| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, June 1,
1995; and in revised form, June 27, 1995) Tyrosine phosphorylation of cellular proteins is an early and an
essential step in T cell receptor-mediated lymphocyte activation.
Tyrosine phosphorylation of transmembrane receptor chains (such as
Volume 270,
Number 34,
Issue of August 25, pp. 20177-20182, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
1-binding
Phosphotyrosyl Protein pp36/38
and CD3 chains) and membrane-associated proteins provides docking
sites for SH2 domains of adaptor proteins and signaling enzymes,
resulting in their recruitment in the vicinity of activated receptors.
pp36/38 is a prominent substrate of early tyrosine phosphorylation upon
stimulation through the T cell receptor. The tyrosine-phosphorylated
form of pp36/38 is membrane-associated and directly interacts with
phospholipase C-
1 and Grb2, providing one mechanism to recruit
downstream effectors to the cell membrane. Here, we demonstrate that in
Jurkat T cells, pp36/38 associates with the p85 subunit of
phosphatidylinositol 3-kinase (PI-3-K p85) in an activation-dependent
manner. Association of pp36/38 with PI-3-K p85 was confirmed by
transfection of a hemagglutinin-tagged p85
cDNA into Jurkat cells
followed by anti-hemagglutinin immunoprecipitation. In vitro binding experiments with glutathione S-transferase fusion
proteins of PI-3-K p85 demonstrated that the SH2 domains, but not the
SH3 domain, mediated binding to pp36/38. This binding was selectively
abrogated by phosphopeptides that bind to p85 SH2 domains with high
affinity. Filter binding assays demonstrated that association between
pp36/38 and PI-3-K p85 SH2 domains was due to direct binding. These
results strongly suggest the role of pp36/38 in recruiting PI-3-K to
the cell membrane and further support the idea that pp36/38 is a
multifunctional docking protein for SH2 domain-containing signaling
proteins in T cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. H. Wabnitz, G. Nebl, M. Klemke, A. J. Schroder, and Y. Samstag Phosphatidylinositol 3-Kinase Functions as a Ras Effector in the Signaling Cascade That Regulates Dephosphorylation of the Actin-Remodeling Protein Cofilin after Costimulation of Untransformed Human T Lymphocytes J. Immunol., February 1, 2006; 176(3): 1668 - 1674. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-S. Li, W. Ma, H. Jaffe, Y. Zheng, S. Takahashi, L. Zhang, A. B. Kulkarni, and H. C. Pant Cyclin-dependent Kinase-5 Is Involved in Neuregulin-dependent Activation of Phosphatidylinositol 3-Kinase and Akt Activity Mediating Neuronal Survival J. Biol. Chem., September 12, 2003; 278(37): 35702 - 35709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Aandahl, W. J. Moretto, P. A. Haslett, T. Vang, T. Bryn, K. Tasken, and D. F. Nixon Inhibition of Antigen-Specific T Cell Proliferation and Cytokine Production by Protein Kinase A Type I J. Immunol., July 15, 2002; 169(2): 802 - 808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. W. H. G. Kessels, A. C. Ward, and T. N. M. Schumacher Specificity and affinity motifs for Grb2 SH2-ligand interactions PNAS, June 25, 2002; 99(13): 8524 - 8529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. I. Gringhuis, E. A. M. Papendrecht-van der Voort, A. Leow, E. W. N. Levarht, F. C. Breedveld, and C. L. Verweij Effect of Redox Balance Alterations on Cellular Localization of LAT and Downstream T-Cell Receptor Signaling Pathways Mol. Cell. Biol., January 15, 2002; 22(2): 400 - 411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Wilson, J. R. Pfeiffer, Z. Surviladze, E. A. Gaudet, and J. M. Oliver High resolution mapping of mast cell membranes reveals primary and secondary domains of Fc{epsilon}RI and LAT J. Cell Biol., August 6, 2001; 154(3): 645 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. I. Gringhuis, A. Leow, E. A. M. Papendrecht-van der Voort, P. H. J. Remans, F. C. Breedveld, and C. L. Verweij Displacement of Linker for Activation of T Cells from the Plasma Membrane Due to Redox Balance Alterations Results in Hyporesponsiveness of Synovial Fluid T Lymphocytes in Rheumatoid Arthritis J. Immunol., February 15, 2000; 164(4): 2170 - 2179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Lindholm, E. Gylfe, W. Zhang, L. E. Samelson, and M. Welsh Requirement of the Src Homology 2 Domain Protein Shb for T Cell Receptor-dependent Activation of the Interleukin-2 Gene Nuclear Factor for Activation of T Cells Element in Jurkat T Cells J. Biol. Chem., September 24, 1999; 274(39): 28050 - 28057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q.