(platelet membrane glycoprotein IIb-IIIa) and are members of the subfamily of integrin adhesion receptors. A cyclic peptide, KYGC(s-s)HarGDWPC(s-s) (cHarGD), originally described by Scarborough et al. (Scarborough, R. M., Naughton, M. A., Teng, W., Rose, J. W., Phillips, D. R., Nannizzi, L., Arsten, A., Campbell, A. M., and Charo, I. F.(1993) J. Biol. Chem. 268, 1066-1073) has been employed as a high affinity ligand for to examine the specificity of the integrins. cHarGD interacted with high affinity with purified (K = 10 nM) or with platelets (K = 120 nM). While cHarGD was specific for in the presence of Ca, it bound to both integrins in the presence of Mn. Barbourin, a snake venom disintegrin containing a reactive KGD sequence, remained -specific, even in the presence of Mn. cHarGD became cross-linked to a site in of , which is distinct from that of RGD peptides. These results allow identification of at least four classes of ligands: Class I, represented by RGD peptides and vitronectin, react similarly with and ; Class II, represented by cHarGD, -chain peptides and fibrinogen, react with both receptors in the presence of Mn but only with in the presence of Ca; Class III, represented by barbourin, are -specific under all cation conditions; Class IV, represented by osteopontin, bind primarily to .

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. M. Pesho, K. Bledzka, L. Michalec, C. S. Cierniewski, and E. F. Plow The Specificity and Function of the Metal-binding Sites in the Integrin beta3 A-domain J. Biol. Chem., August 11, 2006; 281(32): 23034 - 23041. [Abstract] [Full Text] [PDF]

				G. S. Daftary and H. S. Taylor Endocrine Regulation of HOX Genes Endocr. Rev., June 1, 2006; 27(4): 331 - 355. [Abstract] [Full Text] [PDF]

				T. Oki, J. Kitaura, K. Eto, Y. Lu, M. Maeda-Yamamoto, N. Inagaki, H. Nagai, Y. Yamanishi, H. Nakajina, H. Kumagai, et al. Integrin {alpha}IIb{beta}3 Induces the Adhesion and Activation of Mast Cells through Interaction with Fibrinogen J. Immunol., January 1, 2006; 176(1): 52 - 60. [Abstract] [Full Text] [PDF]

				J. Cho, J. L. Degen, B. S. Coller, and D. F. Mosher Fibrin but Not Adsorbed Fibrinogen Supports Fibronectin Assembly by Spread Platelets: EFFECTS OF THE INTERACTION OF {alpha}IIb{beta}3 WITH THE C TERMINUS OF THE FIBRINOGEN {gamma}-CHAIN J. Biol. Chem., October 21, 2005; 280(42): 35490 - 35498. [Abstract] [Full Text] [PDF]

				R. I. Litvinov, C. Nagaswami, G. Vilaire, H. Shuman, J. S. Bennett, and J. W. Weisel Functional and structural correlations of individual {alpha}IIb{beta}3 molecules Blood, December 15, 2004; 104(13): 3979 - 3985. [Abstract] [Full Text] [PDF]

				A. Artoni, J. Li, B. Mitchell, J. Ruan, J. Takagi, T. A. Springer, D. L. French, and B. S. Coller Inaugural Article: Integrin {beta}3 regions controlling binding of murine mAb 7E3: Implications for the mechanism of integrin {alpha}IIb{beta}3 activation PNAS, September 7, 2004; 101(36): 13114 - 13120. [Abstract] [Full Text] [PDF]

				P. H. Weinreb, K. J. Simon, P. Rayhorn, W. J. Yang, D. R. Leone, B. M. Dolinski, B. R. Pearse, Y. Yokota, H. Kawakatsu, A. Atakilit, et al. Function-blocking Integrin {alpha}v{beta}6 Monoclonal Antibodies: DISTINCT LIGAND-MIMETIC AND NONLIGAND-MIMETIC CLASSES J. Biol. Chem., April 23, 2004; 279(17): 17875 - 17887. [Abstract] [Full Text] [PDF]

