Monoclonal antibodies were raised to membrane-bound proteoglycans derived from rat brain, and four monoclonal antibodies that recognized a 150-kDa chondroitin sulfate proteoglycan with a core glycoprotein of 120 kDa were obtained. Immunohistological study revealed that the proteoglycan was associated with developing neurons. We screened rat brain cDNA libraries using the four monoclonal antibodies and isolated overlapping cDNA clones that encoded the entire core protein of 514 amino acids plus a 30-residue signal peptide. The deduced amino acid sequence suggested an integral membrane protein divided into five structurally different domains: an N-terminal domain to which chondroitin sulfate chains might be attached, a basic amino acid cluster consisting of seven arginine and two lysine residues, a cysteine-containing domain, a membrane-spanning segment, and a C-terminal cytoplasmic domain of 95 amino acids. On Northern blots, the cDNA hybridized with a single mRNA of 3.1 kilobases that was detectable in brains of neonatal and adult rats but not in kidney, liver, lung, and muscle of either. The sequence of the proteoglycan did not exhibit significant homology to any other known protein, indicating that the proteoglycan, designated neuroglycan C, is a novel integral membrane proteoglycan.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. Purushothaman, J. Fukuda, S. Mizumoto, G. B. ten Dam, T. H. van Kuppevelt, H. Kitagawa, T. Mikami, and K. Sugahara Functions of Chondroitin Sulfate/Dermatan Sulfate Chains in Brain Development: CRITICAL ROLES OF E AND iE DISACCHARIDE UNITS RECOGNIZED BY A SINGLE CHAIN ANTIBODY GD3G7 J. Biol. Chem., July 6, 2007; 282(27): 19442 - 19452. [Abstract] [Full Text] [PDF]

				K. Ichihara-Tanaka, A. Oohira, M. Rumsby, and T. Muramatsu Neuroglycan C Is a Novel Midkine Receptor Involved in Process Elongation of Oligodendroglial Precursor-like Cells J. Biol. Chem., October 13, 2006; 281(41): 30857 - 30864. [Abstract] [Full Text] [PDF]

				K. Nakanishi, S. Aono, K. Hirano, Y. Kuroda, M. Ida, Y. Tokita, F. Matsui, and A. Oohira Identification of Neurite Outgrowth-promoting Domains of Neuroglycan C, a Brain-specific Chondroitin Sulfate Proteoglycan, and Involvement of Phosphatidylinositol 3-Kinase and Protein Kinase C Signaling Pathways in Neuritogenesis J. Biol. Chem., August 25, 2006; 281(34): 24970 - 24978. [Abstract] [Full Text] [PDF]

				S. S. Deepa, D. Carulli, C. Galtrey, K. Rhodes, J. Fukuda, T. Mikami, K. Sugahara, and J. W. Fawcett Composition of Perineuronal Net Extracellular Matrix in Rat Brain: A DIFFERENT DISACCHARIDE COMPOSITION FOR THE NET-ASSOCIATED PROTEOGLYCANS J. Biol. Chem., June 30, 2006; 281(26): 17789 - 17800. [Abstract] [Full Text] [PDF]

				S. Aono, Y. Tokita, T. Shuo, S. Yamauchi, F. Matsui, K. Nakanishi, K. Hirano, M. Sano, and A. Oohira Glycosylation Site for Chondroitin Sulfate on the Neural Part-time Proteoglycan, Neuroglycan C J. Biol. Chem., November 5, 2004; 279(45): 46536 - 46541. [Abstract] [Full Text] [PDF]

				B. Hassel, M. Schreff, E.-M. Stube, U. Blaich, and S. Schumacher CALEB/NGC Interacts with the Golgi-associated Protein PIST J. Biol. Chem., October 10, 2003; 278(41): 40136 - 40143. [Abstract] [Full Text] [PDF]

				S. Yamauchi, Y. Tokita, S. Aono, F. Matsui, T. Shuo, H. Ito, K. Kato, K. Kasahara, and A. Oohira Phosphorylation of Neuroglycan C, a Brain-specific Transmembrane Chondroitin Sulfate Proteoglycan, and Its Localization in the Lipid Rafts J. Biol. Chem., May 31, 2002; 277(23): 20583 - 20590. [Abstract] [Full Text] [PDF]

				M. Inatani, H. Tanihara, A. Oohira, Y. Otori, A. Nishida, M. Honjo, N. Kido, and Y. Honda Neuroglycan C, a Neural Tissue-Specific Transmembrane Chondroitin Sulfate Proteoglycan, in Retinal Neural Network Formation Invest. Ophthalmol. Vis. Sci., December 1, 2000; 41(13): 4338 - 4346. [Abstract] [Full Text]

				C. E. Bandtlow and D. R. Zimmermann Proteoglycans in the Developing Brain: New Conceptual Insights for Old Proteins Physiol Rev, October 1, 2000; 80(4): 1267 - 1290. [Abstract] [Full Text] [PDF]

				S. Yamauchi, S. Mita, T. Matsubara, M. Fukuta, H. Habuchi, K. Kimata, and O. Habuchi Molecular Cloning and Expression of Chondroitin 4-Sulfotransferase J. Biol. Chem., March 17, 2000; 275(12): 8975 - 8981. [Abstract] [Full Text] [PDF]

				S. Acharya, V. C. Foletta, J. W. Lee, M. E. Rayborn, I. R. Rodriguez, W. S. Young III, and J. G. Hollyfield SPACRCAN, a Novel Human Interphotoreceptor Matrix Hyaluronan-binding Proteoglycan Synthesized by Photoreceptors and Pinealocytes J. Biol. Chem., March 15, 2000; 275(10): 6945 - 6955. [Abstract] [Full Text] [PDF]

				S. Aono, H. Keino, T. Ono, Y. Yasuda, Y. Tokita, F. Matsui, M. Taniguchi, S.-i. Sonta, and A. Oohira Genomic Organization and Expression Pattern of Mouse Neuroglycan C in the Cerebellar Development J. Biol. Chem., January 7, 2000; 275(1): 337 - 342. [Abstract] [Full Text] [PDF]

				S. Schumacher, H. Volkmer, F. Buck, A. Otto, A. Tarnok, S. Roth, and F. G. Rathjen Chicken Acidic Leucine-rich EGF-like Domain Containing Brain Protein (CALEB), a Neural Member of the EGF Family of Differentiation Factors, Is Implicated in Neurite Formation J. Cell Biol., February 24, 1997; 136(4): 895 - 906. [Abstract] [Full Text] [PDF]

				C. Ueoka, N. Kaneda, I. Okazaki, S. Nadanaka, T. Muramatsu, and K. Sugahara Neuronal Cell Adhesion, Mediated by the Heparin-binding Neuroregulatory Factor Midkine, Is Specifically Inhibited by Chondroitin Sulfate E. STRUCTURAL AND FUNCTIONAL IMPLICATIONS OF THE OVER-SULFATED CHONDROITIN SULFATE J. Biol. Chem., November 22, 2000; 275(48): 37407 - 37413. [Abstract] [Full Text] [PDF]

				S. Schumacher, M. Jung, U. Norenberg, A. Dorner, R. Chiquet-Ehrismann, C. A. O. Stuermer, and F. G. Rathjen CALEB Binds via Its Acidic Stretch to the Fibrinogen-like Domain of Tenascin-C or Tenascin-R and Its Expression Is Dynamically Regulated after Optic Nerve Lesion J. Biol. Chem., March 2, 2001; 276(10): 7337 - 7345. [Abstract] [Full Text] [PDF]