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(Received for publication, August 28,
1995; and in revised form, September 22, 1995) We find that reconstituted nucleosome cores containing specific
DNA sequences dissociate on dilution. This disruption of histone-DNA
contacts leading to the release of free DNA is facilitated by the
presence of the core histone tails, MgCl
Volume 270,
Number 46,
Issue of November 17, 1995 pp. 27399-27402
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
THE EFFECT OF PARTICLE CONCENTRATION, MgCl
AND KCl
CONCENTRATION, THE HISTONE TAILS, AND TEMPERATURE
(5 mM),
KCl (60 mM), and temperatures above 0 °C. Under reaction
conditions that are commonly used to assess trans-acting factor access
to nucleosomal DNA, histone-DNA contacts are on the threshold of
instability. We demonstrate how dilution of reconstituted nucleosomes
containing a TATA box can facilitate TBP access to DNA.
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