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(Received for publication, January 19, 1996, and in revised form, April 17, 1996)
From the Institute of Anatomy, University of Freiburg, D-79104
Freiburg, Federal Republic of Germany
In order to clarify the physiological function of
fibroblast growth factor (FGF-2) in the adrenal medulla the regulation
of FGF-2 and FGF receptor 1 (FGFR1) was studied in vitro
and in vivo in response to glucocorticoids. To assess the
effects of glucocorticoids, in vivo extracts of adrenal
medulla and adrenal cortex were analyzed by RNase protection assay and
Western blot analysis. PC12 cells were chosen as a model system to
study the effects of glucocorticoids in vitro. In PC12
cells, dexamethasone (DEX) was found to stimulate dramatically the
expression of both FGF-2 mRNA and protein. Western blot analysis
revealed that exclusively the 21-kDa FGF-2 isoform was enhanced. In
contrast to the FGF-2 mRNA level FGFR1 was not affected by
treatment with glucocorticoids. In vivo FGF-2 mRNA
level and 21-kDa FGF-2 isoform level are significantly enhanced in the
adrenal medulla 24 h after DEX injection. In vivo
application of DEX leads to an increase of the medullary and cortical
FGFR1 transcript levels. Glucocorticoid effects on FGF-2 expression
were not found in adrenal cortex, heart, skeletal muscle, and kidney,
respectively, in vivo and in L6 rat myoblasts in
vitro.
In addition to adrenal medullary cells glucocorticoids elevated the
FGF-2 mRNA and protein level also in vivo in the brain
and in vitro in immortalized Schwann cells. The present
results suggest that the 21-kDa FGF-2 isoform mediates a physiological
function specific for neuronal tissue which is modulated by
glucocorticoids.
Volume 271, Number 28,
Issue of July 12, 1996
pp. 16520-16525
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
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