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Volume 270, Number 39, Issue of September 29, pp. 22665-22668, 1995
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
The Interaction of TFIIIA with Specific RNA-DNA Heteroduplexes

(Received for publication, July 10, 1995; and in revised form, August 1, 1995)

Karl P. Nightingale,  Alan P. Wolffe

We examine the association of transcription factor TFIIIA with RNA-DNA heteroduplexes containing sequences from the Xenopus borealis 5 S rRNA gene. Under conditions where TFIIIA selectively binds to 5 S rRNA or the internal control region of the 5 S rRNA gene, no specific association of TFIIIA with DNA-RNA heteroduplexes containing either strand of 5 S DNA could be detected. We discuss our results with respect to specific models of TFIIIA recognition of the internal control region and of DNA-RNA hybrids by zinc finger proteins.



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B. S. Shastry
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J. Cell Sci., March 1, 1996; 109(3): 535 - 539.
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