The carbon storage regulator gene csrA has been shown previously to dramatically affect the biosynthesis of intracellular glycogen in Escherichia coli through its negative control of the expression of two glycogen biosynthetic operons and the gluconeogenic gene pckA (Romeo, T., Gong, M., Liu, M. Y., and Brun-Zinkernagel, A. M.(1993) J. Bacteriol. 175, 4744-4755). Examination of the effects of csrA on several enzymes, genes, and metabolites of central carbohydrate metabolism now establishes a more extensive role for csrA in directing intracellular carbon flux. Phosphoglucomutase and the gluconeogenic enzymes fructose-1,6-bisphosphatase and phosphoenolpyruvate synthetase were found to be under the negative control of csrA, and these enzyme activities were maximal during the early stationary phase of growth. The enzymes glucose-6-phosphate isomerase, triose-phosphate isomerase, and enolase were positively regulated by csrA. Thus, csrA exerts reciprocal effects on glycolysis versus gluconeogenesis and glycogen biosynthesis. The glycolytic isozymes pyruvate kinase F and A (encoded by pykF and pykA, respectively) and phosphofructokinase I and II (pfkA and pfkB, respectively) exhibited differential regulation via csrA. Since the individual members of these isozyme pairs are allosterically regulated by different cellular metabolites, csrA is also capable of fine-tuning the allosteric regulation of glycolysis. In contrast, the expression of genes of the pentose phosphate pathway was weakly or negligibly affected by csrA.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J. Timmermans and L. Van Melderen Conditional Essentiality of the csrA Gene in Escherichia coli J. Bacteriol., March 1, 2009; 191(5): 1722 - 1724. [Abstract] [Full Text] [PDF]

				J. A. Fields and S. A. Thompson Campylobacter jejuni CsrA Mediates Oxidative Stress Responses, Biofilm Formation, and Host Cell Invasion J. Bacteriol., May 1, 2008; 190(9): 3411 - 3416. [Abstract] [Full Text] [PDF]

				C. S. Baker, L. A. Eory, H. Yakhnin, J. Mercante, T. Romeo, and P. Babitzke CsrA Inhibits Translation Initiation of Escherichia coli hfq by Binding to a Single Site Overlapping the Shine-Dalgarno Sequence J. Bacteriol., August 1, 2007; 189(15): 5472 - 5481. [Abstract] [Full Text] [PDF]

				J. Deutscher, C. Francke, and P. W. Postma How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria Microbiol. Mol. Biol. Rev., December 1, 2006; 70(4): 939 - 1031. [Abstract] [Full Text] [PDF]

				J. Mercante, K. Suzuki, X. Cheng, P. Babitzke, and T. Romeo Comprehensive Alanine-scanning Mutagenesis of Escherichia coli CsrA Defines Two Subdomains of Critical Functional Importance J. Biol. Chem., October 20, 2006; 281(42): 31832 - 31842. [Abstract] [Full Text] [PDF]

				J. Domka, J. Lee, and T. K. Wood YliH (BssR) and YceP (BssS) Regulate Escherichia coli K-12 Biofilm Formation by Influencing Cell Signaling Appl. Envir. Microbiol., April 1, 2006; 72(4): 2449 - 2459. [Abstract] [Full Text] [PDF]

				A. K. DUBEY, C. S. BAKER, T. ROMEO, and P. BABITZKE RNA sequence and secondary structure participate in high-affinity CsrA-RNA interaction RNA, October 1, 2005; 11(10): 1579 - 1587. [Abstract] [Full Text] [PDF]

				P. Gutierrez, Y. Li, M. J. Osborne, E. Pomerantseva, Q. Liu, and K. Gehring Solution Structure of the Carbon Storage Regulator Protein CsrA from Escherichia coli J. Bacteriol., May 15, 2005; 187(10): 3496 - 3501. [Abstract] [Full Text] [PDF]

				E. L. Murray and T. Conway Multiple Regulators Control Expression of the Entner-Doudoroff Aldolase (Eda) of Escherichia coli J. Bacteriol., February 1, 2005; 187(3): 991 - 1000. [Abstract] [Full Text] [PDF]