-s. Ji, A. Chattopadhyay, M. Vecchi, and G. Carpenter Physiological Requirement for Both SH2 Domains for Phospholipase C-gamma 1 Function and Interaction with Platelet-Derived Growth Factor Receptors Mol. Cell. Biol., July 1, 1999; 19(7): 4961 - 4970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-L. Law, M. K. Ewings, P. M. Chaudhary, S. A. Solow, T. J. Yun, A. J. Marshall, L. Hood, and E. A. Clark GrpL, a Grb2-related Adaptor Protein, Interacts with SLP-76 to Regulate Nuclear Factor of Activated T Cell Activation J. Exp. Med., April 19, 1999; 189(8): 1243 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Facchetti, J. K. C. Chan, W. Zhang, A. Tironi, M. Chilosi, S. Parolini, L. D. Notarangelo, and L. E. Samelson Linker for Activation of T Cells (LAT), a Novel Immunohistochemical Marker for T Cells, NK Cells, Mast Cells, and Megakaryocytes : Evaluation in Normal and Pathological Conditions Am. J. Pathol., April 1, 1999; 154(4): 1037 - 1046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Gibbins, S. Briddon, A. Shutes, M. J. van Vugt, J. G. J. van de Winkel, T. Saito, and S. P. Watson The p85 Subunit of Phosphatidylinositol 3-Kinase Associates with the Fc Receptor gamma -Chain and Linker for Activitor of T Cells (LAT) in Platelets Stimulated by Collagen and Convulxin J. Biol. Chem., December 18, 1998; 273(51): 34437 - 34443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. Kyono, R. de Jong, R. Kil Park, Y. Liu, N. Heisterkamp, J. Groffen, and D. L. Durden Differential Interaction of Crkl with Cbl or C3G, Hef-1, and {gamma} Subunit Immunoreceptor Tyrosine-Based Activation Motif in Signaling of Myeloid High Affinity Fc Receptor for IgG (Fc{gamma}RI) J. Immunol., November 15, 1998; 161(10): 5555 - 5563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Stoica, K. E. DeBell, L. Graham, B. L. Rellahan, M. A. Alava, J. Laborda, and E. Bonvini The Amino-Terminal Src Homology 2 Domain of Phospholipase C{gamma}1 Is Essential for TCR-Induced Tyrosine Phosphorylation of Phospholipase C{gamma}1 J. Immunol., February 1, 1998; 160(3): 1059 - 1066. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Salojin, J. Zhang, M. Cameron, B. Gill, G. Arreaza, A. Ochi, and T. L. Delovitch Impaired Plasma Membrane Targeting of Grb2-Murine Son of Sevenless (mSOS) Complex and Differential Activation of the Fyn-T Cell Receptor (TCR)-zeta -Cbl Pathway Mediate T Cell Hyporesponsiveness in Autoimmune Nonobese Diabetic Mice J. Exp. Med., September 15, 1997; 186(6): 887 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takaki, J. D. Watts, K. A. Forbush, N. T. Nguyen, J. Hayashi, J. Alberola-Ila, R. Aebersold, and R. M. Perlmutter Characterization of Lnk. AN ADAPTOR PROTEIN EXPRESSED IN LYMPHOCYTES J. Biol. Chem., June 6, 1997; 272(23): 14562 - 14570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Luo and J. J. Sando Deficient Tyrosine Phosphorylation of c-Cbl and Associated Proteins in Phorbol Ester-resistant EL4 Mouse Thymoma Cells J. Biol. Chem., May 2, 1997; 272(18): 12221 - 12228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Ganju, W. C. Hatch, H. Avraham, M. A. Ona, B. Druker, S. Avraham, and J. E. Groopman RAFTK, a Novel Member of the Focal Adhesion Kinase Family, Is Phosphorylated and Associates with Signaling Molecules upon Activation of Mature T Lymphocytes J. Exp. Med., March 17, 1997; 185(6): 1055 - 1064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Trub, J. D. Frantz, M. Miyazaki, H. Band, and S. E. Shoelson The Role of a Lymphoid-restricted, Grb2-like SH3-SH2-SH3 Protein in T Cell Receptor Signaling J. Biol. Chem., January 10, 1997; 272(2): 894 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hartley and S. Corvera Formation of c-Cbl·Phosphatidylinositol 3-Kinase Complexes on Lymphocyte Membranes by a p56lck-independent Mechanism J. Biol. Chem., September 6, 1996; 271(36): 21939 - 21943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fukazawa, S. Miyake, V. Band, and H. Band Tyrosine Phosphorylation of Cbl upon Epidermal Growth Factor (EGF) Stimulation and Its Association with EGF Receptor and Downstream Signaling Proteins J. Biol. Chem., June 14, 1996; 271(24): 14554 - 14559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Goh, Y. P. Lim, S. H. Ong, C. B. Siak, X. Cao, Y. H. Tan, and G. R. Guy Identification of p90, a Prominent Tyrosine-phosphorylated Protein in Fibroblast Growth Factor-stimulated Cells, as 80K-H J. Biol. Chem., March 8, 1996; 271(10): 5832 - 5838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Panchamoorthy, T. Fukazawa, S. Miyake, S. Soltoff, K. Reedquist, B. Druker, S. Shoelson, L. Cantley, and H. Band p120[IMAGE] Is a Major Substrate of Tyrosine Phosphorylation upon B Cell Antigen Receptor Stimulation and Interacts in Vivo with Fyn and Syk Tyrosine Kinases, Grb2 and Shc Adaptors, and the p85 Subunit of Phosphatidylinositol 3-Kinase J. Biol. Chem., February 9, 1996; 271(6): 3187 - 3194. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
