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(Received for publication, October 24, 1994; and in revised form, January 3, 1995) Two ryanodine receptor (RyR), sarcoplasmic reticulum
Ca
Volume 270,
Number 9,
Issue of March 3, 1995 pp. 4220-4223
©1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Ryanodine Receptor
IMPLICATIONS FOR A TWO-RYANODINE RECEPTOR SYSTEM
release channels, 
and 
, co-exist in
chicken skeletal muscles. To investigate a two-RyR Ca release system, we compared electrically evoked Ca transients in Crooked Neck Dwarf (cn/cn) cultured muscle cells,
which do not make RyR, and normal (+/?) cells. At day 3 in
culture, Ca release in +/? cells required
extracellular Ca (Ca
), and Ca transients had slow kinetics. At day 5, Ca release was Ca-independent
in 40% of the cells, and transients were more rapid. By day 7, all
+/? cells had
Ca-independent Ca release. Contractions were observed in +/? cells on all
days. Ca transients were observed in cn/cn cells on
days 3, 5, and 7, but in each case they were
Ca-dependent and exhibited slow
kinetics. Localized vesiculations, not contractions, occurred in cn/cn
cells. By day 10, Ca transients were no longer
observed in cn/cn cells even in
Ca. Sarcoplasmic reticulum
Ca was not depleted, as caffeine induced
Ca transients. Thus, in the absence of RyR there
is a failure to develop
Ca-independent Ca release and contractions and to sustain
Ca-dependent release. Moreover,
contributions by the RyR cannot be duplicated by the RyR
alone.
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