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Volume 270,
Number 43,
Issue of October 27, 1995 pp. 25968-25975
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Transcription
of the blk Gene in Human B Lymphocytes Is Controlled by Two
Promoters
(Received for publication, July 6, 1995)
Yu-Huei
Lin,
Edward
J.
Shin,
Michael J.
Campbell,
John
E.
Niederhuber
Genomic DNA containing the first exon and 5`-flanking region of
the human protein tyrosine kinase, blk, was isolated. Sequence
analysis identified a TG repeat element in this region with enhancer
activity, but no TATA or CCAAT sequences were found. Two blk transcripts of 2.2 and 2.5 kilobases were identified in various
B-cell lines by Northern blot analyses, and primer extension
experiments demonstrated two clusters of multiple transcription start
sites. Subsequent promoter analyses by transient transfection assays
with a reporter gene identified two promoter elements in the human blk gene. Promoter P1 contains sequences that have been shown
to regulate the expression of immunoglobulin genes and promoter P2
contains elements that are highly conserved in the promoter of major
histocompatibility complex class II genes, as well as a B-cell-specific
activator protein- (BSAP) binding site. Electrophoretic mobility shift
assays demonstrated that the binding of a protein to the BSAP-binding
site was correlated with the presence of the 2.5-kilobase blk transcript. These data suggest that the two human blk RNAs arise from the transcription of the blk gene by two
distinct promoters and that these promoters may be subject to
regulation by different trans-acting factors.

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Copyright © 1995 by the American Society for Biochemistry and Molecular Biology.
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