| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, February 29, 1996, and in revised form, April 29, 1996)
From the The Src family protein-tyrosine kinases are
required for mitogenic signaling from the platelet-derived growth
factor (PDGF), colony stimulating factor-1, and epidermal growth factor
(EGF) receptor protein-tyrosine kinases (RPTK) (Twamley-Stein, G. M.,
Pepperkok, R., Ansorge, W., and Courtneidge, S. A. (1993) Proc.
Natl. Acad. Sci. U. S. A. 90, 7696-7700; Roche, S., Koegl, M.,
Barone, M. V., Roussel, M. F., and Courtneidge, S. A. (1995) Mol.
Cell. Biol. 15, 1102-1109). In NIH3T3 fibroblasts, c-Src, Fyn,
and c-Yes associate with the activated PDGF receptor, are substrates
for receptor phosphorylation, and are themselves activated. Src family
catalytic function is required for RPTK mitogenic signaling as
evidenced by the SH2-dependent dominant negative phenotype
exhibited by kinase-inactive Src and Fyn mutants (Twamley-Stein, G. M.,
Pepperkok, R., Ansorge, W., and Courtneidge, S. A. (1993) Proc.
Natl. Acad. Sci. U. S. A. 90, 7696-7700). Here, we have
generated clonal Src
Volume 271, Number 28,
Issue of July 12, 1996
pp. 16798-16806
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
§
and
Molecular Biology and Virology Laboratory,
The Salk Institute, La Jolla, California 92037 and the
§ Department of Biology, University of California, San
Diego, La Jolla, California 92093
murine fibroblast cell lines
overexpressing various murine c-Src mutants and studied the effect of
these mutant Src proteins on PDGF- and EGF-induced mitogenesis. Two
c-Src SH3 domain mutants, Y133F and Y138F, each inhibited PDGF BB- and
EGF-induced DNA synthesis in quiescent cells. This demonstrates an
involvement of the Src SH3 domain in PDGF
and EGF receptor mitogenic
signaling. Since both Tyr-133 and Tyr-138 are located on the ligand
binding surface of the SH3 domain, these results suggest that the c-Src
SH3 domain is required for PDGF and EGF mitogenic signaling. The
dominant negative effect of either single mutant on PDGF receptor
signaling was reversed by a second SH2-inactivating mutation. We
conclude that the c-Src SH3 domain function requires the SH2 domain in
the case of the PDGF receptor, presumably because binding of c-Src to
the receptor via its SH2 domain is a prerequisite for the SH3 domain
function. In contrast, SH2 function is apparently not essential for the
SH3 function in EGF receptor signaling.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Vidal, S. Warner, R. Read, and R. L. Cagan Differing Src Signaling Levels Have Distinct Outcomes in Drosophila Cancer Res., November 1, 2007; 67(21): 10278 - 10285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Shvartsman, J. C. Donaldson, B. Diaz, O. Gutman, G. S. Martin, and Y. I. Henis Src kinase activity and SH2 domain regulate the dynamics of Src association with lipid and protein targets J. Cell Biol., August 9, 2007; 178(4): 675 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Rahmani APRO4 negatively regulates Src tyrosine kinase activity in PC12 cells J. Cell Sci., February 15, 2006; 119(4): 646 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gentili, R. Boland, and A. R. de Boland Implication of G{beta}{gamma} proteins and c-SRC tyrosine kinase in parathyroid hormone-induced signal transduction in rat enterocytes J. Endocrinol., January 1, 2006; 188(1): 69 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Niu, K. L. Wright, Y. Ma, G. M. Wright, M. Huang, R. Irby, J. Briggs, J. Karras, W. D. Cress, D. Pardoll, et al. Role of Stat3 in Regulating p53 Expression and Function Mol. Cell. Biol., September 1, 2005; 25(17): 7432 - 