Recently we reported the localization of phosphoinositide 3-kinase (PI 3-kinase) by immunofluorescence to microtubule bundles and the centrosome (Kapeller, R., Chakrabarti, R., Cantley, L., Fay, F., and Corvera, S.(1993) Mol. Cell. Biol. 13, 6052-6063). In complementary experiments we used the recombinant p85 subunit of PI 3-kinase to identify proteins that associate with phosphoinositide 3-kinase and found that phosphoinositide 3-kinase associates with /-tubulin. The association occurs in vivo but was not significantly affected by growth factor stimulation. We localized the region of p85 that interacts with /-tubulin to the inter-SH2 domain. These results support the immunofluorescence data and show that p85 directly associates with /-tubulin. We then determined whether phosphoinositide 3-kinase associates with -tubulin. We found a dramatic growth factor-dependent association of phosphoinositide 3-kinase with -tubulin. Phosphoinositide 3-kinase associates with -tubulin in response to insulin and, to a lesser extent, in response to platelet-derived growth factor. Neither epidermal growth factor nor nerve growth factor treatment of cells results in association of phosphoinositide 3-kinase and -tubulin. Phosphoinositide 3-kinase is also immunoprecipitated with antibodies to pericentrin in response to insulin, indicating that phosphoinositide 3-kinase is recruited to the centrosome. Neither phosphoinositide 3-kinase activity, nor intact microtubules are necessary for the association. Treatment of cells with 0.5 M NaCl dissociates -tubulin from the centrosome and disrupts the association of phosphoinositide 3-kinase with pericentrin, but not -tubulin. Recombinant p85 binds to -tubulin from both insulin stimulated and quiescent cells. These results suggest that the association of phosphoinositide 3-kinase with -tubulin is direct. These data suggest that phosphoinositide 3-kinase may be involved in regulating microtubule responses to insulin and platelet-derived growth factor.

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