				G.A. Stouffer and S.S. Smyth Effects of Thrombin on Interactions Between {beta}3-Integrins and Extracellular Matrix in Platelets and Vascular Cells Arterioscler. Thromb. Vasc. Biol., November 1, 2003; 23(11): 1971 - 1978. [Abstract] [Full Text] [PDF]

				J. Pilch, R. Habermann, and B. Felding-Habermann Unique Ability of Integrin alpha vbeta 3 to Support Tumor Cell Arrest under Dynamic Flow Conditions J. Biol. Chem., June 7, 2002; 277(24): 21930 - 21938. [Abstract] [Full Text] [PDF]

				Y.-F. Liao, P. J. Gotwals, V. E. Koteliansky, D. Sheppard, and L. Van De Water The EIIIA Segment of Fibronectin Is a Ligand for Integrins alpha 9beta 1 and alpha 4beta 1 Providing a Novel Mechanism for Regulating Cell Adhesion by Alternative Splicing J. Biol. Chem., April 19, 2002; 277(17): 14467 - 14474. [Abstract] [Full Text] [PDF]

				G. S. Daftary, P. J. Troy, C. N. Bagot, S. L. Young, and H. S. Taylor Direct Regulation of {beta}3-Integrin Subunit Gene Expression by HOXA10 in Endometrial Cells Mol. Endocrinol., March 1, 2002; 16(3): 571 - 579. [Abstract] [Full Text] [PDF]

				R. M. Scarborough, M. Lele, M. Sajid, N. Wajih, and G. A. Stouffer Eptifibatide and 7E3, but Not Tirofiban, Inhibit {alpha}v{beta}3 Integrin-Mediated Binding of Smooth Muscle Cells to Thrombospondin and Prothrombin Response Circulation, February 12, 2002; 105 (6): e46 - e46. [Full Text] [PDF]

				M. Lele, M. Sajid, N. Wajih, and G. A. Stouffer Eptifibatide and 7E3, but Not Tirofiban, Inhibit {alpha}v{beta}3 Integrin-Mediated Binding of Smooth Muscle Cells to Thrombospondin and Prothrombin Circulation, July 31, 2001; 104(5): 582 - 587. [Abstract] [Full Text] [PDF]

				T. M. Odrljin, C. G. Haidaris, N. B. Lerner, and P. J. Simpson-Haidaris Integrin {alpha}v{beta}3-Mediated Endocytosis of Immobilized Fibrinogen by A549 Lung Alveolar Epithelial Cells Am. J. Respir. Cell Mol. Biol., January 1, 2001; 24(1): 12 - 21. [Abstract] [Full Text]

				C. S. Cierniewski, T. Byzova, M. Papierak, T. A. Haas, J. Niewiarowska, L. Zhang, M. Cieslak, and E. F. Plow Peptide Ligands Can Bind to Distinct Sites in Integrin alpha IIbbeta 3 and Elicit Different Functional Responses J. Biol. Chem., June 11, 1999; 274(24): 16923 - 16932. [Abstract] [Full Text] [PDF]

				M. Quinn, A. Deering, M. Stewart, D. Cox, B. Foley, and D. Fitzgerald Quantifying GPIIb/IIIa Receptor Binding Using 2 Monoclonal Antibodies : Discriminating Abciximab and Small Molecular Weight Antagonists Circulation, May 4, 1999; 99(17): 2231 - 2238. [Abstract] [Full Text] [PDF]

				T. V. Byzova and E. F. Plow Activation of alpha Vbeta 3 on Vascular Cells Controls Recognition of Prothrombin J. Cell Biol., December 28, 1998; 143(7): 2081 - 2092. [Abstract] [Full Text] [PDF]

				J. S. Deitch, J. K. Williams, M. R. Adams, C. A. Fly, D. M. Herrington, R. E. Jordan, M. T. Nakada, J. A. Jakubowski, and R. L. Geary Effects of ί3-Integrin Blockade (c7E3) on the Response to Angioplasty and Intra-Arterial Stenting in Atherosclerotic Nonhuman Primates Arterioscler. Thromb. Vasc. Biol., November 1, 1998; 18(11): 1730 - 1737. [Abstract] [Full Text] [PDF]

