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(Received for publication, September 21, 1995) We report the identification of the gene encoding a DNA
photolyase (phrA) from the Gram-negative eubacterium Myxococcus xanthus. The deduced amino acid sequence of M.
xanthus photolyase indicates that the protein contains 401 amino
acids (M
Volume 271,
Number 11,
Issue of March 15, 1996 pp. 6252-6259
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Higher
Eukaryotes than
to Photolyases of Other Eubacteria
45,071). By comparison of the amino acid
and DNA sequences with those of other known photolyases, it has been
found that it is more similar to the deduced amino acid sequences of
the photolyases of ``higher'' eukaryotes than to the
photolyases of other eubacteria. Recombinant plasmids carrying M.
xanthus phrA rescue the photoreactivation activity of an
irradiated strain of Escherichia coli with a deletion in phrA. This rescue is light-dependent.
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