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AFFECTS GROWTH ON ORGANIC NITROGEN
AND THE CONDITIONAL LETHALITY OF AN era
MUTANT
(Received for publication, September 7, 1994)
Two rpoN-linked 
Tn10-kan insertions
suppress the conditionally lethal era allele. One
truncates rpoN while the second disrupts another gene (ptsN) in the rpoN operon and does not affect
classical nitrogen regulation. Neither alter expression of era indicating that suppression is post-translational. Plasmid clones
of ptsN prevent suppression by either disruption mutation
indicating that this gene is important for lethality caused by era. rpoN and six neighboring genes were
sequenced and compared with sequences in the database. Two of these
genes encode proteins homologous to Enzyme IIA
and HPr of
the phosphoenolpyruvate:sugar phosphotransferase system. We designate
these proteins IIA (ptsN) and NPr (npr). Purified IIA and NPr exchange phosphate
appropriately with Enzyme I, HPr, and Enzyme IIA proteins of the
phosphoenolpyruvate:sugar phosphotransferase system. Several sugars and
tricarboxylic acid cycle intermediates inhibited growth of the ptsN disruption mutant on medium containing an amino acid or nucleoside
base as a combined source of nitrogen, carbon, and energy. This growth
inhibition was relieved by supplying the ptsN gene or ammonium
salts but was not aleviated by altering levels of exogenously supplied
cAMP. These results support our previous proposal of a novel mechanism
linking carbon and nitrogen assimilation and relates IIA to the unknown process regulated by the essential GTPase Era.
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