CD16, the low affinity Fc receptor III for IgG (FcRIII), exists as a polypeptide-anchored form (FcRIIIA or CD16A) in human natural killer cells and macrophages and as a glycosylphosphatidylinositol-anchored form (FcRIIIB or CD16B) in neutrophils. CD16A requires association of the subunit of FcRI or the subunit of the TCR-CD3 complex for cell surface expression. The CD16B is polymorphic and the two alleles are termed NA1 and NA2. In this study, CD16A and the two alleles of CD16B have been expressed in Chinese hamster ovary (CHO) cells and their ligand binding and phagocytic properties analyzed. The two allelic forms of CD16B showed a similar affinity toward human IgG1. However, the NA1 allele showed approximately 2-fold higher affinity for the IgG3 than the NA2 allele. Although all three forms of CD16 efficiently bound rabbit IgG-coated erythrocytes (EA), only CD16A coexpressed with the subunit phagocytosed EA. The phagocytosis mediated by CD16A expressed on CHO cells was independent of divalent cations but dependent on intact microfilaments. CHO cells expressing CD16A- and CD16A- chimeras also phagocytosed EA. The phagocytosis was specifically inhibited by tyrphostin-23, a tyrosine kinase inhibitor. In summary, our results demonstrate that glycosylphosphatidylinositol-anchored CD16B alleles differ from CD16A in their ability to mediate phagocytosis. Furthermore, since studies with other FcRs have shown that CHO cells lack the phagocytic pathway mediated by the cytoplasmic domain of FcRs, the phagocytosis of EA by CHO cells stably transfected with CD16A and CD16A-subunit chimera provides an ideal system to dissect the phagocytic signaling pathways mediated by these FcR-associated subunits.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				P. Bruhns, B. Iannascoli, P. England, D. A. Mancardi, N. Fernandez, S. Jorieux, and M. Daeron Specificity and affinity of human Fc{gamma} receptors and their polymorphic variants for human IgG subclasses Blood, April 16, 2009; 113(16): 3716 - 3725. [Abstract] [Full Text] [PDF]

				N. Meknache, F. Jonsson, J. Laurent, M.-T. Guinnepain, and M. Daeron Human Basophils Express the Glycosylphosphatidylinositol-Anchored Low-Affinity IgG Receptor Fc{gamma}RIIIB (CD16B) J. Immunol., February 15, 2009; 182(4): 2542 - 2550. [Abstract] [Full Text] [PDF]

				L. C. Willcocks, P. A. Lyons, M. R. Clatworthy, J. I. Robinson, W. Yang, S. A. Newland, V. Plagnol, N. N. McGovern, A. M. Condliffe, E. R. Chilvers, et al. Copy number of FCGR3B, which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake J. Exp. Med., July 7, 2008; 205(7): 1573 - 1582. [Abstract] [Full Text] [PDF]

				R. Shashidharamurthy, R. A. Hennigar, S. Fuchs, P. Palaniswami, M. Sherman, and P. Selvaraj Extravasations and emigration of neutrophils to the inflammatory site depend on the interaction of immune-complex with Fc{gamma} receptors and can be effectively blocked by decoy Fc{gamma} receptors Blood, January 15, 2008; 111(2): 894 - 904. [Abstract] [Full Text] [PDF]

				P. Li, N. Jiang, S. Nagarajan, R. Wohlhueter, P. Selvaraj, and C. Zhu Affinity and Kinetic Analysis of Fc{gamma} Receptor IIIa (CD16a) Binding to IgG Ligands J. Biol. Chem., March 2, 2007; 282(9): 6210 - 6221. [Abstract] [Full Text] [PDF]

				S. Nagarajan, N. H. Fifadara, and P. Selvaraj Signal-Specific Activation and Regulation of Human Neutrophil Fc{gamma} Receptors J. Immunol., May 1, 2005; 174(9): 5423 - 5432. [Abstract] [Full Text] [PDF]

				L. Peiser, P. J. Gough, T. Kodama, and S. Gordon Macrophage Class A Scavenger Receptor-Mediated Phagocytosis of Escherichia coli: Role of Cell Heterogeneity, Microbial Strain, and Culture Conditions In Vitro Infect. Immun., April 1, 2000; 68(4): 1953 - 1963. [Abstract] [Full Text] [PDF]

				S. E. Chesla, P. Li, S. Nagarajan, P. Selvaraj, and C. Zhu The Membrane Anchor Influences Ligand Binding Two-dimensional Kinetic Rates and Three-dimensional Affinity of Fcgamma RIII (CD16) J. Biol. Chem., March 31, 2000; 275(14): 10235 - 10246. [Abstract] [Full Text] [PDF]

				S. Nagarajan, K. Venkiteswaran, M. Anderson, U. Sayed, C. Zhu, and P. Selvaraj Cell-specific, activation-dependent regulation of neutrophil CD32A ligand-binding function Blood, February 1, 2000; 95(3): 1069 - 1077. [Abstract] [Full Text] [PDF]

				R. J. Botelho, D. J. Hackam, A. D. Schreiber, and S. Grinstein Role of COPI in Phagosome Maturation J. Biol. Chem., May 19, 2000; 275(21): 15717 - 15727. [Abstract] [Full Text] [PDF]