Ca mobilization from intracellular stores is a major event in the signaling cascade triggered by peptide hormone receptors. The transient rise in intracellular free Ca concentration ([Ca]) is well characterized, but little is known about alterations of total cell Ca. Therefore we established a technique to determine changes in total cell Ca during hormone stimulation of Ca-loaded cells. Bradykinin and endothelin-1 reduced total cell Ca by up to 56% in HF-15 cells, COS-7 cells, and CHO K1 cells transfected with the rat B2 receptor cDNA. In Rat-1 cells and PC-12 cells, stimulation with endothelin-1 or bradykinin did not result in a net decrease in total cell Ca at physiological extracellular Ca concentration. Decrease in total cell Ca was preceded by an increase in [Ca] and blunting of the transient rise in [Ca] by a Ca chelator prevented the hormone-induced decrease in total cell Ca. Previous reduction of total cell Ca by one hormone suppressed the transient rise in [Ca] induced by another. The data present evidence that the hormones bradykinin and endothelin-1 are capable of switching off the Ca-mobilizing signal transduction pathway in a cell by depleting intracellular Ca stores. This process is accompanied by a significant reduction of total cell Ca.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				G. Tamma, M. Carmosino, M. Svelto, and G. Valenti Bradykinin Signaling Counteracts cAMP-Elicited Aquaporin 2 Translocation in Renal Cells J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2881 - 2889. [Abstract] [Full Text] [PDF]

				J. Scharfstein, V. Schmitz, V. Morandi, M. M. A. Capella, A. P. C. A. Lima, A. Morrot, L. Juliano, and W. Muller-Esterl Host cell invasion by Trypanosoma cruzi is potentiated by activation of bradykinin B2 receptors J. Exp. Med., November 6, 2000; 192(9): 1289 - 1300. [Abstract] [Full Text] [PDF]

				S. AbdAlla, E. Zaki, H. Lother, and U. Quitterer Involvement of the Amino Terminus of the B2 Receptor in Agonist-induced Receptor Dimerization J. Biol. Chem., September 10, 1999; 274(37): 26079 - 26084. [Abstract] [Full Text] [PDF]

				U. Quitterer, E. Zaki, and S. AbdAlla Investigation of the Extracellular Accessibility of the Connecting Loop between Membrane Domains I and II of the Bradykinin B2 Receptor J. Biol. Chem., May 21, 1999; 274(21): 14773 - 14778. [Abstract] [Full Text] [PDF]

				V. Soskic, E. Nyakatura, M. Roos, W. Muller-Esterl, and J. Godovac-Zimmermann Correlations in Palmitoylation and Multiple Phosphorylation of Rat Bradykinin B2 Receptor in Chinese Hamster Ovary Cells J. Biol. Chem., March 26, 1999; 274(13): 8539 - 8545. [Abstract] [Full Text] [PDF]

				A. Pizard, J. Marchetti, J. Allegrini, F. Alhenc-Gelas, and R. M. Rajerison Negative Cooperativity in the Human Bradykinin B2 Receptor J. Biol. Chem., January 16, 1998; 273(3): 1309 - 1315. [Abstract] [Full Text] [PDF]

				A. Blaukat, S. A. Alla, M. J. Lohse, and W. Muller-Esterl Ligand-induced Phosphorylation/Dephosphorylation of the Endogenous Bradykinin B2 Receptor from Human Fibroblasts J. Biol. Chem., December 13, 1996; 271(50): 32366 - 32374. [Abstract] [Full Text] [PDF]

				S. AbdAlla, K. Jarnagin, W. Muller-Esterl, and U. Quitterer The N-terminal Amino Group of [Tyr8]Bradykinin Is Bound Adjacent to Analogous Amino Acids of the Human and Rat B2 Receptor J. Biol. Chem., November 1, 1996; 271(44): 27382 - 27387. [Abstract] [Full Text] [PDF]

				A. Horstmeyer, H. Cramer, T. Sauer, W. Muller-Esterl, and C. Schroeder Palmitoylation of Endothelin Receptor A. DIFFERENTIAL MODULATION OF SIGNAL TRANSDUCTION ACTIVITY BY POST-TRANSLATIONAL MODIFICATION J. Biol. Chem., August 23, 1996; 271(34): 20811 - 20819. [Abstract] [Full Text] [PDF]

				S. A. Alla, U. Quitterer, S. Grigoriev, A. Maidhof, M. Haasemann, K. Jarnagin, and W. Müller-Esterl Extracellular Domains of the Bradykinin B2 Receptor Involved in Ligand Binding and Agonist Sensing Defined by Anti-peptide Antibodies J. Biol. Chem., January 19, 1996; 271(3): 1748 - 1755. [Abstract] [Full Text] [PDF]