HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, J. Articles by Pratt, R. E. Search for Related Content PubMed PubMed Citation Articles by Zhang, J. Articles by Pratt, R. E. Social Bookmarking                      What's this?

Volume 271, Number 25, Issue of June 21, 1996 pp. 15026-15033
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

The AT₂ Receptor Selectively Associates with G_i2 and G_i3 in the Rat Fetus

(Received for publication, March 7, 1996)

From the Division of Cardiovascular Medicine, Falk Cardiovascular Research Center, Stanford University School of Medicine, Stanford, California 94305-5246

The effects of angiotensin II are mediated by a family of seven transmembrane receptors. In the adult, the majority of the receptors are of the AT₁ isoform, which is coupled to heterotrimeric G proteins (either G_q or G_i). In contrast, the AT₂ receptor is expressed at low levels in the adult but is the major form expressed in the fetal and neonatal animal. Previous results have failed to show G protein coupling of the AT₂ receptor in the fetus. We now provide evidence that the AT₂ receptor is G protein-coupled. An antibody that binds several G subunits immunoselected angiotensin II receptor-G complexes. In addition, G_i1-3 antibody, which recognizes G_i1, G_i2 and G_i3, also co-immunoselect the AT₂ receptor. Anti-G_i2 and anti-G_i3 antibodies were both able to co-immunoselected AT₂ receptor-G_i complexes, but consistent with the lack of G_i1 in the fetal extracts, anti-G_i1 antibodies did not nor did any other G protein-directed antisera. The finding that AT₂ receptor couples to both G_i2 and G_i3 raises the possibility that selective interactions between AT₂ receptor and different G proteins may result in specific cellular effects mediated by AT₂ stimulation.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				X. Zhang, G. Wang, D. J. Dupre, Y. Feng, M. Robitaille, E. Lazartigues, Y.-H. Feng, T. E. Hebert, and G. Wu Rab1 GTPase and Dimerization in the Cell Surface Expression of Angiotensin II Type 2 Receptor J. Pharmacol. Exp. Ther., July 1, 2009; 330(1): 109 - 117. [Abstract] [Full Text] [PDF]

				W Zhao, D I Diz, and M E Robbins Oxidative damage pathways in relation to normal tissue injury Br. J. Radiol., September 1, 2007; 80(Special_Issue_1): S23 - S31. [Abstract] [Full Text] [PDF]

				X. Li, K. M. Lerea, J. Li, and S. C. Olson Src Kinase Mediates Angiotensin II-Dependent Increase in Pulmonary Endothelial Nitric Oxide Synthase Am. J. Respir. Cell Mol. Biol., September 1, 2004; 31(3): 365 - 372. [Abstract] [Full Text] [PDF]

				D.-F. Guo, V. Tardif, K. Ghelima, J. S. D. Chan, J. R. Ingelfinger, X. Chen, and I. Chenier A Novel Angiotensin II Type 1 Receptor-associated Protein Induces Cellular Hypertrophy in Rat Vascular Smooth Muscle and Renal Proximal Tubular Cells J. Biol. Chem., May 14, 2004; 279(20): 21109 - 21120. [Abstract] [Full Text] [PDF]

				B. T. Andresen, G. G. Romero, and E. K. Jackson AT2 Receptors Attenuate AT1 Receptor-Induced Phospholipase D Activation in Vascular Smooth Muscle Cells J. Pharmacol. Exp. Ther., April 1, 2004; 309(1): 425 - 431. [Abstract] [Full Text]

				R. Benndorf, R. H. Boger, S. Ergun, A. Steenpass, and T. Wieland Angiotensin II Type 2 Receptor Inhibits Vascular Endothelial Growth Factor-Induced Migration and In Vitro Tube Formation of Human Endothelial Cells Circ. Res., September 5, 2003; 93(5): 438 - 447. [Abstract] [Full Text] [PDF]

				L. Gendron, J.-F. Oligny, M. D. Payet, and N. Gallo-Payet Cyclic AMP-independent Involvement of Rap1/B-Raf in the Angiotensin II AT2 Receptor Signaling Pathway in NG108-15 Cells J. Biol. Chem., January 31, 2003; 278(6): 3606 - 3614. [Abstract] [Full Text] [PDF]

				Y.-H. Feng, Y. Sun, and J. G. Douglas Gbeta gamma -independent constitutive association of Galpha s with SHP-1 and angiotensin II receptor AT2 is essential in AT2-mediated ITIM-independent activation of SHP-1 PNAS, September 17, 2002; 99(19): 12049 - 12054. [Abstract] [Full Text] [PDF]

				C. Berry, R. Touyz, A. F. Dominiczak, R. C. Webb, and D. G. Johns Angiotensin receptors: signaling, vascular pathophysiology, and interactions with ceramide Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2337 - H2365. [Abstract] [Full Text] [PDF]

				Chiming Wei, M. G Cardarelli, S. W Downing, and J. S McLaughlin The effect of angiotensin II on mitogen-activated protein kinase in human cardiomyocytes Journal of Renin-Angiotensin-Aldosterone System, December 1, 2000; 1(4): 379 - 384. [Abstract] [PDF]

