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Volume 271, Number 16, Issue of April 19, 1996 pp. 9259-9266
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Existence of Novel Branched Side Chains Containing -1,2 and -1,6 Linkages Corresponding to Antigenic Factor 9 in the Mannan of Candida guilliermondii

(Received for publication, November 3, 1995; and in revised form, January 30, 1996)

Nobuyuki Shibata ,  Rieko Akagi ,  Tomoko Hosoya ,  Kumi Kawahara ,  Akifumi Suzuki ,  Kyoko Ikuta ,  Hidemitsu Kobayashi ,  Kanehiko Hisamichi ,  Yoshio Okawa ,  Shigeo Suzuki

Isolation of beta-linkage-containing side chain oligosaccharides from the mannan of Candida gilliermondii IFO 10279 strain has been conducted by acetolysis under mild conditions. A structural study of these oligosaccharides by one- and two-dimensional NMR and methylation analyses indicated the presence of extended oligosaccharide side chains with two consecutive beta-1,2-linked mannose units at the nonreducing terminal of alpha-linked oligosaccharides. The linkage sequence present in this mannan, Manbeta12Manalpha13Manalpha, has also been found in the mannan of Saccharomyces kluyveri but not in the mannan of Candida species. Furthermore, these oligosaccharides are branched at position 6 of the 3-O-substituted mannose units as follows.

and

The H-1 signals of the mannose units substituted by a 3,6-di-O-substituted unit showed a significant upfield shift (Delta = 0.04-0.08 ppm) due to a steric effect. The inhibition of an enzyme-linked immunosorbent assay between the mannan of C. guilliermondii and factor 9 serum with oligosaccharides obtained from several mannans indicated that only the oligosaccharides with the above structure were active, suggesting that these correspond to the epitope of antigenic factor 9.



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