| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, April 8, 1996, and in revised form, April 24, 1996)
From the We have investigated the cell death of a
Chinese hamster ovary mutant (MT-58) with a thermo-sensitive
CTP:phosphocholine cytidylyltransferase, the rate-limiting enzyme of
the CDP-choline pathway for phosphatidylcholine biosynthesis (Esko, J. D., Wermuth, M. M., and Raetz, C. R. H. (1981) J. Biol.
Chem. 256, 7388-7393). After MT-58 cells were shifted to the
restrictive temperature of 40° C, the cytidylyltransferase was
inactivated immediately leading to a decrease in phosphatidylcholine
biosynthesis and cell death. DNA content and number of cells in the S
phase decreased significantly in the dying MT-58 cells according to
flow cytometrical analyses. The fragmentation of genomic DNA was
detected by DNA ladders in agarose gel and release of the prelabeled
genomic DNA into cytosolic fractions 14 h after the temperature
shift. The dying cells underwent a dramatic reduction of cellular
volume while maintaining the membrane containment of cellular contents.
These events indicated that the inactivation of cytidylyltransferase
triggered apoptosis in Chinese hamster ovary cells. This is the first
report that apoptosis was induced in cultured cells, not by an added
agent, but by a mutation in phospholipid biosynthesis.
Volume 271, Number 25,
Issue of June 21, 1996
pp. 14668-14671
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
COMMUNICATION:
,
,
,
,
and
Lipid and Lipoprotein Research
Group and Department of Biochemistry, ¶ Surgical-Medical Research
Institute, University of Alberta, Edmonton, Alberta T6G 2S2, Canada and
the 
INSERM Unité 326, Hôpital Purpan, 31059
Toulouse cedex, France
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. J. Ryan, B. B. Chen, P. R. Vennalaganti, F. C. Henderson, L. A. Tephly, A. B. Carter, and R. K. Mallampalli 15-Deoxy-{Delta}12,14-prostaglandin J2 Impairs Phosphatidylcholine Synthesis and Induces Nuclear Accumulation of Thiol-modified Cytidylyltransferase J. Biol. Chem., September 5, 2008; 283(36): 24628 - 24640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Vance, Z. Li, and R. L. Jacobs Hepatic Phosphatidylethanolamine N-Methyltransferase, Unexpected Roles in Animal Biochemistry and Physiology J. Biol. Chem., November 16, 2007; 282(46): 33237 - 33241. [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tian, R. Zhou, J. E. Rehg, and S. Jackowski Role of Phosphocholine Cytidylyltransferase {alpha} in Lung Development Mol. Cell. Biol., February 1, 2007; 27(3): 975 - 982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. McCoy, K. Fisher, J. Robichaud, A. J. Ryan, and R. K. Mallampalli Transcriptional Regulation of Lung Cytidylyltransferase in Developing Transgenic Mice Am. J. Respir. Cell Mol. Biol., September 1, 2006; 35(3): 394 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Banchio, S. Lingrell, and D. E. Vance Role of Histone Deacetylase in the Expression of CTP:Phosphocholine Cytidylyltransferase {alpha} J. Biol. Chem., April 14, 2006; 281(15): 10010 - 10015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bao, A. Bohrer, S. Ramanadham, W. Jin, S. Zhang, and J. Turk Effects of Stable Suppression of Group VIA Phospholipase A2 Expression on Phospholipid Content and Composition, Insulin Secretion, and Proliferation of INS-1 Insulinoma Cells J. Biol. Chem., January 6, 2006; 281(1): 187 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-G. Choi, V. Kurnov, M. C. Kersting, A. Sreenivas, and G. M. Carman Phosphorylation of the Yeast Choline Kinase by Protein Kinase C: IDENTIFICATION OF Ser25 AND Ser30 AS MAJOR SITES OF PHOSPHORYLATION J. Biol. Chem., July 15, 2005; 280(28): 26105 - 26112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Scaglia and R. A. Igal Stearoyl-CoA Desaturase Is Involved in the Control of Proliferation, Anchorage-independent Growth, and Survival in Human Transformed Cells J. Biol. Chem., July 8, 2005; 280(27): 25339 - 25349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. Shen, C. J. DeLong, F. Terce, T. Kute, M. C. Willingham, M. J. Pettenati, and Z. Cui Polyploid Formation via Chromosome Duplication Induced