| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, June 7, 1995; and in revised form, October 2, 1995)
RalGDS is a GDP/GTP exchange protein for ral p24, a
member of small GTP-binding protein superfamily. We have recently shown
that RalGDS interacts directly with the GTP-bound active form of ras p21 through the effector loop of ras p21 in
vitro, in insect cells and in the yeast two-hybrid system. These
results suggest that RalGDS functions as an effector protein of ras p21. Here, we report that RalGDS interacts with ras p21
in mammalian cells in response to an extracellular signal. Epidermal
growth factor (EGF) induced the interaction of c-ras p21 and
RalGDS in COS cells expressing both proteins, but not in the cells
expressing RalGDS and c-ras p21, which is an
effector loop mutant of ras p21. We also found that cyclic
AMP-dependent protein kinase (protein kinase A) regulated the
selectivity of ras p21-binding to either RalGDS or Raf-1.
Protein kinase A phosphorylated RalGDS as well as (1-149)Raf
(amino acid residues 1-149). Although the phosphorylated
(1-149)Raf had a lower affinity for ras p21 than the
unphosphorylated (1-149)Raf, both the phosphorylated and
unphosphorylated RalGDS had the similar affinities for ras p21. The phosphorylation of RalGDS did not affect its activity to
stimulate the GDP/GTP exchange of ral p24. Pretreatment of COS
cells with forskolin further stimulated the interaction of ras p21 and RalGDS induced by EGF under the conditions that
EGF-dependent Raf-1 activity was inhibited. These results indicate that ras p21 distinguishes between RalGDS and Raf-1 by their
phosphorylation by protein kinase A.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. G. Rondaij, R. Bierings, E. L. van Agtmaal, K. A. Gijzen, E. Sellink, A. Kragt, S. S. G. Ferguson, K. Mertens, M. J. Hannah, J. A. van Mourik, et al. Guanine exchange factor RalGDS mediates exocytosis of Weibel-Palade bodies from endothelial cells Blood, July 1, 2008; 112(1): 56 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Ahmed, D. Gesty-Palmer, M. K. Drezner, and L. M. Luttrell Transactivation of the Epidermal Growth Factor Receptor Mediates Parathyroid Hormone and Prostaglandin F2{alpha}-Stimulated Mitogen-Activated Protein Kinase Activation in Cultured Transgenic Murine Osteoblasts Mol. Endocrinol., August 1, 2003; 17(8): 1607 - 1621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Feldman, A. M. Zamah, K. L. Pierce, W. E. Miller, F. Kelly, A. Rapacciuolo, H. A. Rockman, W. J. Koch, and L. M. Luttrell Selective Inhibition of Heterotrimeric Gs Signaling. TARGETING THE RECEPTOR-G PROTEIN INTERFACE USING A PEPTIDE MINIGENE ENCODING THE Galpha s CARBOXYL TERMINUS J. Biol. Chem., August 2, 2002; 277(32): 28631 - 28640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Clough, R. S. Sidhu, and R. P. Bhullar Calmodulin Binds RalA and RalB and Is Required for the Thrombin-induced Activation of Ral in Human Platelets J. Biol. Chem., August 2, 2002; 277(32): 28972 - 28980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Pursiheimo, J. Saari, M. Jalkanen, and M. Salmivirta Cooperation of Protein Kinase A and Ras/ERK Signaling Pathways Is Required for AP-1-mediated Activation of Fibroblast Growth Factor-inducible Response Element (FiRE) J. Biol. Chem., July 5, 2002; 277(28): 25344 - 25355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Schmitt and P. J. S. Stork Cyclic AMP-Mediated Inhibition of Cell Growth Requires the Small G Protein Rap1 Mol. Cell. Biol., June 1, 2001; 21(11): 3671 - 3683. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Pearson, F. Robinson, T. Beers Gibson, B.-e Xu, M. Karandikar, K. Berman, and M. H. Cobb Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions Endocr. Rev., April 1, 2001; 22(2): 153 - 183. [Abstract] [Full Text]
|
|
|
|
|
|
O. M. Tsygankova, A. Saavedra, J. F. Rebhun, L. A. Quilliam, and J. L. Meinkoth Coordinated Regulation of Rap1 and Thyroid Differentiation by Cyclic AMP and Protein Kinase A Mol. Cell. Biol., March 15, 2001; 21(6): 1921 - 1929. [Abstract] [Full Text]
|
|
|
|
|
|
T. Andoh, Y. Hirata, and A. Kikuchi Yeast Glycogen Synthase Kinase 3 Is Involved in Protein Degradation in Cooperation with Bul1, Bul2, and Rsp5 Mol. Cell. Biol., September 15, 2000; 20(18): 6712 - 6720. [Abstract] [Full Text]
|
|
|
|
|
|
C. Giglione and A. Parmeggiani Raf-1 Is Involved in the Regulation of the Interaction between Guanine Nucleotide Exchange Factor and Ha-Ras. EVIDENCES FOR A FUNCTION OF Raf-1 AND PHOSPHATIDYLINOSITOL 3-KINASE UPSTREAM TO Ras J. Biol. Chem., December 25, 1998; 273(52): 34737 - 34744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Miller, L. Rioux, G. V. Prendergast, S. Cannon, M. A. White, and J. L. Meinkoth Differential Effects of Protein Kinase A on Ras Effector Pathways Mol. Cell. Biol., July 1, 1998; 18(7): 3718 - 3726. [Abstract] [Full Text]
|
|
|
|
|
|
H. Yamamoto, S. Kishida, T. Uochi, S. Ikeda, S. Koyama, M. Asashima, and A. Kikuchi Axil, a Member of the Axin Family, Interacts with Both Glycogen Synthase Kinase 3beta and beta -Catenin and Inhibits Axis Formation of Xenopus Embryos Mol. Cell. Biol., May 1, 1998; 18(5): 2867 - 2875. [Abstract] [Full Text]
|
|
|
|
|
|
S. Kishida, H. Yamamoto, S. Ikeda, M. Kishida, I. Sakamoto, S. Koyama, and A. Kikuchi Axin, a Negative Regulator of the Wnt Signaling Pathway, Directly Interacts with Adenomatous Polyposis Coli and Regulates the Stabilization of beta -Catenin J. Biol. Chem., May 1, 1998; 273(18): 10823 - 10826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ikeda, O. Ishida, T. Hinoi, S. Kishida, and A. Kikuchi Identification and Characterization of a Novel Protein Interacting with Ral-binding Protein 1, a Putative Effector Protein of Ral J. Biol. Chem., January 9, 1998; 273(2): 814 - 821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Wang, M. T. Khan, and B. D. Roufogalis Identification and Characterization of a Calmodulin-binding Domain in Ral-A, a Ras-related GTP-binding Protein Purified from Human Erythrocyte Membrane J. Biol. Chem., June 20, 1997; 272(25): 16002 - 16009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Murai, M. Ikeda, S. Kishida, O. Ishida, M. Okazaki-Kishida, Y. Matsuura, and A. Kikuchi Characterization of Ral GDP Dissociation Stimulator-like (RGL) Activities to Regulate c-fos Promoter and the GDP/GTP Exchange of Ral J. Biol. Chem., April 18, 1997; 272(16): 10483 - 10490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Miller, S. Prigent, E. Kupperman, L. Rioux, S.-H. Park, J. R. Feramisco, M. A. White, J. L. Rutkowski, and J. L. Meinkoth RalGDS Functions in Ras- and cAMP-mediated Growth Stimulation J. Biol. Chem., February 28, 1997; 272(9): 5600 - 5605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hinoi, S. Kishida, S. Koyama, M. Ikeda, Y. Matsuura, and A. Kikuchi Post-translational Modifications of Ras and Ral Are Important for the Action of Ral GDP Dissociation Stimulator J. Biol. Chem., August 16, 1996; 271(33): 19710 - 19716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. White, T. Vale, J. H. Camonis, E. Schaefer, and M. H. Wigler A Role for the Ral Guanine Nucleotide Dissociation Stimulator in Mediating Ras-induced Transformation J. Biol. Chem., July 12, 1996; 271(28): 16439 - 16442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Booden, D. S. Sakaguchi, and J. E. Buss Mutation of Ha-Ras C Terminus Changes Effector Pathway Utilization J. Biol. Chem., July 28, 2000; 275(31): 23559 - 23568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Qiu, S. Zhuang, F. C. von Lintig, G. R. Boss, and R. B. Pilz Cell Type-specific Regulation of B-Raf Kinase by cAMP and 14-3-3 Proteins J. Biol. Chem., October 6, 2000; 275(41): 31921 - 31929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hinoi, H. Yamamoto, M. Kishida, S. Takada, S. Kishida, and A. Kikuchi Complex Formation of Adenomatous Polyposis Coli Gene Product and Axin Facilitates Glycogen Synthase Kinase-3beta -dependent Phosphorylation of beta -Catenin and Down-regulates beta -Catenin J. Biol. Chem., October 27, 2000; 275(44): 34399 - 34406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yamamoto, T. Hinoi, T. Michiue, A. Fukui, H. Usui, V. Janssens, C. Van Hoof, J. Goris, M. Asashima, and A. Kikuchi Inhibition of the Wnt Signaling Pathway by the PR61 Subunit of Protein Phosphatase 2A J. Biol. Chem., July 13, 2001; 276(29): 26875 - 26882. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