				K. Sauer, M. C. Cullen, A. H. Rickard, L. A. H. Zeef, D. G. Davies, and P. Gilbert Characterization of Nutrient-Induced Dispersion in Pseudomonas aeruginosa PAO1 Biofilm J. Bacteriol., November 1, 2004; 186(21): 7312 - 7326. [Abstract] [Full Text] [PDF]

				D. A. Rodionov, M. S. Gelfand, and N. Hugouvieux-Cotte-Pattat Comparative genomics of the KdgR regulon in Erwinia chrysanthemi 3937 and other gamma-proteobacteria Microbiology, November 1, 2004; 150(11): 3571 - 3590. [Abstract] [Full Text] [PDF]

				X. Wang, J. F. Preston III, and T. Romeo The pgaABCD Locus of Escherichia coli Promotes the Synthesis of a Polysaccharide Adhesin Required for Biofilm Formation J. Bacteriol., May 1, 2004; 186(9): 2724 - 2734. [Abstract] [Full Text] [PDF]

				A. K. Dubey, C. S. Baker, K. Suzuki, A. D. Jones, P. Pandit, T. Romeo, and P. Babitzke CsrA Regulates Translation of the Escherichia coli Carbon Starvation Gene, cstA, by Blocking Ribosome Access to the cstA Transcript J. Bacteriol., August 1, 2003; 185(15): 4450 - 4460. [Abstract] [Full Text] [PDF]

				A.-K. Pernestig, D. Georgellis, T. Romeo, K. Suzuki, H. Tomenius, S. Normark, and O. Melefors The Escherichia coli BarA-UvrY Two-Component System Is Needed for Efficient Switching between Glycolytic and Gluconeogenic Carbon Sources J. Bacteriol., February 1, 2003; 185(3): 843 - 853. [Abstract] [Full Text] [PDF]

				K. Suzuki, X. Wang, T. Weilbacher, A.-K. Pernestig, O. Melefors, D. Georgellis, P. Babitzke, and T. Romeo Regulatory Circuitry of the CsrA/CsrB and BarA/UvrY Systems of Escherichia coli J. Bacteriol., September 15, 2002; 184(18): 5130 - 5140. [Abstract] [Full Text] [PDF]

				D. W. Jackson, J. W. Simecka, and T. Romeo Catabolite Repression of Escherichia coli Biofilm Formation J. Bacteriol., June 15, 2002; 184(12): 3406 - 3410. [Abstract] [Full Text] [PDF]

				D. W. Jackson, K. Suzuki, L. Oakford, J. W. Simecka, M. E. Hart, and T. Romeo Biofilm Formation and Dispersal under the Influence of the Global Regulator CsrA of Escherichia coli J. Bacteriol., January 1, 2002; 184(1): 290 - 301. [Abstract] [Full Text] [PDF]

				S. Gudapaty, K. Suzuki, X. Wang, P. Babitzke, and T. Romeo Regulatory Interactions of Csr Components: the RNA Binding Protein CsrA Activates csrB Transcription in Escherichia coli J. Bacteriol., October 15, 2001; 183(20): 6017 - 6027. [Abstract] [Full Text] [PDF]

				S. GOTTESMAN, G. STORZ, C. ROSENOW, N. MAJDALANI, F. REPOILA, and K.M. WASSARMAN Small RNA Regulators of Translation: Mechanisms of Action and Approaches for Identifying New Small RNAs Cold Spring Harb Symp Quant Biol, January 1, 2001; 66(0): 353 - 362. [Abstract] [PDF]

				B. Wei, S. Shin, D. LaPorte, A. J. Wolfe, and T. Romeo Global Regulatory Mutations in csrA and rpoS Cause Severe Central Carbon Stress in Escherichia coli in the Presence of Acetate J. Bacteriol., March 15, 2000; 182(6): 1632 - 1640. [Abstract] [Full Text]

				M. K. B. Berlyn Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984. [Abstract] [Full Text] [PDF]

				M. Y. Liu, G. Gui, B. Wei, J. F. Preston III, L. Oakford, U. Yuksel, D. P. Giedroc, and T. Romeo The RNA Molecule CsrB Binds to the Global Regulatory Protein CsrA and Antagonizes Its Activity in Escherichia coli J. Biol. Chem., July 11, 1997; 272(28): 17502 - 17510. [Abstract] [Full Text] [PDF]