. Bressan, and A. Colombatti EMILIN, a Component of the Elastic Fiber and a New Member of the C1q/Tumor Necrosis Factor Superfamily of Proteins J. Biol. Chem., June 11, 1999; 274(24): 16773 - 16781. [Abstract] [Full Text] [PDF]

				C. P. M. Hayward, E. M. Cramer, Z. Song, S. Zheng, R. Fung, J.-M. Masse, R. H. Stead, and T. J. Podor Studies of Multimerin in Human Endothelial Cells Blood, February 15, 1998; 91(4): 1304 - 1317. [Abstract] [Full Text] [PDF]

				C. P.M. Hayward, E. M. Cramer, W. H. Kane, S. Zheng, M. Bouchard, J.-M. Masse, and G. E. Rivard Studies of a Second Family With the Quebec Platelet Disorder: Evidence That the Degradation of the alpha -Granule Membrane and Its Soluble Contents Are Not Secondary to a Defect in Targeting Proteins to alpha -Granules Blood, February 15, 1997; 89(4): 1243 - 1253. [Abstract] [Full Text] [PDF]

				M. Mongiat, G. Mungiguerra, S. Bot, M. T. Mucignat, E. Giacomello, R. Doliana, and A. Colombatti Self-assembly and Supramolecular Organization of EMILIN J. Biol. Chem., August 11, 2000; 275(33): 25471 - 25480. [Abstract] [Full Text] [PDF]