by CTP:Phosphocholine Cytidylyltransferase Deficiency and Bcl-2 Overexpression: Identification of Two Novel Endogenous Factors J. Histochem. Cytochem., June 1, 2005; 53(6): 725 - 733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, S. Magdaleno, I. Tabas, and S. Jackowski Early Embryonic Lethality in Mice with Targeted Deletion of the CTP:Phosphocholine Cytidylyltransferase {alpha} Gene (Pcyt1a) Mol. Cell. Biol., April 15, 2005; 25(8): 3357 - 3363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Vance and D. E. Vance Metabolic Insights into Phospholipid Function Using Gene-targeted Mice J. Biol. Chem., March 25, 2005; 280(12): 10877 - 10880. [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Lagace and N. D. Ridgway The Rate-limiting Enzyme in Phosphatidylcholine Synthesis Regulates Proliferation of the Nucleoplasmic Reticulum Mol. Biol. Cell, March 1, 2005; 16(3): 1120 - 1130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jackowski and P. Fagone CTP:Phosphocholine Cytidylyltransferase: Paving the Way from Gene to Membrane J. Biol. Chem., January 14, 2005; 280(2): 853 - 856. [Full Text] [PDF]
|
|
|
|
|
|
M. H. M. van der Sanden, H. Meems, M. Houweling, J. B. Helms, and A. B. Vaandrager Induction of CCAAT/Enhancer-binding Protein (C/EBP)-homologous Protein/Growth Arrest and DNA Damage-inducible Protein 153 Expression during Inhibition of Phosphatidylcholine Synthesis Is Mediated via Activation of a C/EBP-activating Transcription Factor-responsive Element J. Biol. Chem., December 10, 2004; 279(50): 52007 - 52015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wu, B. Lau, S. Smith, K. Troyan, and D. E. Barnett Foster Enteropathogenic Escherichia coli Infection Triggers Host Phospholipid Metabolism Perturbations Infect. Immun., December 1, 2004; 72(12): 6764 - 6772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Banchio, L. M. Schang, and D. E. Vance Phosphorylation of Sp1 by Cyclin-dependent Kinase 2 Modulates the Role of Sp1 in CTP:Phosphocholine Cytidylyltransferase {alpha} Regulation during the S Phase of the Cell Cycle J. Biol. Chem., September 17, 2004; 279(38): 40220 - 40226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Caviglia, I. N. T. de Gomez Dumm, R. A. Coleman, and R. A. Igal Phosphatidylcholine deficiency upregulates enzymes of triacylglycerol metabolism in CHO cells J. Lipid Res., August 1, 2004; 45(8): 1500 - 1509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cruz-Ramirez, J. Lopez-Bucio, G. Ramirez-Pimentel, A. Zurita-Silva, L. Sanchez-Calderon, E. Ramirez-Chavez, E. Gonzalez-Ortega, and L. Herrera-Estrella The xipotl Mutant of Arabidopsis Reveals a Critical Role for Phospholipid Metabolism in Root System Development and Epidermal Cell Integrity PLANT CELL, August 1, 2004; 16(8): 2020 - 2034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zweigner, S. Jackowski, S. H. Smith, M. van der Merwe, J. R. Weber, and E. I. Tuomanen Bacterial Inhibition of Phosphatidylcholine Synthesis Triggers Apoptosis in the Brain J. Exp. Med., July 6, 2004; 200(1): 99 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-S. Choi, A. Sreenivas, G.-S. Han, and G. M. Carman Regulation of Phospholipid Synthesis in the Yeast cki1{Delta} eki1{Delta} Mutant Defective in the Kennedy Pathway: THE CHO1-ENCODED PHOSPHATIDYLSERINE SYNTHASE IS REGULATED BY mRNA STABILITY J. Biol. Chem., March 26, 2004; 279(13): 12081 - 12087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhou, Y. You, J. Zabner, A. J. Ryan, and R. K. Mallampalli The CCT Promoter Directs High-Level Transgene Expression in Distal Lung Epithelial Cell Lines Am. J. Respir. Cell Mol. Biol., January 1, 2004; 30(1): 61 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Fuentes, R. Perez, M. L. Nieto, J. Balsinde, and M. A. Balboa Bromoenol Lactone Promotes Cell Death by a Mechanism Involving Phosphatidate Phosphohydrolase-1 Rather than Calcium-independent Phospholipase A2 J. Biol. Chem., November 7, 2003; 278(45): 44683 - 44690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ekroos, C. S. Ejsing, U. Bahr, M. Karas, K. Simons, and A. Shevchenko Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation J. Lipid Res., November 1, 2003; 44(11): 2181 - 2192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Banchio, L. M. Schang, and D. E. Vance Activation of CTP:Phosphocholine Cytidylyltransferase {alpha} Expression during the S Phase of the Cell Cycle Is Mediated by the Transcription Factor Sp1 J. Biol. Chem., August 22, 2003; 278(34): 32457 - 32464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Lee, Y.