We showed previously that the alternative splicing of chondroitin sulfate attachment domains (CS and CS ) yielded multiforms of the PG-M core protein in mouse. A transcript encoding a new short form of the core protein PG-M(V3) was found in various mouse tissues using polymerase chain reaction. DNA sequences of the polymerase chain reaction products suggested that PG-M(V3) had no chondroitin sulfate attachment domain. PG-M(V3) was also detected in various human tissues. The presence of a transcript for PG-M(V3) was further supported by Northern blot analysis. Southern blot analysis confirmed that multiforms of the PG-M core protein, including PG-M(V3), were derived from a single genomic locus by an alternative splicing mechanism. Because PG-M(V3) has no chondroitin sulfate attachment region, which is the most distinctive portion of a proteoglycan molecule, this form may have a unique function.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J Kodama, Hasengaowa, T Kusumoto, N Seki, T Matsuo, Y Ojima, K Nakamura, A Hongo, and Y Hiramatsu Prognostic significance of stromal versican expression in human endometrial cancer Ann. Onc., February 1, 2007; 18(2): 269 - 274. [Abstract] [Full Text] [PDF]

				S. Dutt, M. Kleber, M. Matasci, L. Sommer, and D. R. Zimmermann Versican V0 and V1 Guide Migratory Neural Crest Cells J. Biol. Chem., April 28, 2006; 281(17): 12123 - 12131. [Abstract] [Full Text] [PDF]

				Y. Inoue, M. Yoneda, J. Zhao, O. Miyaishi, A. Ohno-Jinno, T. Kataoka, Z. Isogai, K. Kimata, M. Iwaki, and M. Zako Molecular Cloning and Characterization of Chick SPACRCAN J. Biol. Chem., April 14, 2006; 281(15): 10381 - 10388. [Abstract] [Full Text] [PDF]

				N. Kamiya, H. Watanabe, H. Habuchi, H. Takagi, T. Shinomura, K. Shimizu, and K. Kimata Versican/PG-M Regulates Chondrogenesis as an Extracellular Matrix Molecule Crucial for Mesenchymal Condensation J. Biol. Chem., January 27, 2006; 281(4): 2390 - 2400. [Abstract] [Full Text] [PDF]

				M. Nakamura, S. Sone, I. Takahashi, I. Mizoguchi, S. Echigo, and Y. Sasano Expression of Versican and ADAMTS1, 4, and 5 During Bone Development in the Rat Mandible and Hind Limb J. Histochem. Cytochem., December 1, 2005; 53(12): 1553 - 1562. [Abstract] [Full Text] [PDF]

				T. Miyamoto, H. Inoue, Y. Sakamoto, E. Kudo, T. Naito, T. Mikawa, Y. Mikawa, Y. Isashiki, D. Osabe, S. Shinohara, et al. Identification of a Novel Splice Site Mutation of the CSPG2 Gene in a Japanese Family with Wagner Syndrome Invest. Ophthalmol. Vis. Sci., August 1, 2005; 46(8): 2726 - 2735. [Abstract] [Full Text] [PDF]

				A. Hinek, K. R. Braun, K. Liu, Y. Wang, and T. N. Wight Retrovirally Mediated Overexpression of Versican V3 Reverses Impaired Elastogenesis and Heightened Proliferation Exhibited by Fibroblasts from Costello Syndrome and Hurler Disease Patients Am. J. Pathol., January 1, 2004; 164(1): 119 - 131. [Abstract] [Full Text] [PDF]

				Y. Zhang, U. Rauch, and M.-T. R. Perez Accumulation of Neurocan, a Brain Chondroitin Sulfate Proteoglycan, in Association with the Retinal Vasculature in RCS Rats Invest. Ophthalmol. Vis. Sci., March 1, 2003; 44(3): 1252 - 1261. [Abstract] [Full Text] [PDF]

