|
|
|
|||||||
|
|||||||||
(Received for publication, October 16, 1995; and in revised form, January 16, 1996) Acid phosphatases (Acp) of intracellular pathogens have recently
been implicated as virulence factors that enhance intracellular
survival through suppression of the respiratory burst. We describe here
the identification, purification, characterization, and sequencing of a
novel burst-inhibiting acid phosphatase from the facultative
intracellular bacterium, Francisella tularensis. Similar to
other the burst-inhibiting Acps, F. tularensis Acp (AcpA) is
tartrate-resistant and has broad substrate specificity. The AcpA enzyme
is unique, however, in that it is easily released from the bacterial
cell in soluble form, is a basic enzyme, suppresses the respiratory
burst of not only fMet-Leu-Phe but also phorbol 12-myristate
13-acetate-stimulated neutrophils and does not fit into any of the
three currently recognized classes of acid phosphatase. We also report
the complete nucleotide sequence of the gene acpA, encoding
AcpA, and the deduced primary structure of its encoded polypeptide.
Comparative sequence analyses of AcpA is discussed. To our knowledge,
this is the first report describing the cloning and sequencing of a
burst-inhibiting acid phosphatase.
Volume 271,
Number 18,
Issue of May 3, 1996 pp. 10973-10983
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. W. Buchan, R. L. McCaffrey, S. R. Lindemann, L.-A. H. Allen, and B. D. Jones Identification of migR, a Regulatory Element of the Francisella tularensis Live Vaccine Strain iglABCD Virulence Operon Required for Normal Replication and Trafficking in Macrophages Infect. Immun., June 1, 2009; 77(6): 2517 - 2529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Reilly, D. L. Chance, M. J. Calcutt, J. J. Tanner, R. L. Felts, S. C. Waller, M. T. Henzl, T. P. Mawhinney, I. K. Ganjam, and W. H. Fales Characterization of a Unique Class C Acid Phosphatase from Clostridium perfringens Appl. Envir. Microbiol., June 1, 2009; 75(11): 3745 - 3754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Schmerk, B. N. Duplantis, P. L. Howard, and F. E. Nano A Francisella novicida pdpA mutant exhibits limited intracellular replication and remains associated with the lysosomal marker LAMP-1 Microbiology, May 1, 2009; 155(5): 1498 - 1504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Schulert, R. L. McCaffrey, B. W. Buchan, S. R. Lindemann, C. Hollenback, B. D. Jones, and L.-A. H. Allen Francisella tularensis Genes Required for Inhibition of the Neutrophil Respiratory Burst and Intramacrophage Growth Identified by Random Transposon Mutagenesis of Strain LVS Infect. Immun., April 1, 2009; 77(4): 1324 - 1336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chong, T. D. Wehrly, V. Nair, E. R. Fischer, J. R. Barker, K. E. Klose, and J. Celli The Early Phagosomal Stage of Francisella tularensis Determines Optimal Phagosomal Escape and Francisella Pathogenicity Island Protein Expression Infect. Immun., December 1, 2008; 76(12): 5488 - 5499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. I. Richards, S. L. Michell, and P. C. F. Oyston An intracellularly inducible gene involved in virulence and polyphosphate production in Francisella J. Med. Microbiol., October 1, 2008; 57(10): 1183 - 1192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. P. Mohapatra, S. Soni, T. J. Reilly, J. Liu, K. E. Klose, and J. S. Gunn Combined Deletion of Four Francisella novicida Acid Phosphatases Attenuates Virulence and Macrophage Vacuolar Escape Infect. Immun., August 1, 2008; 76(8): 3690 - 3699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Brotcke and D. M. Monack Identification of fevR, a Novel Regulator of Virulence Gene Expression in Francisella novicida Infect. Immun., August 1, 2008; 76(8): 3473 - 3480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. F. Oyston Francisella tularensis: unravelling the secrets of an intracellular pathogen J. Med. Microbiol., August 1, 2008; 57(8): 921 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Horzempa, D. M. Tarwacki, P. E. Carlson Jr., C. M. Robinson, and G. J. Nau Characterization and Application of a Glucose-Repressible Promoter in Francisella tularensis Appl. Envir. Microbiol., April 1, 2008; 74(7): 2161 - 2170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Hoopman, W. Wang, C. A. Brautigam, J. L. Sedillo, T. J. Reilly, and E. J. Hansen Moraxella catarrhalis Synthesizes an Autotransporter That Is an Acid Phosphatase J. Bacteriol., February 15, 2008; 190(4): 1459 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ventura, C. Canchaya, A. Tauch, G. Chandra, G. F. Fitzgerald, K. F. Chater, and