-M. Han, B.-H. Min, and I.-S. Park Cytoprotective Effects of Polyenoylphosphatidylcholine (PPC) on {beta}-cells During Diabetic Induction by Streptozotocin J. Histochem. Cytochem., August 1, 2003; 51(8): 1005 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ramos, M. E. Mouedden, E. Claro, and S. Jackowski Inhibition of CTP:Phosphocholine Cytidylyltransferase by C2-Ceramide and Its Relationship to Apoptosis Mol. Pharmacol., November 1, 2002; 62(5): 1068 - 1075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. van der Luit, M. Budde, P. Ruurs, M. Verheij, and W. J. van Blitterswijk Alkyl-lysophospholipid Accumulates in Lipid Rafts and Induces Apoptosis via Raft-dependent Endocytosis and Inhibition of Phosphatidylcholine Synthesis J. Biol. Chem., October 11, 2002; 277(42): 39541 - 39547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Lagace, J. R. Miller, and N. D. Ridgway Caspase Processing and Nuclear Export of CTP:Phosphocholine Cytidylyltransferase {alpha} during Farnesol-Induced Apoptosis Mol. Cell. Biol., July 1, 2002; 22(13): 4851 - 4862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gennero, J. Fauvel, M. Nieto, C. Cariven, F. Gaits, F. Briand-Mesange, H. Chap, and J. P. Salles Apoptotic Effect of Sphingosine 1-Phosphate and Increased Sphingosine 1-Phosphate Hydrolysis on Mesangial Cells Cultured at Low Cell Density J. Biol. Chem., April 5, 2002; 277(15): 12724 - 12734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Vieu, F. Terce, F. Chevy, C. Rolland, R. Barbaras, H. Chap, C. Wolf, B. Perret, and X. Collet Coupled assay of sphingomyelin and ceramide molecular species by gas liquid chromatography J. Lipid Res., March 1, 2002; 43(3): 510 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Morvan, A. Demidem, J. Papon, M. De Latour, and J. C. Madelmont Melanoma Tumors Acquire a New Phospholipid Metabolism Phenotype under Cystemustine As Revealed by High-Resolution Magic Angle Spinning Proton Nuclear Magnetic Resonance Spectroscopy of Intact Tumor Samples Cancer Res., March 1, 2002; 62(6): 1890 - 1897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Waite, N. R. Cabilio, and D. E. Vance Choline Deficiency-Induced Liver Damage Is Reversible in Pemt-/- Mice J. Nutr., January 1, 2002; 132(1): 68 - 71. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Golfman, M. Bakovic, and D. E. Vance Transcription of the CTP:Phosphocholine Cytidylyltransferase alpha Gene Is Enhanced during the S Phase of the Cell Cycle J. Biol. Chem., November 16, 2001; 276(47): 43688 - 43692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Adibhatla, J.F. Hatcher, and R.J. Dempsey Effects of Citicoline on Phospholipid and Glutathione Levels in Transient Cerebral Ischemia Stroke, October 1, 2001; 32(10): 2376 - 2381. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-L. E. YEN, M.-H. MAR, R. B. MEEKER, A. FERNANDES, and S. H. ZEISEL Choline deficiency induces apoptosis in primary cultures of fetal neurons FASEB J, August 1, 2001; 15(10): 1704 - 1710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. VRABLIC, C. D. ALBRIGHT, C. N. CRACIUNESCU, R. I. SALGANIK, and S. H. ZEISEL Altered mitochondrial function and overgeneration of reactive oxygen species precede the induction of apoptosis by 1-O-octadecyl-2-methyl-rac-glycero-3-phosphocholine in p53-defective hepatocytes FASEB J, August 1, 2001; 15(10): 1739 - 1744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Finney, E. Nudelman, T. White, S. Bursten, P. Klein, L. L. Leer, N. Wang, D. Waggoner, J. W. Singer, and R. A. Lewis Pharmacological Inhibition of Phosphatidylcholine Biosynthesis Is Associated with Induction of Phosphatidylinositol Accumulation and Cytolysis of Neoplastic Cell Lines Cancer Res., September 1, 2000; 60(18): 5204 - 5213. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. S. Attard, R. H. Templer, W. S. Smith, A. N. Hunt, and S. Jackowski Modulation of CTP:phosphocholine cytidylyltransferase by membrane curvature elastic stress PNAS, July 19, 2000; (2000) 160260697. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Emoto and M. Umeda An Essential Role for a Membrane Lipid in Cytokinesis: Regulation of Contractile Ring Disassembly by Redistribution of Phosphatidylethanolamine J. Cell Biol., June 12, 2000; 149(6): 1215 - 1224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. C. Northwood, A. H. Y. Tong, B. Crawford, A. E. Drobnies, and R. B. Cornell Shuttling of CTP:Phosphocholine Cytidylyltransferase between the Nucleus and Endoplasmic Reticulum Accompanies the Wave of Phosphatidylcholine Synthesis during the G0 right-arrow G1 Transition J. Biol. Chem., September 10, 1999; 274(37): 26240 - 26248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Anthony, M. Zhao, and K. M. Brindle Inhibition of Phosphatidylcholine Biosynthesis following Induction of Apoptosis in HL-60 Cells J. Biol. Chem., July 9, 1999; 274(28): 19686 - 19692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Tessitore, E. Sesca, M. Bosco, and D. E. Vance Expression of phosphatidylethanolamine N-methyltransferase in Yoshida ascites hepatoma cells and the livers of host rats Carcinogenesis, April 1, 1999; 20(4): 561 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miquel, A. Pradines, F. Terce, S. Selmi, and G. Favre Competitive Inhibition of Choline Phosphotransferase by Geranylgeraniol and Farnesol Inhibits Phosphatidylcholine Synthesis and Induces Apoptosis in Human Lung Adenocarcinoma A549 Cells J. Biol. Chem., October 2, 1998; 273(40): 26179 - 26186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wieder, C. E. Orfanos, and C. C. Geilen Induction of Ceramide-mediated Apoptosis by the Anticancer Phospholipid Analog, Hexadecylphosphocholine J. Biol. Chem., May 1, 1998; 273(18): 11025 - 11031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Baburina and S. Jackowski Apoptosis Triggered by 1-O-Octadecyl-2-O-methyl-rac-glycero-3-phosphocholine Is Prevented by Increased Expression of CTP:Phosphocholine Cytidylyltransferase J. Biol. Chem., January 23, 1998; 273(4): 2169 - 2173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-i. Atsumi, M. Murakami, K. Kojima, A. Hadano, M. Tajima, and I. Kudo Distinct Roles of Two Intracellular Phospholipase A2s in Fatty Acid Release in the Cell Death Pathway. PROTEOLYTIC FRAGMENT OF TYPE IVA CYTOSOLIC PHOSPHOLIPASE A2alpha INHIBITS STIMULUS-INDUCED ARACHIDONATE RELEASE, WHEREAS THAT OF TYPE VI Ca2+-INDEPENDENT PHOSPHOLIPASE A2 AUGMENTS SPONTANEOUS FATTY ACID RELEASE J. Biol. Chem., June 9, 2000; 275(24): 18248 - 18258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Waite and D. E. Vance Why Expression of Phosphatidylethanolamine N-Methyltransferase Does Not Rescue Chinese Hamster Ovary Cells That Have an Impaired CDP-Choline Pathway J. Biol. Chem., July 7, 2000; 275(28): 21197 - 21202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Dowd, M. E. Bier, and J. L. Patton-Vogt Turnover of Phosphatidylcholine in Saccharomyces cerevisiae. THE ROLE OF THE CDP-CHOLINE PATHWAY J. Biol. Chem., February 2, 2001; 276(6): 3756 - 3763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. DeLong, L. Qin, and Z. Cui Nuclear Localization of Enzymatically Active Green Fluorescent Protein-CTP:Phosphocholine Cytidylyltransferase alpha Fusion Protein Is Independent of Cell Cycle Conditions and Cell Types J. Biol. Chem., October 6, 2000; 275(41): 32325 - 32330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Wright, A. L. Henneberry, T. A. Lagace, N. D. Ridgway, and C. R. McMaster Uncoupling Farnesol-induced Apoptosis from Its Inhibition of Phosphatidylcholine Synthesis J. Biol. Chem., June 29, 2001; 276(27): 25254 - 25261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Attard, R. H. Templer, W. S. Smith, A. N. Hunt, and S. Jackowski Modulation of CTP:phosphocholine cytidylyltransferase by membrane curvature elastic stress PNAS, August 1, 2000; 97(16): 9032 - 9036. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