				D. L. Russell, S. A. Ochsner, M. Hsieh, S. Mulders, and J. S. Richards Hormone-Regulated Expression and Localization of Versican in the Rodent Ovary Endocrinology, March 1, 2003; 144(3): 1020 - 1031. [Abstract] [Full Text] [PDF]

				S. Cattaruzza, M. Schiappacassi, A. Ljungberg-Rose, P. Spessotto, D. Perissinotto, M. Morgelin, M. T. Mucignat, A. Colombatti, and R. Perris Distribution of PG-M/Versican Variants in Human Tissues and de Novo Expression of Isoform V3 upon Endothelial Cell Activation, Migration, and Neoangiogenesis in Vitro J. Biol. Chem., November 27, 2002; 277(49): 47626 - 47635. [Abstract] [Full Text] [PDF]

				M. Zako, M. Iwaki, M. Yoneda, O. Miyaishi, J. Zhao, Y. Suzuki, M. Takeuchi, G. Miyake, H. Ikagawa, and K. Kimata Molecular Cloning and Characterization of Chick Sialoprotein Associated with Cones and Rods, a Developmentally Regulated Glycoprotein of Interphotoreceptor Matrix J. Biol. Chem., July 5, 2002; 277(28): 25592 - 25600. [Abstract] [Full Text] [PDF]

				H. Kawashima, K. Atarashi, M. Hirose, J. Hirose, S. Yamada, K. Sugahara, and M. Miyasaka Oversulfated Chondroitin/Dermatan Sulfates Containing GlcAbeta 1/IdoAalpha 1-3GalNAc(4,6-O-disulfate) Interact with L- and P-selectin and Chemokines J. Biol. Chem., April 5, 2002; 277(15): 12921 - 12930. [Abstract] [Full Text] [PDF]

				M. J. Merrilees, J. M. Lemire, J. W. Fischer, M. G. Kinsella, K. R. Braun, A. W. Clowes, and T. N. Wight Retrovirally Mediated Overexpression of Versican V3 by Arterial Smooth Muscle Cells Induces Tropoelastin Synthesis and Elastic Fiber Formation In Vitro and In Neointima After Vascular Injury Circ. Res., March 8, 2002; 90(4): 481 - 487. [Abstract] [Full Text] [PDF]

				Z. Isogai, A. Aspberg, D. R. Keene, R. N. Ono, D. P. Reinhardt, and L. Y. Sakai Versican Interacts with Fibrillin-1 and Links Extracellular Microfibrils to Other Connective Tissue Networks J. Biol. Chem., February 1, 2002; 277(6): 4565 - 4572. [Abstract] [Full Text] [PDF]

				C. E. Bandtlow and D. R. Zimmermann Proteoglycans in the Developing Brain: New Conceptual Insights for Old Proteins Physiol Rev, October 1, 2000; 80(4): 1267 - 1290. [Abstract] [Full Text] [PDF]

				D Perissinotto, P Iacopetti, I Bellina, R Doliana, A Colombatti, Z Pettway, M Bronner-Fraser, T Shinomura, K Kimata, M Morgelin, et al. Avian neural crest cell migration is diversely regulated by the two major hyaluronan-binding proteoglycans PG-M/versican and aggrecan Development, January 7, 2000; 127(13): 2823 - 2842. [Abstract] [PDF]

				M Schmalfeldt, C. Bandtlow, M. Dours-Zimmermann, K. Winterhalter, and D. Zimmermann Brain derived versican V2 is a potent inhibitor of axonal growth J. Cell Sci., January 3, 2000; 113(5): 807 - 816. [Abstract] [PDF]

				J. M. Lemire, K. R. Braun, P. Maurel, E. D. Kaplan, S. M. Schwartz, and T. N. Wight Versican/PG-M Isoforms in Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., July 1, 1999; 19(7): 1630 - 1639. [Abstract] [Full Text] [PDF]