				A. Wong, S. M. Hwang, K. Johanson, J. Samanen, D. Bennett, S. W. Landvatter, W. Chen, J. R. Heys, F. E. Ali, T. W. Ku, et al. Binding of [3H]-SK&F 107260 and [3H]-SB 214857 to Purified Integrin alpha IIbbeta 3: Evidence for a Common Binding Site for Cyclic Arginyl-Glycinyl-Aspartic Acid Peptides and Nonpeptides J. Pharmacol. Exp. Ther., April 1, 1998; 285(1): 228 - 235. [Abstract] [Full Text]

				X.-P. Zhang, T. Kamata, K. Yokoyama, W. Puzon-McLaughlin, and Y. Takada Specific Interaction of the Recombinant Disintegrin-like Domain of MDC-15 (Metargidin, ADAM-15) with Integrin alpha vbeta 3 J. Biol. Chem., March 27, 1998; 273(13): 7345 - 7350. [Abstract] [Full Text] [PDF]

				M. L. Kireeva, S. C.-T. Lam, and L. F. Lau Adhesion of Human Umbilical Vein Endothelial Cells to the Immediate-Early Gene Product Cyr61 Is Mediated through Integrin alpha vbeta 3 J. Biol. Chem., January 30, 1998; 273(5): 3090 - 3096. [Abstract] [Full Text] [PDF]

				B. Felding-Habermann, S. Silletti, F. Mei, C.-H. Siu, P. M. Yip, P. C. Brooks, D. A. Cheresh, T. E. O'Toole, M. H. Ginsberg, and A. M.P. Montgomery A Single Immunoglobulin-like Domain of the Human Neural Cell Adhesion Molecule L1 Supports Adhesion by Multiple Vascular and Platelet Integrins J. Cell Biol., December 15, 1997; 139(6): 1567 - 1581. [Abstract] [Full Text] [PDF]

				G. Chi-Rosso, P. J. Gotwals, J. Yang, L. Ling, K. Jiang, B. Chao, D. P. Baker, L. C. Burkly, S. E. Fawell, and V. E. Koteliansky Fibronectin Type III Repeats Mediate RGD-independent Adhesion and Signaling through Activated beta 1 Integrins J. Biol. Chem., December 12, 1997; 272(50): 31447 - 31452. [Abstract] [Full Text] [PDF]

				S.S. Srivatsa, L. A Fitzpatrick, P. W Tsao, T. M Reilly, D. R Holmes Jr, R. S Schwartz, and S. A Mousa Selective {alpha}v{beta}3 integrin blockade potently limits neointimal hyperplasia and lumen stenosis following deep coronary arterial stent injury:: Evidence for the functional importance of integrin {alpha}v{beta}3 and osteopontin expression during neointima formation Cardiovasc Res, December 1, 1997; 36(3): 408 - 428. [Abstract] [Full Text] [PDF]

				A. Byrne, N. Moran, M. Maher, N. Walsh, P. Crean, and D. J. Fitzgerald Continued Thromboxane A2 Formation Despite Administration of a Platelet Glycoprotein IIb/IIIa Antagonist in Patients Undergoing Coronary Angioplasty Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 3224 - 3229. [Abstract] [Full Text]

				K. Suehiro, J. Gailit, and E. F. Plow Fibrinogen Is a Ligand for Integrin alpha 5beta 1 on Endothelial Cells J. Biol. Chem., February 21, 1997; 272(8): 5360 - 5366. [Abstract] [Full Text] [PDF]

				T. J. Kunicki, D. S. Annis, and B. Felding-Habermann Molecular Determinants of Arg-Gly-Asp Ligand Specificity for beta 3 Integrins J. Biol. Chem., February 14, 1997; 272(7): 4103 - 4107. [Abstract] [Full Text] [PDF]