7440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Munugalavadla, L. C. Dore, B. L. Tan, L. Hong, M. Vishnu, M. J. Weiss, and R. Kapur Repression of c-Kit and Its Downstream Substrates by GATA-1 Inhibits Cell Proliferation during Erythroid Maturation Mol. Cell. Biol., August 1, 2005; 25(15): 6747 - 6759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Li, C. Marshall, L. Mei, L. Dzubow, C. Schmults, M. Dans, and J. Seykora Srcasm Modulates EGF and Src-kinase Signaling in Keratinocytes J. Biol. Chem., February 18, 2005; 280(7): 6036 - 6046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Nixon Prion-associated Increases in Src-family Kinases J. Biol. Chem., January 28, 2005; 280(4): 2455 - 2462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. O'Neill, J. Gillett, and K. R. Foltz Distinct roles for multiple Src family kinases at fertilization J. Cell Sci., December 1, 2004; 117(25): 6227 - 6238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ren, S. Meng, L. Mei, Z. J. Zhao, R. Jove, and J. Wu Roles of Gab1 and SHP2 in Paxillin Tyrosine Dephosphorylation and Src Activation in Response to Epidermal Growth Factor J. Biol. Chem., February 27, 2004; 279(9): 8497 - 8505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Feranchak, G. Kilic, P. A. Wojtaszek, I. Qadri, and J. G. Fitz Volume-sensitive Tyrosine Kinases Regulate Liver Cell Volume through Effects on Vesicular Trafficking and Membrane Na+ Permeability J. Biol. Chem., November 7, 2003; 278(45): 44632 - 44638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Takikita-Suzuki, M. Haneda, M. Sasahara, M. K. Owada, T. Nakagawa, M. Isono, S. Takikita, D. Koya, K. Ogasawara, and R. Kikkawa Activation of Src Kinase in Platelet-Derived Growth Factor-B-Dependent Tubular Regeneration after Acute Ischemic Renal Injury Am. J. Pathol., July 1, 2003; 163(1): 277 - 286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kong, C. Mounier, V. Dumas, and B. I. Posner Epidermal Growth Factor-induced DNA Synthesis. KEY ROLE FOR Src PHOSPHORYLATION OF THE DOCKING PROTEIN Gab2 J. Biol. Chem., February 14, 2003; 278(8): 5837 - 5844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Schmitt and P. J. S. Stork Galpha and Gbeta gamma Require Distinct Src-dependent Pathways to Activate Rap1 and Ras J. Biol. Chem., November 1, 2002; 277(45): 43024 - 43032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Cheriyath, Z. P. Desgranges, and A. L. Roy c-Src-dependent Transcriptional Activation of TFII-I J. Biol. Chem., June 14, 2002; 277(25): 22798 - 22805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Kesavan, C. C. Isaacson, C. L. Ashendel, R. L. Geahlen, and M. L. Harrison Characterization of the in Vivo Sites of Serine Phosphorylation on Lck Identifying Serine 59 as a Site of Mitotic Phosphorylation J. Biol. Chem., April 19, 2002; 277(17): 14666 - 14673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Selbach, S. Moese, C. R. Hauck, T. F. Meyer, and S. Backert Src Is the Kinase of the Helicobacter pylori CagA Protein in Vitro and in Vivo J. Biol. Chem., February 22, 2002; 277(9): 6775 - 6778. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P. Eliceiri Integrin and Growth Factor Receptor Crosstalk Circ. Res., December 7, 2001; 89(12): 1104 - 1110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Boney, H. Sekimoto, P. A. Gruppuso, and A. R. Frackelton Jr. Src Family Tyrosine Kinases Participate in Insulin-like Growth Factor I Mitogenic Signaling in 3T3-L1 Cells Cell Growth Differ., July 1, 2001; 12(7): 379 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Bowman, M. A. Broome, D. Sinibaldi, W. Wharton, W. J. Pledger, J. M. Sedivy, R. Irby, T. Yeatman, S. A. Courtneidge, and R. Jove Stat3-mediated Myc expression is required for Src transformation and PDGF-induced mitogenesis PNAS, June 7, 2001; (2001) 131568898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bouchard, S. Giannakopoulos, E. H. Wang, N. Tanese, and R. J. Schneider Hepatitis B Virus HBx Protein Activation of Cyclin A-Cyclin-Dependent Kinase 2 Complexes and G1 Transit via a Src Kinase Pathway J. Virol., May 1, 2001; 75(9): 4247 - 4257. [Abstract] [Full Text]
