Reduced peptide bond pseudopeptide analogues have been examined for their ability to bind murine class I molecules of the major histocompatibility complex (MHC). Eight pseudopeptide analogues of an antigenic peptide derived from Plasmodium berghei (H-Ser-Tyr-Ile-Pro-Ser-Ala-Glu-Lys-Ile-OH) were obtained by systematically replacing one peptide bond at a time by a reduced peptide bond (CH-NH). The resulting analogues were then tested for their binding to a recombinant single chain SC-K class I molecule. The comparative results show that five analogues can efficiently mimic the parent peptide while the introduction of the reduced bond between P3-P4, P7-P8, and P8-P9 is deleterious for SC-K binding. The fact that more stable pseudopeptides containing reduced peptide bonds can bind major histocompatibility complex molecules is of great interest for the design of peptidomimetics with potential therapeutical properties. Such peptide analogues may prove useful for the development of peptide-based cytotoxic T lymphocyte vaccines.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. I. Webb, M. A. Dunstone, W. Chen, M.-I. Aguilar, Q. Chen, H. Jackson, L. Chang, L. Kjer-Nielsen, T. Beddoe, J. McCluskey, et al. Functional and Structural Characteristics of NY-ESO-1-related HLA A2-restricted Epitopes and the Design of a Novel Immunogenic Analogue J. Biol. Chem., May 28, 2004; 279(22): 23438 - 23446. [Abstract] [Full Text] [PDF]

				S. Calbo, G. Guichard, P. Bousso, S. Muller, P. Kourilsky, J.-P. Briand, and J.-P. Abastado Role of Peptide Backbone in T Cell Recognition J. Immunol., April 15, 1999; 162(8): 4657 - 4662. [Abstract] [Full Text] [PDF]

				M. Ayyoub, H. Mazarguil, B. Monsarrat, B. Van den Eynde, and J. E. Gairin A Structure-based Approach to Designing Non-natural Peptides That Can Activate Anti-melanoma Cytotoxic T Cells J. Biol. Chem., April 9, 1999; 274(15): 10227 - 10234. [Abstract] [Full Text] [PDF]

				C. Stemmer, A. Quesnel, A. Prevost-Blondel, C. Zimmermann, S. Muller, J.-P. Briand, and H. Pircher Protection against Lymphocytic Choriomeningitis Virus Infection Induced by a Reduced Peptide Bond Analogue of the H-2Db-restricted CD8+ T Cell Epitope GP33 J. Biol. Chem., February 26, 1999; 274(9): 5550 - 5556. [Abstract] [Full Text] [PDF]

				A. Bianco, C. Brock, C. Zabel, T. Walk, P. Walden, and G. Jung New Synthetic Non-peptide Ligands for Classical Major Histocompatibility Complex Class I Molecules J. Biol. Chem., October 30, 1998; 273(44): 28759 - 28765. [Abstract] [Full Text] [PDF]

				N. Benkirane, G. Guichard, J.-P. Briand, and S. Muller Exploration of Requirements for Peptidomimetic Immune Recognition. ANTIGENIC AND IMMUNOGENIC PROPERTIES OF REDUCED PEPTIDE BOND PSEUDOPEPTIDE ANALOGUES OF A HISTONE HEXAPEPTIDE J. Biol. Chem., December 27, 1996; 271(52): 33218 - 33224. [Abstract] [Full Text] [PDF]