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Volume 270,
Number 45,
Issue of November 10, 1995 pp. 26786-26789
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Cloning
of a cDNA for the Type II Iodothyronine Deiodinase
(Received for publication, August 3,
1995; and in revised form, August 23, 1995)
Jennifer C.
Davey,
Kathryn B.
Becker ,
Mark J.
Schneider,
Donald L. St.
Germain ,
Valerie Anne
Galton
Three types of iodothyronine deiodinase have been identified in
vertebrate tissues. cDNAs for the types I and III have been cloned and
shown to contain an in-frame TGA that codes for selenocysteine at the
active site of the enzyme. We now report the cloning of a cDNA for a
type II deiodinase using a reverse transcription/polymerase chain
reaction strategy and RNA obtained from Rana catesbeiana tissues. This cDNA (RC5`DII) manifests limited but significant
homology with other deiodinase cDNAs and contains a conserved in-frame
TGA codon. Injection of capped in vitro synthesized
transcripts of the cDNA into Xenopuslaevis oocytes
results in the induction of deiodinase activity with characteristics
typical of a type II deiodinase. The levels of RC5`DII transcripts in R. catesbeiana tadpole tail and liver mRNA at stages XII and
XXIII correspond well with that of type II deiodinase activity but not
that of the type III activity in these tissues. These findings indicate
that the amphibian type II 5`-deiodinase is a structurally unique
member of the family of selenocysteine-containing deiodinases.

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|
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|
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|
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|
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|
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|
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|
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|
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|
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|
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H. Huang, L. Cai, B. F. Remo, and D. D. Brown
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Copyright © 1995 by the American Society for Biochemistry and Molecular Biology.
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