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(Received for publication, August 14, 1995)
Cyclin B1 mRNA expression varies through the cell cycle with its
peak in G
/M. In cycling mammalian cells, its lowest level
is in G
with a steady increase in S until a level 50-fold
greater than that in G is reached. In order to characterize
the transcriptional component to this variation in expression, we
cloned the upstream region 872 base pairs upstream from the start site
of the cyclin B1 gene and have demonstrated that it confers cell
cycle-dependent regulation onto two reporter genes, both
chloramphenicol acetyltransferase and luciferase. Its activity was
25-fold greater in G/M than in G in HeLa cells
with intermediate activity in S. This cyclical activity could be seen
with sequences encompassing only 90 base pairs upstream from the start
site. Protein binding to this region was demonstrated using
electrophoretic mobility shift assays, and the binding profiles
appeared to vary depending upon the phase of the cycle in which the
extracts are made. Thus, transcriptional control plays an important
role in determining cyclin B1 mRNA levels, and cell cycle-dependent
activity is regulated through interactions with the region 90 bases
upstream from the start site.
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