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(Received for publication, August 19, 1994; and in revised form, November
3, 1994) Genomic and cDNA clones encoding the chicken neuronal nicotinic
acetylcholine receptor
Volume 270,
Number 7,
Issue of February 17, 1995 pp. 3224-3233
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
3 Gene
ITS REGULATION WITHIN THE AVIAN NERVOUS SYSTEM IS EFFECTED BY A
PROMOTER 143 BASE PAIRS IN LENGTH
3 subunit were isolated and sequenced. The
3 gene consists of six protein-encoding exons and the deduced
protein has the structural features found in all other members of the
neuronal nicotinic acetylcholine receptor subunit family. Although they
are undetectable in most brain compartments, 3 mRNAs are
relatively abundant in the developing retina and in the trigeminal
ganglion. In situ hybridization and immunohistochemical
analysis demonstrated that in retina, 3 transcripts and protein
are confined to subpopulations of cells in the inner nuclear and
ganglion cell layers. 3 is expressed in the proximal and distal
regions of the developing trigeminal ganglion, i.e. in both
placode- and neural crest-derived neurons. Transient transfection
assays in cells freshly dissociated from selected regions of the
central nervous system at different developmental stages allowed the
identification of genetic elements involved in the neuronal-selective
expression of the 3 gene. A promoter fragment 143 base pairs in
length and containing TATA, CAAT, and other consensus sequences is
sufficient to restrict reporter gene expression to a subpopulation of
retinal neurons. This promoter is totally inactive upon transfection
into neuronal and non-neuronal cells from other regions of the central
nervous system.
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