				J. Kishimoto, R. Ehama, L. Wu, S. Jiang, N. Jiang, and R. E. Burgeson Selective activation of the versican promoter by epithelial- mesenchymal interactions during hair follicle development PNAS, June 22, 1999; 96(13): 7336 - 7341. [Abstract] [Full Text] [PDF]

				H. Kawashima, Y.-F. Li, N. Watanabe, J. Hirose, M. Hirose, and M. Miyasaka Identification and characterization of ligands for L-selectin in the kidney. I. Versican, a large chondroitin sulfate proteoglycan, is a ligand for L-selectin Int. Immunol., March 1, 1999; 11(3): 393 - 405. [Abstract] [Full Text] [PDF]

				M. Schmalfeldt, M. T. Dours-Zimmermann, K. H. Winterhalter, and D. R. Zimmermann Versican V2 Is a Major Extracellular Matrix Component of the Mature Bovine Brain J. Biol. Chem., June 19, 1998; 273(25): 15758 - 15764. [Abstract] [Full Text] [PDF]

				M. Zako, T. Shinomura, and K. Kimata Alternative Splicing of the Unique "PLUS" Domain of Chicken PG-M/Versican Is Developmentally Regulated J. Biol. Chem., April 4, 1997; 272(14): 9325 - 9331. [Abstract] [Full Text] [PDF]

				J. M. Lemire, S. Potter-Perigo, K. L. Hall, T. N. Wight, and S. M. Schwartz Distinct Rat Aortic Smooth Muscle Cells Differ in Versican/PG-M Expression Arterioscler. Thromb. Vasc. Biol., June 1, 1996; 16(6): 821 - 829. [Abstract] [Full Text]

				T. Shinomura, M. Zako, K. Ito, M. Ujita, and K. Kimata The Gene Structure and Organization of Mouse PG-M, a Large Chondroitin Sulfate Proteoglycan J. Biol. Chem., April 28, 1995; 270(17): 10328 - 10333. [Abstract] [Full Text] [PDF]

				B. L. Yang, L. Cao, C. Kiani, V. Lee, Y. Zhang, M. E. Adams, and B. B. Yang Tandem Repeats Are Involved in G1 Domain Inhibition of Versican Expression and Secretion and the G3 Domain Enhances Glycosaminoglycan Modification and Product Secretion via the Complement-binding Protein-like Motif J. Biol. Chem., July 7, 2000; 275(28): 21255 - 21261. [Abstract] [Full Text] [PDF]

				H. Kawashima, M. Hirose, J. Hirose, D. Nagakubo, A. H. K. Plaas, and M. Miyasaka Binding of a Large Chondroitin Sulfate/Dermatan Sulfate Proteoglycan, Versican, to L-selectin, P-selectin, and CD44 J. Biol. Chem., November 3, 2000; 275(45): 35448 - 35456. [Abstract] [Full Text] [PDF]

				J. D. Sandy, J. Westling, R. D. Kenagy, M. L. Iruela-Arispe, C. Verscharen, J. C. Rodriguez-Mazaneque, D. R. Zimmermann, J. M. Lemire, J. W. Fischer, T. N. Wight, et al. Versican V1 Proteolysis in Human Aorta in Vivo Occurs at the Glu441-Ala442 Bond, a Site That Is Cleaved by Recombinant ADAMTS-1 and ADAMTS-4 J. Biol. Chem., April 13, 2001; 276(16): 13372 - 13378. [Abstract] [Full Text] [PDF]

				M. J. Merrilees, J. M. Lemire, J. W. Fischer, M. G. Kinsella, K. R. Braun, A. W. Clowes, and T. N. Wight Retrovirally Mediated Overexpression of Versican V3 by Arterial Smooth Muscle Cells Induces Tropoelastin Synthesis and Elastic Fiber Formation In Vitro and In Neointima After Vascular Injury Circ. Res., March 8, 2002; 90(4): 481 - 487. [Abstract] [Full Text] [PDF]