HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ikuta, T. Articles by Kan, Y. W. Search for Related Content PubMed PubMed Citation Articles by Ikuta, T. Articles by Kan, Y. W. Social Bookmarking                      What's this?

Volume 271, Number 24, Issue of June 14, 1996 pp. 14082-14091
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Globin Gene Switching
IN VIVO PROTEIN-DNA INTERACTIONS OF THE HUMAN -GLOBIN LOCUS IN ERYTHROID CELLS EXPRESSING THE FETAL OR THE ADULT GLOBIN GENE PROGRAM

(Received for publication, January 31, 1996, and in revised form, March 19, 1996)

Tohru Ikuta , Thalia Papayannopoulou ^¶ , George Stamatoyannopoulos ^¶ and Yuet Wai Kan

From the  Department of Laboratory Medicine and  Howard Hughes Medical Institute, University of California, San Francisco, California 94143 and the ^¶ Division of Medical Genetics, University of Washington, Seattle, Washington 98195

To characterize the protein-DNA interactions important for the developmental control of the human -globin locus, we analyzed by in vivo dimethyl sulfate footprinting erythroid cells expressing either the fetal or the adult globin developmental program. In the locus control region (LCR) of the -globin locus, in vivo footprints on NF-E2 (or AP-1) and GATA-1 motifs remained the same regardless of whether the fetal or the adult globin genes are expressed. In contrast, in vivo footprints on GT (CACCC) motifs differed between the cells expressing the fetal or the adult globin program. In promoter regions, the actively transcribed genes demonstrated extensive and consistent footprints over the canonical elements, such as CACCC and CCAAT motifs. The adult globin expressing cells displayed more extensive footprints than the fetal globin expressing cells in the 3 regulatory sequences of both the ^A- and the -globin genes, suggesting a role of these 3 elements in -globin gene expression. Our results suggest that the bulk of protein-DNA interactions that underlies the developmental control of globin genes takes place in the - and -globin gene promoters, and that GT motifs of the -globin locus LCR may play a role in the developmental regulation of human -globin gene expression, perhaps by increasing the probability of interaction of the LCR holocomplex with the fetal or the adult globin gene.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. Feng and Y. W. Kan The binding of the ubiquitous transcription factor Sp1 at the locus control region represses the expression of {beta}-like globin genes PNAS, July 12, 2005; 102(28): 9896 - 9900. [Abstract] [Full Text] [PDF]

				P. A. Navas, R. A. Swank, M. Yu, K. R. Peterson, and G. Stamatoyannopoulos Mutation of a transcriptional motif of a distant regulatory element reduces the expression of embryonic and fetal globin genes Hum. Mol. Genet., November 15, 2003; 12(22): 2941 - 2948. [Abstract] [Full Text] [PDF]

				C. E. Horak, M. C. Mahajan, N. M. Luscombe, M. Gerstein, S. M. Weissman, and M. Snyder GATA-1 binding sites mapped in the beta -globin locus by using mammalian chIp-chip analysis PNAS, February 20, 2002; (2002) 52706999. [Abstract] [Full Text] [PDF]

				M. C. Mahajan and S. M. Weissman DNA-dependent adenosine triphosphatase (helicaselike transcription factor) activates beta -globin transcription in K562 cells Blood, January 1, 2002; 99(1): 348 - 356. [Abstract] [Full Text] [PDF]

				R. H. Broyles, V. Belegu, C. R. DeWitt, S. N. Shah, C. A. Stewart, Q. N. Pye, and R. A. Floyd Specific repression of beta -globin promoter activity by nuclear ferritin PNAS, July 31, 2001; 98(16): 9145 - 9150. [Abstract] [Full Text] [PDF]

				T. Ikuta, S. Ausenda, and M. D. Cappellini Mechanism for fetal globin gene expression: Role of the soluble guanylate cyclase-cGMP-dependent protein kinase pathway PNAS, February 1, 2001; (2001) 41599798. [Abstract] [Full Text]

				H. Asano, X. S. Li, and G. Stamatoyannopoulos FKLF, a Novel Kruppel-Like Factor That Activates Human Embryonic and Fetal beta -Like Globin Genes Mol. Cell. Biol., May 1, 1999; 19(5): 3571 - 3579. [Abstract] [Full Text] [PDF]

				T. Ikuta, Y. W. Kan, P. S. Swerdlow, D. V. Faller, and S. P. Perrine Alterations in Protein-DNA Interactions in the gamma -Globin Gene Promoter in Response to Butyrate Therapy Blood, October 15, 1998; 92(8): 2924 - 2933. [Abstract] [Full Text] [PDF]

				P. Pasceri, D. Pannell, X. Wu, and J. Ellis Full Activity From Human beta -Globin Locus Control Region Transgenes Requires 5'HS1, Distal beta -Globin Promoter, and 3' beta -Globin Sequences Blood, July 15, 1998; 92(2): 653 - 663. [Abstract] [Full Text] [PDF]

				H. A. a. G. Stamatoyannopoulos Activation of beta -Globin Promoter by Erythroid Kruppel-Like Factor Mol. Cell. Biol., January 1, 1998; 18(1): 102 - 109. [Abstract] [Full Text]

				S. Iwamoto, H. Suganuma, T. Kamesaki, T. Omi, H. Okuda, and E. Kajii Cloning and Characterization of Erythroid-specific DNase I-hypersensitive Site in Human Rhesus-associated Glycoprotein Gene J. Biol. Chem., August 25, 2000; 275(35): 27324 - 27331. [Abstract] [Full Text] [PDF]

				T. Ikuta, S. Ausenda, and M. D. Cappellini Mechanism for fetal globin gene expression: Role of the soluble guanylate cyclase-cGMP-dependent protein kinase pathway PNAS, February 13, 2001; 98(4): 1847 - 1852. [Abstract] [Full Text] [PDF]

				C. E. Horak, M. C. Mahajan, N. M. Luscombe, M. Gerstein, S. M. Weissman, and M. Snyder GATA-1 binding sites mapped in the beta -globin locus by using mammalian chIp-chip analysis PNAS, March 5, 2002; 99(5): 2924 - 2929. [Abstract] [Full Text] [PDF]