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Volume 270,
Number 38,
Issue of September 22, pp. 22406-22411, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Identification of
Kv1.1 Expression by Murine CD4 CD8 Thymocytes
A ROLE FOR VOLTAGE-DEPENDENT K CHANNELS IN MURINE
THYMOCYTE DEVELOPMENT
(Received for publication, April 17, 1995; and in revised form, June 20, 1995)
Bruce D.
Freedman
, <WBR>
Bernd K.
Fleischmann
, <WBR>
Jennifer A.
Punt
, <WBR>
Glen
Gaulton
, <WBR>
Yasuhiro
Hashimoto
, <WBR>
Michael I.
Kotlikoff
The patch-clamp recording technique and RNApolymerase chain
reaction were used to identify the voltage-dependent K channels expressed by murine fetal and adult
CD4 CD8 thymocytes. Two distinct
currents, encoded by the genes Kv1.1 and Kv1.3 were identified based
upon their biophysical and pharmacologic characteristics and confirmed
with RNA-polymerase chain reaction. Peptide blockers of Kv1.1 and Kv1.3
gene products were also applied to a murine fetal thymic organ culture
system to investigate the developmental role of these K channels. Dendrotoxin (DTX) and charybdotoxin (CTX), antagonists
of Kv1.1 and Kv1.3 channels, respectively, decreased thymocyte yields
in organ culture without affecting thymocyte viability. DTX-treated
thymi contained 56 ± 8% (n = 8 experiments), and
CTX-treated thymi contained 74 ± 4% (n = 7
experiments) as many thymocytes as untreated lobes. DTX and CTX also
altered the developmental progression of thymocytes in fetal organ
culture. These data provide the first evidence of Kv1.1 expression in a
lymphoid cell and indicate that thymocyte voltage-dependent
K channels are critical to thymocyte preclonal
expansion and/or maturation.

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Copyright © 1995 by the American Society for Biochemistry and Molecular Biology.
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