|
|
|
|
|
|
A. W.-M. Lee and D. J. States Both Src-Dependent and -Independent Mechanisms Mediate Phosphatidylinositol 3-Kinase Regulation of Colony-Stimulating Factor 1-Activated Mitogen-Activated Protein Kinases in Myeloid Progenitors Mol. Cell. Biol., September 15, 2000; 20(18): 6779 - 6798. [Abstract] [Full Text]
|
|
|
|
|
|
C. Bondzi, J. Litz, P. Dent, and G. W. Krystal Src Family Kinase Activity Is Required for Kit-mediated Mitogen-activated Protein (MAP) Kinase Activation, However Loss of Functional Retinoblastoma Protein Makes MAP Kinase Activation Unnecessary for Growth of Small Cell Lung Cancer Cells Cell Growth Differ., June 1, 2000; 11(6): 305 - 314. [Abstract] [Full Text]
|
|
|
|
|
|
G. Manes, P. Bello, and S. Roche Slap Negatively Regulates Src Mitogenic Function but Does Not Revert Src-Induced Cell Morphology Changes Mol. Cell. Biol., May 15, 2000; 20(10): 3396 - 3406. [Abstract] [Full Text]
|
|
|
|
|
|
C.-C. Lai, C. Henningson, and D. DiMaio Bovine Papillomavirus E5 Protein Induces the Formation of Signal Transduction Complexes Containing Dimeric Activated Platelet-derived Growth Factor beta Receptor and Associated Signaling Proteins J. Biol. Chem., March 24, 2000; 275(13): 9832 - 9840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Furge, K. Chen, and S. Cohen Annexin VII and Annexin XI Are Tyrosine Phosphorylated in Peroxovanadate-treated Dogs and in Platelet-derived Growth Factor-treated Rat Vascular Smooth Muscle Cells J. Biol. Chem., November 19, 1999; 274(47): 33504 - 33509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Plattner, L. Kadlec, K. A. DeMali, A. Kazlauskas, and A. M. Pendergast c-Abl is activated by growth factors and Src family kinases and has a role in the cellular response to PDGF Genes & Dev., September 15, 1999; 13(18): 2400 - 2411. [Abstract] [Full Text]
|
|
|
|
|
|
L. A. Sellers Prolonged Activation of Extracellular Signal-regulated Kinase by a Protein Kinase C-dependent and N17Ras-insensitive Mechanism Mediates the Proliferative Response of Gi/o-coupled Somatostatin sst4 Receptors J. Biol. Chem., August 20, 1999; 274(34): 24280 - 24288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-K. Qu, W.-M. Yu, B. Azzarelli, and G.-S. Feng Genetic evidence that Shp-2 tyrosine phosphatase is a signal enhancer of the epidermal growth factor receptor in mammals PNAS, July 20, 1999; 96(15): 8528 - 8533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-W. Chow, M. Woodside, N. Demaurex, F. H. Yu, P. Plant, D. Rotin, S. Grinstein, and J. Orlowski Proline-rich Motifs of the Na+/H+ Exchanger 2 Isoform. BINDING OF Src HOMOLOGY DOMAIN 3 AND ROLE IN APICAL TARGETING IN EPITHELIA J. Biol. Chem., April 9, 1999; 274(15): 10481 - 10488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Blake, P. Garcia-Paramio, P. J. Parker, and S. A. Courtneidge Src Promotes PKC{{delta}} Degradation Cell Growth Differ., April 1, 1999; 10(4): 231 - 241. [Abstract] [Full Text]
|
|
|
|
|
|
N. Rahimi, W. Hung, E. Tremblay, R. Saulnier, and B. Elliott c-Src Kinase Activity Is Required for Hepatocyte Growth Factor-induced Motility and Anchorage-independent Growth of Mammary Carcinoma Cells J. Biol. Chem., December 11, 1998; 273(50): 33714 - 33721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Walker, A. Kato, L. J. Gonez, M. L. Hibbs, N. Pouliot, A. Levitzki, and A. W. Burgess Activation of the Ras/Mitogen-Activated Protein Kinase Pathway by Kinase-Defective Epidermal Growth Factor Receptors Results in Cell Survival but Not Proliferation Mol. Cell. Biol., December 1, 1998; 18(12): 7192 - 7204. [Abstract] [Full Text]
|
|
|
|
|
|
A. Herrlich, H. Daub, A. Knebel, P. Herrlich, A. Ullrich, G. Schultz, and T. Gudermann Ligand-independent activation of platelet-derived growth factor receptor is a necessary intermediate in lysophosphatidic, acid-stimulated mitogenic activity in L cells PNAS, July 21, 1998; 95(15): 8985 - 8990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. DeMali and A. Kazlauskas Activation of Src Family Members Is Not Required for the Platelet-Derived Growth Factor beta Receptor To Initiate Mitogenesis Mol. Cell. Biol., April 1, 1998; 18(4): 2014 - 2022. [Abstract] [Full Text]
|
|
|
|
|
|
F. R. Postma, T. Hengeveld, J. Alblas, B. N.G. Giepmans, G. C.M. Zondag, K. Jalink, and W. H. Moolenaar Acute loss of Cell-Cell Communication Caused by G Protein-coupled Receptors: A Critical Role for c-Src J. Cell Biol., March 9, 1998; 140(5): 1199 - 1209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Gelderloos, S. Rosenkranz, C. Bazenet, and A. Kazlauskas A Role for Src in Signal Relay by the Platelet-derived Growth Factor alpha Receptor J. Biol. Chem., March 6, 1998; 273(10): 5908 - 5915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Q. Hu, N. Singh, D. Mukhopadhyay, and H. I. Akbarali Modulation of Voltage-dependent Ca2+ Channels in Rabbit Colonic Smooth Muscle Cells by c-Src and Focal Adhesion Kinase J. Biol. Chem., February 27, 1998; 273(9): 5337 - 5342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jalali, Y.-S. Li, M. Sotoudeh, S. Yuan, S. Li, S. Chien, and J. Y-J. Shyy Shear Stress Activates p60src-Ras-MAPK Signaling Pathways in Vascular Endothelial Cells Arterioscler. Thromb. Vasc. Biol., February 1, 1998; 18(2): 227 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Schwartzberg, L. Xing, O. Hoffmann, C. A. Lowell, L. Garrett, B. F. Boyce, and H. E. Varmus Rescue of osteoclast function by transgenic expression of kinase-deficient Src in src-/- mutant mice Genes & Dev., November 1, 1997; 11(21): 2835 - 2844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Erpel, G. Alonso, S. Roche, and S. A. Courtneidge The Src SH3 Domain Is Required for DNA Synthesis Induced by Platelet-derived Growth Factor and Epidermal Growth Factor J. Biol. Chem., July 12, 1996; 271(28): 16807 - 16812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Y. W. Bourguignon, H. Zhu, L. Shao, and Y.-W. Chen CD44 Interaction with c-Src Kinase Promotes Cortactin-mediated Cytoskeleton Function and Hyaluronic Acid-dependent Ovarian Tumor Cell Migration J. Biol. Chem., March 2, 2001; 276(10): 7327 - 7336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Chung, J. Y. Sung, W. Ahn, H. Rhim, T. H. Oh, M. G. Lee, and Y. S. Ahn Intracellular Calcium Mobilization Induces Immediate Early Gene pip92 via Src and Mitogen-activated Protein Kinase in Immortalized Hippocampal Cells J. Biol. Chem., January 12, 2001; 276(3): 2132 - 2138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Hauck, T. Hunter, and D. D. Schlaepfer The v-Src SH3 Domain Facilitates a Cell Adhesion-independent Association with Focal Adhesion Kinase J. Biol. Chem., May 18, 2001; 276(21): 17653 - 17662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Bowman, M. A. Broome, D. Sinibaldi, W. Wharton, W. J. Pledger, J. M. Sedivy, R. Irby, T. Yeatman, S. A. Courtneidge, and R. Jove Stat3-mediated Myc expression is required for Src transformation and PDGF-induced mitogenesis PNAS, June 19, 2001; 98(13): 7319 - 7324. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
