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(Received for publication, December 29,
1995; and in revised form, February 22, 1996) Alternative splicing of vertebrate
Volume 271,
Number 19,
Issue of May 10, 1996 pp. 11511-11517
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
-Tropomyosin Alternative Exon
-tropomyosin transcripts
ensures mutually exclusive expression of internal exons 6A and 6B in
nonmuscle and skeletal muscle cells, respectively. Recently, we
reported that this splicing regulation requires species-specific
elements, since the splicing profile for the chicken, rat, and Xenopus
-tropomyosin alternative exons is not reproduced
in transfection experiments when heterologous myogenic cells are used.
By analyzing the splicing pattern of hybrid chicken/rat
-TM
constructions transfected into both quail and mouse cell lines, we
demonstrate that chicken
-tropomyosin exon 6A is flanked by
stronger splicing signals than rat exon 6A, thus leading to the
misregulation of splicing in heterologous cells. We have characterized
three splicing signals that contribute to this difference: 1)
nonconsensus nucleotide differences at positions +4 and +6 in
the donor site downstream of exon 6A, 2) differences in the pyrimidine
composition between the branch site and acceptor site upstream of exon
6A, and 3) a pyrimidine-rich intronic exon 6A splicing enhancer present
upstream of exon 6A only in the chicken
-TM gene. The functional
divergence between splicing signals in two homologous vertebrate genes
reveals species-specific strategies for proper modulation of splicing
of alternative exons.
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