D. van Sinderen Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum Microbiol. Mol. Biol. Rev., September 1, 2007; 71(3): 495 - 548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Lindemann, M. K. McLendon, M. A. Apicella, and B. D. Jones An In Vitro Model System Used To Study Adherence and Invasion of Francisella tularensis Live Vaccine Strain in Nonphagocytic Cells Infect. Immun., June 1, 2007; 75(6): 3178 - 3182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. P. Mohapatra, A. Balagopal, S. Soni, L. S. Schlesinger, and J. S. Gunn AcpA Is a Francisella Acid Phosphatase That Affects Intramacrophage Survival and Virulence Infect. Immun., January 1, 2007; 75(1): 390 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Felts, T. J. Reilly, and J. J. Tanner Structure of Francisella tularensis AcpA: PROTOTYPE OF A UNIQUE SUPERFAMILY OF ACID PHOSPHATASES AND PHOSPHOLIPASES C J. Biol. Chem., October 6, 2006; 281(40): 30289 - 30298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Maier, R. Pechous, M. Casey, T. C. Zahrt, and D. W. Frank In Vivo Himar1-Based Transposon Mutagenesis of Francisella tularensis. Appl. Envir. Microbiol., March 1, 2006; 72(3): 1878 - 1885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Vanittanakom, C. R. Cooper Jr., M. C. Fisher, and T. Sirisanthana Penicillium marneffei Infection and Recent Advances in the Epidemiology and Molecular Biology Aspects Clin. Microbiol. Rev., January 1, 2006; 19(1): 95 - 110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tauch, O. Kaiser, T. Hain, A. Goesmann, B. Weisshaar, A. Albersmeier, T. Bekel, N. Bischoff, I. Brune, T. Chakraborty, et al. Complete Genome Sequence and Analysis of the Multiresistant Nosocomial Pathogen Corynebacterium jeikeium K411, a Lipid-Requiring Bacterium of the Human Skin Flora J. Bacteriol., July 1, 2005; 187(13): 4671 - 4682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Maier, A. Havig, M. Casey, F. E. Nano, D. W. Frank, and T. C. Zahrt Construction and Characterization of a Highly Efficient Francisella Shuttle Plasmid Appl. Envir. Microbiol., December 1, 2004; 70(12): 7511 - 7519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Abd, T. Johansson, I. Golovliov, G. Sandstrom, and M. Forsman Survival and Growth of Francisella tularensis in Acanthamoeba castellanii Appl. Envir. Microbiol., January 1, 2003; 69(1): 600 - 606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ellis, P. C. F. Oyston, M. Green, and R. W. Titball Tularemia Clin. Microbiol. Rev., October 1, 2002; 15(4): 631 - 646. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Taramelli, C. Tognazioli, F. Ravagnani, O. Leopardi, G. Giannulis, and J. R. Boelaert Inhibition of Intramacrophage Growth of Penicillium marneffei by 4-Aminoquinolines Antimicrob. Agents Chemother., May 1, 2001; 45(5): 1450 - 1455. [Abstract] [Full Text]
|
|
|
|
|
|
V. Aragon, S. Kurtz, and N. P. Cianciotto Legionella pneumophila Major Acid Phosphatase and Its Role in Intracellular Infection Infect. Immun., January 1, 2001; 69(1): 177 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Saleh and J. T. Belisle Secretion of an Acid Phosphatase (SapM) by Mycobacterium tuberculosis That Is Similar to Eukaryotic Acid Phosphatases J. Bacteriol., December 1, 2000; 182(23): 6850 - 6853. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. U. Ahmed, E. Rojo, V. Kovaleva, S. Venkataraman, J. E. Dombrowski, K. Matsuoka, and N. V. Raikhel The Plant Vacuolar Sorting Receptor AtELP Is Involved in Transport of NH2-terminal Propeptide-containing Vacuolar Proteins in Arabidopsis thaliana J. Cell Biol., June 26, 2000; 149(7): 1335 - 1344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Aragon, S. Kurtz, A. Flieger, B. Neumeister, and N. P. Cianciotto Secreted Enzymatic Activities of Wild-Type and pilD-Deficient Legionella pneumophila Infect. Immun., April 1, 2000; 68(4): 1855 - 1863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Reilly, D. L. Chance, and A. L. Smith Outer Membrane Lipoprotein e (P4) of Haemophilus influenzae Is a Novel Phosphomonoesterase J. Bacteriol., November 1, 1999; 181(21): 6797 - 6805. [Abstract] [Full Text]
|
|
|
|
|
|
L. S. Terada, K. A. Johansen, S. Nowbar, A. I. Vasil, and M. L. Vasil Pseudomonas aeruginosa Hemolytic Phospholipase C Suppresses Neutrophil Respiratory Burst Activity Infect. Immun., May 1, 1999; 67(5): 2371 - 2376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Malke Cytoplasmic Membrane Lipoprotein LppC of Streptococcus equisimilis Functions as an Acid Phosphatase Appl. Envir. Microbiol., July 1, 1998; 64(7): 2439 - 2442. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |