Regulation of the cytosolic free Ca concentration by nerve growth factor was investigated in C6-2B glioma cells newly expressing the high affinity nerve growth factor receptor trkA, using Fura-2 fluorescence ratio imaging. In these cells, nerve growth factor (50 ng/ml) evoked a novel 3-fold increase in cytosolic free Ca concentration, while no measurable Ca response was observed in wild type or mock-transfected cells lacking a functional trkA receptor. K-252a, a tyrosine kinase inhibitor which prevents nerve growth factor-mediated responses in C6-2B cells expressing trkA, also blocked the rise in cytosolic free Ca concentration by nerve growth factor. Moreover, basic fibroblast growth factor, which in these cells elicits biochemical changes similar to nerve growth factor, failed to affect cytosolic free Ca concentration, further supporting the specificity of nerve growth factor/trkA receptor in mediating a Ca response. While insensitive to chelation of extracellular Ca, the response was abolished following depletion of Ca stores or blockade of intracellular Ca release, providing strong evidence that intracellular Ca is the main source for nerve growth factor-evoked cytosolic free Ca concentration increase. Nerve growth factor increased the cytosolic free Ca concentration also in NIH3T3 cells overexpressing trkA but devoid of p75 nerve growth factor receptor. Our data suggest that trkA but not p75 is required for nerve growth factor-evoked Ca signaling.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. J. Perez-Garcia, M. Gou-Fabregas, Y. de Pablo, M. Llovera, J. X. Comella, and R. M. Soler Neuroprotection by Neurotrophic Factors and Membrane Depolarization Is Regulated by Calmodulin Kinase IV J. Biol. Chem., February 15, 2008; 283(7): 4133 - 4144. [Abstract] [Full Text] [PDF]

				M. J. Perez-Garcia, V. Cena, Y. de Pablo, M. Llovera, J. X. Comella, and R. M. Soler Glial Cell Line-derived Neurotrophic Factor Increases Intracellular Calcium Concentration: ROLE OF CALCIUM/CALMODULIN IN THE ACTIVATION OF THE PHOSPHATIDYLINOSITOL 3-KINASE PATHWAY J. Biol. Chem., February 13, 2004; 279(7): 6132 - 6142. [Abstract] [Full Text] [PDF]

				A. Bachis, A. M. Colangelo, S. Vicini, P. P. Doe, M. A. De Bernardi, G. Brooker, and I. Mocchetti Interleukin-10 Prevents Glutamate-Mediated Cerebellar Granule Cell Death by Blocking Caspase-3-Like Activity J. Neurosci., May 1, 2001; 21(9): 3104 - 3112. [Abstract] [Full Text] [PDF]

				G. M. de Plater, P. J. Milburn, and R. L. Martin Venom From the Platypus, Ornithorhynchus anatinus, Induces a Calcium-Dependent Current in Cultured Dorsal Root Ganglion Cells J Neurophysiol, March 1, 2001; 85(3): 1340 - 1345. [Abstract] [Full Text] [PDF]

				J. Egea, C. Espinet, R. M. Soler, S. Peiró, N. Rocamora, and J. X. Comella Nerve Growth Factor Activation of the Extracellular Signal-Regulated Kinase Pathway Is Modulated by Ca2+ and Calmodulin Mol. Cell. Biol., March 15, 2000; 20(6): 1931 - 1946. [Abstract] [Full Text]

				H. Jiang, K. Takeda, P. Lazarovici, Y. Katagiri, Z.-X. Yu, G. Dickens, A. Chabuk, X.-W. Liu, V. Ferrans, and G. Guroff Nerve Growth Factor (NGF)-induced Calcium Influx and Intracellular Calcium Mobilization in 3T3 Cells Expressing NGF Receptors J. Biol. Chem., September 10, 1999; 274(37): 26209 - 26216. [Abstract] [Full Text] [PDF]

				C. Brandoli, A. Sanna, M. A. De Bernardi, P. Follesa, G. Brooker, and I. Mocchetti Brain-Derived Neurotrophic Factor and Basic Fibroblast Growth Factor Downregulate NMDA Receptor Function in Cerebellar Granule Cells J. Neurosci., October 1, 1998; 18(19): 7953 - 7961. [Abstract] [Full Text] [PDF]

				A. Kruttgen, J. C. Moller, J. V. Heymach Jr., and E. M. Shooter Neurotrophins induce release of neurotrophins by the regulated secretory pathway PNAS, August 4, 1998; 95(16): 9614 - 9619. [Abstract] [Full Text] [PDF]

				G. Gallo and P. C. Letourneau Localized Sources of Neurotrophins Initiate Axon Collateral Sprouting J. Neurosci., July 15, 1998; 18(14): 5403 - 5414. [Abstract] [Full Text] [PDF]

				Q. Wang and J. Q. Zheng cAMP-Mediated Regulation of Neurotrophin-Induced Collapse of Nerve Growth Cones J. Neurosci., July 1, 1998; 18(13): 4973 - 4984. [Abstract] [Full Text] [PDF]

				G. Gallo, F. B. Lefcort, and P. C. Letourneau The trkA Receptor Mediates Growth Cone Turning toward a Localized Source of Nerve Growth Factor J. Neurosci., July 15, 1997; 17(14): 5445 - 5454. [Abstract] [Full Text] [PDF]