A cloned rat epithelial Na channel (rENaC) was studied in planar lipid bilayers. Two forms of the channel were examined: channels produced by the subunit alone and those formed by , , and subunits. The protein was derived from two sources: either from in vitro translation reaction followed by Sephadex column purification or from heterologous expression in Xenopus oocytes and isolation of plasma membranes. We found that either -rENaC alone or - in combination with - and -rENaC, produced highly Na-selective (P/P = 10), amiloride-sensitive (K = 170 nM), and mechanosensitive cation channels in planar bilayers. -rENaC displayed a complicated gating mechanism: there was a nearly constitutively open 13-picosiemens (pS) state and a second 40-pS level that was achieved from the 13-pS level by a 26-pS transition. -, -, -rENaC showed primarily the 13-pS level. -rENaC and ,,-rENaC channels studied by patch clamp displayed the same gating pattern, albeit with >2-fold lowered conductance levels, i.e. 6 and 18 pS, respectively. Upon treatment of either channel with the sulfhydryl reducing agent dithiothreitol, both channels fluctuated among three independent 13-pS sublevels. Bathing each channel with a high salt solution (1.5 M NaCl) produced stochastic openings of 19 and 38 pS in magnitude between all three conductance levels. Different combinations of -, -, and -rENaC in the reconstitution mixture did not produce channels of intermediate conductance levels. These findings suggest that functional ENaC is composed of three identical conducting elements and that their gating is concerted.

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