				L. Breault, E. Chamoux, J.-G. LeHoux, and N. Gallo-Payet Localization of G Protein {{alpha}}-Subunits in the Human Fetal Adrenal Gland Endocrinology, December 1, 2000; 141(12): 4334 - 4341. [Abstract] [Full Text] [PDF]

				J. A. Olivares-Reyes, S. Jayadev, L. Hunyady, K. J. Catt, and R. D. Smith Homologous and Heterologous Phosphorylation of the AT2 Angiotensin Receptor by Protein Kinase C Mol. Pharmacol., November 1, 2000; 58(5): 1156 - 1161. [Abstract] [Full Text]

				J. L. Hansen, G. Servant, T. J. Baranski, T. Fujita, T. Iiri, and S. P. Sheikh Functional Reconstitution of the Angiotensin II Type 2 Receptor and Gi Activation Circ. Res., October 27, 2000; 87(9): 753 - 759. [Abstract] [Full Text] [PDF]

				M. de Gasparo, K. J. Catt, T. Inagami, J. W. Wright, and Th. Unger International Union of Pharmacology. XXIII. The Angiotensin II Receptors Pharmacol. Rev., September 1, 2000; 52(3): 415 - 472. [Abstract] [Full Text] [PDF]

				S. Kim and H. Iwao Molecular and Cellular Mechanisms of Angiotensin II-Mediated Cardiovascular and Renal Diseases Pharmacol. Rev., March 1, 2000; 52(1): 11 - 34. [Abstract] [Full Text] [PDF]

				S. Gallinat, S. Busche, M. K. Raizada, and C. Sumners The angiotensin II type 2 receptor: an enigma with multiple variations Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E357 - E374. [Abstract] [Full Text] [PDF]

				R. M. Carey, Z.-Q. Wang, and H. M. Siragy Role of the Angiotensin Type 2 Receptor in the Regulation of Blood Pressure and Renal Function Hypertension, January 1, 2000; 35(1): 155 - 163. [Abstract] [Full Text] [PDF]

				R. M. Rajagopalan-Gupta, S. Mukherjee, X. Zhu, Y.-K. Ho, H. Hamm, M. Birnbaumer, L. Birnbaumer, and M. Hunzicker-Dunn Roles of Gi and Gq/11 in Mediating Desensitization of the Luteinizing Hormone/Choriogonadotropin Receptor in Porcine Ovarian Follicular Membranes Endocrinology, April 1, 1999; 140(4): 1612 - 1621. [Abstract] [Full Text]

				M. Horiuchi, M. Akishita, and V. J. Dzau Recent Progress in Angiotensin II Type 2 Receptor Research in the Cardiovascular System Hypertension, February 1, 1999; 33(2): 613 - 621. [Abstract] [Full Text] [PDF]

				H. Matsubara Pathophysiological Role of Angiotensin II Type 2 Receptor in Cardiovascular and Renal Diseases Circ. Res., December 14, 1998; 83(12): 1182 - 1191. [Abstract] [Full Text] [PDF]

				Y. Tsutsumi, H. Matsubara, N. Ohkubo, Y. Mori, Y. Nozawa, S. Murasawa, K. Kijima, K. Maruyama, H. Masaki, Y. Moriguchi, et al. Angiotensin II Type 2 Receptor Is Upregulated in Human Heart With Interstitial Fibrosis, and Cardiac Fibroblasts Are the Major Cell Type for Its Expression Circ. Res., November 16, 1998; 83(10): 1035 - 1046. [Abstract] [Full Text] [PDF]

				M. Zhu, C. H. Gelband, J. M. Moore, P. Posner, and C. Sumners Angiotensin II Type 2 Receptor Stimulation of Neuronal Delayed-Rectifier Potassium Current Involves Phospholipase A2 and Arachidonic Acid J. Neurosci., January 15, 1998; 18(2): 679 - 686. [Abstract] [Full Text] [PDF]

				M. van Bilsen Signal transduction revisited: recent developments in angiotensin II signaling in the cardiovascular system Cardiovasc Res, December 1, 1997; 36(3): 310 - 322. [Full Text] [PDF]

				S. Conchon, M.-B. Barrault, S. Miserey, P. Corvol, and E. Clauser The C-terminal Third Intracellular Loop of the Rat AT1A Angiotensin Receptor Plays a Key Role in G Protein Coupling Specificity and Transduction of the Mitogenic Signal J. Biol. Chem., October 10, 1997; 272(41): 25566 - 25572. [Abstract] [Full Text] [PDF]

				H. Sun, Z. Chen, H. Poppleton, K. Scholich, J. Mullenix, G. J. Weipz, D. L. Fulgham, P. J. Bertics, and T. B. Patel The Juxtamembrane, Cytosolic Region of the Epidermal Growth Factor Receptor Is Involved in Association with alpha -Subunit of Gs J. Biol. Chem., February 28, 1997; 272(9): 5413 - 5420. [Abstract] [Full Text] [PDF]

				A. Abel, N. Wittau, T. Wieland, G. Schultz, and F. Kalkbrenner Cell Cycle-dependent Coupling of the Vasopressin V1a Receptor to Different G Proteins J. Biol. Chem., October 13, 2000; 275(42): 32543 - 32551. [Abstract] [Full Text] [PDF]