HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by MacDonald, R. J. Articles by Kroon, E. Search for Related Content PubMed PubMed Citation Articles by MacDonald, R. J. Articles by Kroon, E. Social Bookmarking                      What's this?

Volume 271, Number 23, Issue of June 7, 1996 pp. 13684-13690
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Disparate Tissue-specific Expression of Members of the Tissue Kallikrein Multigene Family of the Rat

(Received for publication, December 29, 1995, and in revised form, February 29, 1996)

From the Department of Biochemistry, Molecular Immunology Center, the University of Texas Southwestern Medical Center, Dallas, Texas 75235-9140

To understand the regulatory diversity of the rat family of linked kallikrein genes, we have assayed the expression of family members in 20 major organs. Reverse transcription-polymerase chain reaction analysis using primers and hybridization probes specific for each of the 10 expressed kallikrein genes showed that no two family members share the same organ-specific pattern of expression. The only common site of expression for all 10 known active genes is the submandibular gland. The presence of the mRNA for at least one family member is detected in 19 of these 20 organs (liver excepted), from as few as three organs to as many as 18 for individual family members. For individual genes there can be more than a 10⁵-fold variation in mRNA levels among organs, from a limit of detection of slightly less than 1 mRNA molecule/10 cells to more than 10,000 mRNA molecules/cell. Despite high sequence conservation and close linkage, the members of this family are expressed in very different and complex patterns. A gradient of diversity of expression corresponds to the order of the genes within the kallikrein family locus.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				X. S. Puente and C. Lopez-Otin A Genomic Analysis of Rat Proteases and Protease Inhibitors Genome Res., April 1, 2004; 14(4): 609 - 622. [Abstract] [Full Text] [PDF]

				T. K. Monsees, S. Blocher, F. Heidorn, A. Winkler, W.-E. Siems, W. Muller-Esterl, J. Hayatpour, W. Miska, and W.-B. Schill Expression and Location of the Bradykinin B2 Receptor in Rat Testis Biol Reprod, December 1, 2002; 67(6): 1832 - 1839. [Abstract] [Full Text] [PDF]

				Y. Liu, M. P. Patricelli, and B. F. Cravatt Activity-based protein profiling: The serine hydrolases PNAS, December 21, 1999; 96(26): 14694 - 14699. [Abstract] [Full Text] [PDF]

				M. G. Lee, P. J Schultheis, M. Yan, G. E Shull, C. Bookstein, E. Chang, M. Tse, M. Donowitz, K. Park, and S. Muallem Membrane-limited expression and regulation of Na+-H+ exchanger isoforms by P2 receptors in the rat submandibular gland duct J. Physiol., December 1, 1998; 513(2): 341 - 357. [Abstract] [Full Text] [PDF]

				T. J. Harvey, J. D. Hooper, S. A. Myers, S.-A. Stephenson, L. K. Ashworth, and J. A. Clements Tissue-specific Expression Patterns and Fine Mapping of the Human Kallikrein (KLK) Locus on Proximal 19q13.4 J. Biol. Chem., November 22, 2000; 275(48): 37397 - 37406. [Abstract] [Full Text] [PDF]